利用高通量测序法对模拟增温条件下藏北高寒草甸土壤线虫群落的研究

doi:10.11733/j.issn.1007-0435.2019.02.024

草地学报 ›› 2019, Vol. 27 ›› Issue (2): 443-451.DOI: 10.11733/j.issn.1007-0435.2019.02.024

利用高通量测序法对模拟增温条件下藏北高寒草甸土壤线虫群落的研究

侯磊, 薛蓓, 薛会英

西藏农牧学院, 西藏林芝 860000

收稿日期:2018-07-09 修回日期:2018-12-25 出版日期:2019-04-15 发布日期:2019-05-30
通讯作者: 薛会英
作者简介:侯磊(1988-),男,陕西宝鸡人,硕士,讲师,主要从事高原环境科学的教学与研究,E-mail:465133704@qq.com
基金资助:
国家自然科学基金（31660155）；西藏特色农牧资源研发协同创新中心高原生态项目资助

Study on Soil Nematode Community of Northern Tibetan Alpine Meadow under Simulated Warming Condition by High-throughput Sequencing Method

HOU Lei, XUE Bei, XUE Hui-ying

Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet 860000, China

Received:2018-07-09 Revised:2018-12-25 Online:2019-04-15 Published:2019-05-30

摘要/Abstract

摘要： 青藏高原对气候变化十分敏感，为探讨气候变化对藏北高寒草甸土壤线虫群落的影响，以开顶式气室（open top chamber，OTC）模拟增温3个月，利用高通量测序技术分析OTC内外不同深度土壤线虫的群落组成，探索这种方法在高原土壤线虫研究中的应用效果。结果表明，OTC内色矛纲（Chromadorea）线虫的相对丰度都较OTC外小，OTC内刺嘴纲（Enoplea）线虫都较OTC外大；OTC内矛线目（Dorylaimida）线虫的相对丰度都较OTC外大，OTC内垫刃目（Tylenchida）线虫都较OTC外小。土壤理化指标Cu²⁺、Zn²⁺、有机质和pH与纲水平土壤线虫相关性较大。短期增温已使藏北高寒草甸土壤线虫群落组成在纲、目水平上发生改变，在长期增温及大幅度增温的未来有可能发生更大的不可测变化，从而深刻影响青藏高原草地生态系统。

关键词: 增温, 高通量测序, 土壤线虫, 高寒草甸, 藏北

Abstract: The Tibet Plateau is very sensitive to climate change. In order to explore the effect of climate change on soil nematode communities of alpine meadow in northern Tibet,we used OTC (open top chamber) to simulate climate warming for 3 months,and the difference of soil nematode community at different depth between inside and outside of OTC was analyzed using MiSeq high-throughput sequencing. The results showed that the relative abundance of Chromadorea was less inside of OTC than outside of OTC,the relative abundance of Enoplea was more inside of OTC than outside of OTC;the relative abundance of Dorylaimida was more inside of OTC than outside of OTC,the relative abundance of Tylenchida was less inside of OTC than outside of OTC. The soil physical and chemical indexes,Cu²⁺,Zn²⁺,organic matter and pH,were highly correlated with soil nematode at class level. Short-term warming changed the soil nematode community of alpine meadow in the northern Tibet. Long-term and large-scale warming might occur more unpredictable changes in the future and affect the alpine grassland ecosystem.

Key words: warming, high-throughput sequencing, soil nematode, alpine meadow, northern Tibet

中图分类号:

S812.6

侯磊, 薛蓓, 薛会英. 利用高通量测序法对模拟增温条件下藏北高寒草甸土壤线虫群落的研究[J]. 草地学报, 2019, 27(2): 443-451.

HOU Lei, XUE Bei, XUE Hui-ying. Study on Soil Nematode Community of Northern Tibetan Alpine Meadow under Simulated Warming Condition by High-throughput Sequencing Method[J]. Acta Agrestia Sinica, 2019, 27(2): 443-451.

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利用高通量测序法对模拟增温条件下藏北高寒草甸土壤线虫群落的研究

Study on Soil Nematode Community of Northern Tibetan Alpine Meadow under Simulated Warming Condition by High-throughput Sequencing Method

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