草地学报

草地学报 ›› 2016, Vol. 24 ›› Issue (3): 577-580.DOI: 10.11733/j.issn.1007-0435.2016.03.014

• 研究论文 • 上一篇    下一篇

干旱胁迫对苎麻苗期理化指标的影响

孙鏖1,2, 邓荟芬3, 刘海林2, 易康乐2, 李剑波2, 伍佰鑫2, 李昊帮2, 蒋桂韬1,2, 戴求仲1,2   

  1. 1. 中国农业科学院麻类研究所, 湖南 长沙 410205;
    2. 湖南畜牧兽医研究所, 湖南 长沙 410131;
    3. 湖南省生物机电职业技术学院, 湖南 长沙 410130
  • 收稿日期:2015-04-24 修回日期:2015-12-29 出版日期:2016-06-15 发布日期:2016-09-18
  • 通讯作者: 戴求仲
  • 作者简介:孙鏖(1982-),男,湖南邵阳人,硕士,助理研究员,主要从事牧草高产栽培与利用研究工作,E-mail:604612838@qq.com
  • 基金资助:

    中国农业科学院科技创新工程（ASTIP-IBFC02）资助

The Effects of Drought Stress on Physicochemical Index of the Seedling-Stage Ramie

SUN Ao1,2, DENG Hui-fen3, LIU Hai-lin2, YI Kang-le2, LI Jian-bo2, WU Bai-xin2, LI Hao-bang2, JIANG Gui-tao1,2, DAI Qiu-zhong1,2   

  1. 1. Institute of Bast Fiber Crops Chinese Academy of Agricultural Sciences, Changsha, Hunan Province 410205, China;
    2. Hunan Institute of Animal and Veterinary Science, Changsha, Hunan Province 410131, China;
    3. Hunan Biological and Electromechanical Polytechnic, Changsha, Hunan Province 410130, China
  • Received:2015-04-24 Revised:2015-12-29 Online:2016-06-15 Published:2016-09-18

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摘要：

用PEG-6000对苎麻（Boehmeria nivea ）幼苗进行不同时间的干旱胁迫，测定胁迫后第0 d，2 d，4 d，6 d及8 d内其叶片生理生化指标，包括游离脯氨酸（Pro）、过氧化物酶（POD）、丙二醛（MDA）和过氧化氢酶（CAT）的活性，细胞质膜透性、叶绿素含量及内源激素的变化。结果表明：随时间延长，Pro，POD和CAT活性呈先升高后降低的趋势；细胞膜透性和MDA活性增加；叶绿素含量降低；赤霉素（GA）和吲哚乙酸（IAA）含量下降；脱落酸（ABA）含量上升。因此，苎麻的保护系统能够进行自身的调节以抵抗干旱伤害。

关键词: 干旱胁迫, 苎麻, 酶, 内源激素, 叶绿素, 理化指标

Abstract:

The leaf physiological and biochemical indexes, including the activity changes of the free proline (Pro), peroxidase (POD), malondialdehyde (MDA) and activity of catalase (CAT), were measured within 0 to 8 d after treated with PEG-6000 as a drought stress on the seedling stage of ramie (Boehmeria nivea). The changes of the cytoplasmic membrane permeability, chlorophyll content, and the endogenous hormones were also tested. The results showed that the activity of POD and CAT was first increased, and then dropped with the drought time increasing. The cell membrane permeability and the contents of Pro and MDA activity were increased with the extension of treatment time. The content of chlorophyll was decreased, while the endogenous GA and the content of IAA were dropped, and the ABA content was raised. The results indicated that the protection systems of the ramie were able to protect itself from the damage of drought stress.

Key words: Drought stress, Ramie, Enzyme, Endogenous hormone, Chlorophyll, Physicochemical index

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