草地学报

草地学报 ›› 2012, Vol. 20 ›› Issue (3): 524-529.DOI: 10.11733/j.issn.1007-0435.2012.03.022

• 研究论文 • 上一篇    下一篇

野生黄花苜蓿组织培养及再生体系研究

侯永霞, 王俊杰, 赵彦, 云锦凤, 陈雪英   

  1. 内蒙古农业大学生态环境学院 内蒙古自治区草品种育繁工程技术研究中心 草地资源教育部重点实验室, 内蒙古 呼和浩特 010019
  • 收稿日期:2011-11-11 修回日期:2012-02-27 出版日期:2012-06-15 发布日期:2012-07-05
  • 通讯作者: 王俊杰,E-mail:jjw62@sina.com
  • 作者简介:侯永霞(1987- ),女,内蒙古乌兰察布人,硕士研究生,主要从事牧草遗传育种研究,E-mail:tuzilove0511370133@163.com
  • 基金资助:
    国家"863"课题(2009AA10Z108 );国家自然科学基金项目(31060323);内蒙古自然科学基金项目(2009MS0404)资助

Tissue Culture and Regeneration System of Native Medicago falcata L.

HOU Yong-xia, WANG Jun-jie, ZHAO Yan, YUN Jin-feng, CHEN Xue-ying   

  1. College of Ecology and Environmental Science, Inner Mongolia Agricultural University; Inner Mongolia Engineering Technology Research Center for Forage Breeding and Reproduction;The Ministry of Education Key Laboratory of Grassland Resources, Huhhot, Inner Mongolia 010019, China
  • Received:2011-11-11 Revised:2012-02-27 Online:2012-06-15 Published:2012-07-05

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摘要： 以呼伦贝尔草原野生黄花苜蓿(Medicago falcata L.)无菌苗的子叶和下胚轴为外植体,对其组织培养及再生体系进行系统的研究,以期为其下一步转基因试验提供良好的受体。结果表明:最佳愈伤诱导培养基为MS+1.5 mg·L-1 2,4-D+0.4 mg·L-1 6-BA+3%蔗糖+0.7%琼脂,下胚轴和子叶的最佳愈伤诱导率均可达到100%。最佳胚性愈伤形成培养基为MS+0.5 mg·L-1 KT+0.1 mg·L-1 NAA+2%蔗糖+0.7%琼脂,胚性愈伤形成率为81.5%;最佳分化芽形成培养基为MS+0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2%蔗糖+0.7%琼脂,分化芽形成率为36.5%。最佳生根培养基为1/2MS+0.5 mg·L-1 NAA+1.5%蔗糖+0.7%琼脂,生根率为93.3%。愈伤诱导结果显示,供试黄花苜蓿材料个体间组织培养再生性存在较大差异,在同一激素水平上有大约1/3植株的愈伤状态优于其他植株。

关键词: 黄花苜蓿, 组织培养, 再生体系, 愈伤

Abstract: Using cotyledons and hypocotyls of wild Medicago falcata L. from Hulunbeier grassland as explants, tissue culture and regeneration system of Medicago falcata L. were studied for further transgenic experiments providing a good receptor. Results showed that the optimal medium for callus induction was MS+1.5 mg·L-1 2,4-D+0.4 mg·L-1 6-BA+3% sucrose+0.7% agar, the highest callus induction ratio was 100%. The optimal medium for embryogenic callus was MS+0.5 mg·L-1 KT+0.1 mg·L-1 NAA+2% sucrose+0.7% agar, inducted ratio of embryogenic callus was 81.5%.The optimal medium for differentiated buds was MS+0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2% sucrose+0.7% agar, differentiated ratio of buds was 36.5%. The optimal rooting medium was 1/2MS+0.5 mg·L-1 NAA+1.5% sucrose+0.7% agar, rooting ratio was 93.3%. Callus induction results showed that the tissue culture of Medicago falcata L. had different induction ratio between individuals with the same hormone.

Key words: Medicago falcata L., Tissue culture, Regeneration system, Callus

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