高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位

doi:10.11733/j.issn.1007-0435.2021.10.005

草地学报 ›› 2021, Vol. 29 ›› Issue (10): 2149-2157.DOI: 10.11733/j.issn.1007-0435.2021.10.005

高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位

吴国芳, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇

内蒙古农业大学农学院, 内蒙古呼和浩特 010019

收稿日期:2021-04-02 修回日期:2021-06-17 出版日期:2021-10-15 发布日期:2021-11-05
通讯作者: 于肖夏,E-mail:yuxiaoxia1985@sina.com
作者简介:吴国芳(1992-),女,内蒙古凉城人,博士研究生,主要从事饲用作物遗传育种研究,E-mail:wuguofang25@163.com
基金资助:
国家自然科学基金面上项目（31772664）资助。

Fine mapping of main QTL PA7-2 for low hydrocyanic acid content in sorghum-sudangrass hybrid

WU Guo-fang, YU zhuo, YU Xiao-xia, YANG Dong-sheng, LU Qian-qian, LI Jing-wei, LI Jia-qi

Agricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China

Received:2021-04-02 Revised:2021-06-17 Online:2021-10-15 Published:2021-11-05

摘要/Abstract

摘要： 为了精细定位高丹草低氢氰酸含量性状主效数量性状基因座QTL PA7-2，对进一步开展低氰性状相关候选基因挖掘、功能解析及分子标记辅助育种等研究提供理论依据。本试验在前期研究工作基础上，从高丹草（散穗高粱Scattered ear Sorghum bicolor×红壳苏丹草Red shell Sorghum sudanense）F₂代1 200个分离群体单株中筛选出121个QIRs（Quantitative trait locus（QTL）isogenic recombinants）植株，选出低氰和高氰极端株套袋自交获得F₃代分离群体，选出QIRs群体植株130个，利用BSA-SSR（Bulked segregation analysis（BSA）and simple sequence repeats）技术构建了长度为230.7 cM、密度为4.81 cM的遗传连锁图谱，通过定位分析明确了QTL PA7-2的位置在38 cM处，位于SSR标记Sobic.8 g1-600和XM00242-400之间。经与高粱基因组比对分析，首次将低氰QTL PA7-2精细定位至高粱8号染色体3.77 Mb（51.415 Mb~55.182 Mb）的物理区间，并发现SSR标记SORBI4G3-600与其紧密连锁。

关键词: 高丹草, 低氢氰酸含量性状, 主效QTL PA7-2, BSA-SSR技术, 精细定位

Abstract: Main quantitative trait locus (QTL) PA7-2 controls low hydrocyanic acid content trait of sorghum-sudangrass hybrid, and fine mapping of this gene can provide the basis of further research on candidate genes related to low-cyanide trait, function analysis, and molecular marker-assisted breeding. Based on our previous research work, a total of 121 quantitative trait loci isogenic recombinants (QIRs) were selected from the F₂ segregation population containing 1 200 individual plants of sorghum-sudangrass hybrid (Scattered ear Sorghum bicolor×Red shell Sorghum sudanense) in this study. Plants with very low or high cyanide traits were bagged and self-crossed to obtain an F₃ segregation population, and from which 130 QIRs individual strains were screened. A genetic linkage map was constructed using bulk segregate analysis and simple sequence repeat markers (BSA-SSR), which covered a total length of 230.7 cM, with an average marker distance of 4.81 cM. The QTL PA7-2 was located at 38 cM, and between SSR markers Sobic.8 g1-600 and XM00242-400. Comparing with the sorghum genome, the low-cyanide QTL PA7-2 was finely mapped at the physical interval of 3.77 Mb (51.415 Mb-55.182 Mb) of chromosome 8 in the sorghum genome for the first time, and an SSR marker SORBI4G3-600 was closely linked with it.

Key words: Sorghum-sudangrass hybrid, Low hydrocyanic acid content trait, Major QTL PA7-2, BSA-SSR technology, Fine mapping

中图分类号:

S431.4

吴国芳, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇. 高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位[J]. 草地学报, 2021, 29(10): 2149-2157.

WU Guo-fang, YU zhuo, YU Xiao-xia, YANG Dong-sheng, LU Qian-qian, LI Jing-wei, LI Jia-qi. Fine mapping of main QTL PA7-2 for low hydrocyanic acid content in sorghum-sudangrass hybrid[J]. Acta Agrestia Sinica, 2021, 29(10): 2149-2157.

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高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位

Fine mapping of main QTL PA7-2 for low hydrocyanic acid content in sorghum-sudangrass hybrid

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