草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (1): 100-107.DOI: 10.11733/j.issn.1007-0435.2022.01.013

• 研究论文 • 上一篇    

不同硒源及浓度对偏关苜蓿种子萌发及物质转化的影响

张士敏, 朱慧森, 赵娇阳, 卫凯, 夏方山   

  1. 山西农业大学草业学院，山西 太谷 030801
  • 收稿日期:2021-06-09 修回日期:2021-06-24 发布日期:2022-01-27
  • 通讯作者: 朱慧森, E-mail: zhuhuisen@126.com
  • 作者简介:张士敏(1987-)，男，汉族，山东济宁人，博士研究生，主要从事牧草种质资源与育种方面的研究，E-mail: 1140593286@qq.com
  • 基金资助:
    国家自然科学基金(32071872、31402131)；晋中市科技重点研发计划(Y202012)资助

Effects of Different Selenium Sources and Concentrations on Seed Germination and Material Transformation of Medicago sativa 'Pianguan'

ZHANG Shi-min, ZHU Hui-sen, ZHAO Jiao-yang, WEI Kai, XIA Fang-shan   

  1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China
  • Received:2021-06-09 Revised:2021-06-24 Published:2022-01-27

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摘要： 为探究不同硒源及浓度对'偏关'苜蓿(Medicago sativa 'Pianguan')种子萌发及物质转化的影响，本研究以'偏关'苜蓿种子为材料，采用不同浓度亚硒酸钠(0，0.3，0.6，1.2，2.4，4.8，9.6 mg·L-1)及蛋氨酸硒(0，1，5，10，20，40 mg·L-1)在人工气候培养箱中进行发芽试验。结果表明：0.3 mg·L-1亚硒酸钠和1 mg·L-1蛋氨酸硒可显著促进苜蓿幼苗胚根伸长，1，5 mg·L-1蛋氨酸硒使苜蓿幼苗胚根直径显著增大。还原糖含量在亚硒酸钠及蛋氨酸硒浓度分别为0.3，0.6 mg·L-1及5，10 mg·L-1时显著增加，除4.8 mg·L-1亚硒酸钠和20，40 mg·L-1蛋氨酸硒外，硝酸还原酶活性在各硒浓度下均显著提高；0.3 mg·L-1亚硒酸钠及40 mg·L-1蛋氨酸硒可显著提高苜蓿幼苗总氨基酸含量。苜蓿种子发芽进程中对亚硒酸钠的敏感性更强，而对蛋氨酸硒的敏感性相对较弱，更有利于后期的形态建成，综合分析得出，5 mg·L-1蛋氨酸硒是苜蓿种子发芽阶段较理想的促生长剂和硒营养强化剂。

关键词: 苜蓿, 种子萌发, 物质转化, 亚硒酸钠, 蛋氨酸硒

Abstract: To explore the effects of different selenium sources and concentrations on seed germination and material transformation of Medicago sativa 'Pianguan'. This study used Medicago sativa 'Pianguan' seeds as materials, using different concentrations of sodium selenite (0, 0.3, 0.6, 1.2, 2.4, 4.8, 9.6 mg·L-1) and selenium methionine (0, 1, 5, 10, 20, 40 mg·L-1) germination test in an artificial climate incubator. The result showed: 0.3 mg·L-1 sodium selenite and 1 mg·L-1 methionine selenium significantly promoted the hypocotyl elongation of alfalfa seedlings. The hypocotyl diameter of alfalfa seedlings increased significantly under 1 and 5 mg·L-1 methionine selenium treatment. The content of reducing sugars increased significantly when the concentrations of sodium selenite and selenium methionine were 0.3, 0.6 mg·L-1 and 5, 10 mg·L-1 respectively. Except for 4.8 mg·L-1 sodium selenite and 20, 40 mg·L-1 selenium methionine, the activity of nitrate reductase can be significantly improved at each selenium concentration, 0.3 mg·L-1 sodium selenite and 40 mg·L-1 methionine selenium can significantly increase the total amino acid content of alfalfa seedlings. Alfalfa seed was more sensitive to sodium selenite during the germination process, while their sensitivity to selenium methionine was relatively weak. This is more conducive to the later morphogenesis. Comprehensive analysis shows that 5 mg·L-1 methionine selenium is an ideal growth promoter and selenium biological fortifier at the germination stage of alfalfa seeds.

Key words: Alfalfa, Seed germination, Material transformation, Sodium selenite, Methionine selenium

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