草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (9): 2336-2344.DOI: 10.11733/j.issn.1007-0435.2022.09.013

• 研究论文 • 上一篇    下一篇

不同居群红砂的遗传多样性分析

张旭, 苏世平, 师微柠, Alizet Didi Dom, 马强   

  1. 甘肃农业大学, 甘肃 兰州 730070
  • 收稿日期:2022-03-01 修回日期:2022-04-17 出版日期:2022-09-15 发布日期:2022-09-30
  • 通讯作者: 苏世平,E-mail:susp008@163.com
  • 作者简介:张旭(1996-),男,甘肃白银人,硕士研究生,主要从事林木种质资源保护研究,E-mail:1973151925@qq.com
  • 基金资助:
    国家自然科学基金项目（32060335；41661067）；甘肃省林业科技创新与国际合作资金（GLC2019-418-8）；对发展中国家常规性科技援助项目（KY202002011）；中央财政林业科技推广示范资（[2020]ZTYG15）资助

Genetic Diversity Analysis of Reaumuria soongorica Populations

ZHANG Xu, SU Shi-ping, SHI Wei-ning, Alizet Didi Dom, MA Qiang   

  1. Gansu Agricultural University, Lanzhou, Gansu Province 730070, China
  • Received:2022-03-01 Revised:2022-04-17 Online:2022-09-15 Published:2022-09-30

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摘要： 为了探究不同居群红砂（Reaumuria soogorica）的遗传多样性水平及居群遗传结构。本研究运用SSR分子标记技术对来源于内蒙古、宁夏、甘肃16个不同居群的299份红砂材料进行遗传多样性分析。结果表明11对引物共扩增出67个等位基因，扩增等位基因多态率为83.58%，多态信息含量（Polymorphism information content，PIC）为0.396；居群的等位基因数（Allele number，Na）和有效等位基因数（Effective number of alleles，Ne）为3.735，2.221；香农信息指数（Shannon information index，I）为0.763；居群间遗传分化系数（Fixation index，Fst）为0.041~0.210，平均值为0.088。AMOVA结果表明，居群间的遗传差异仅占变异总来源的5.8%。本研究得出结论红砂的基因多态性为中度，等位基因不均匀的分布在染色体上。观测杂合度和固定指数说明居群内杂合度缺失，纯合体过量。Fst指数表明居群间分化程度较低，且红砂的遗传变异主要来自源于个体内DNA序列的不同。Mantel检测得出遗传距离和地理距离显著相关。聚类分析表明居群间的基因交流是影响居群聚类的重要因素。STRUCTURE结构分析将材料分为5个亚群，第Ⅴ亚群内红砂个体血统较为纯正，第Ⅱ亚群内个体血统混杂。

关键词: 红砂, SSR, 遗传多样性, 遗传结构, 聚类分析

Abstract: In order to explore the genetic diversity level and intra-population genetic structure of different populations of Reaumuria soongorica, SSR molecular marker technology was used in this study to analyze the genetic diversity of 299 Reaumuria soongorica materials from 16 different populations in Inner Mongolia, Ningxia and Gansu. The results showed that 11 pairs of primers amplified a total of 67 alleles, the amplified allele polymorphism rate was 83.58%, and the Polymorphism Information Content (Polymorphism information content, PIC) was 0.396. The number of alleles (Allele number, Na) and Effective number of alleles (Effective number of alleles, Ne) were 3.735 and 2.221, respectively. Shannon's Information Index (Shannon information index, I) was 0.763. The coefficient of genetic differentiation among populations (F-Statistics, Fst) ranged from 0.041 to 0.210, with an average value of 0.088. AMOVA results showed that the genetic differences between populations accounted for only 5.8% of the total sources of variation. This study concluded that the gene polymorphism of Reaumuria soongorica was moderate, and the alleles were unevenly distributed on the chromosomes. The observed heterozygosity and F index indicated that the heterozygosity in the population was missing and the homozygosity was excessive. The Fst index indicated that the degree of differentiation between populations was low, and the genetic, variation of Reaumuria soongorica mainly came from the differences in DNA sequences within individuals. Mantel Test showed that genetic distance and geographic distance were significantly correlated. Cluster analysis showed that gene exchange among populations was an important factor affecting population clustering. The STRUCTURE analysis divided the materials into five subgroups. The Reaumuria soongorica individuals in The Ⅴ subgroup were relatively pure, and the individuals in the Ⅱ subgroup were mixed.

Key words: Reaumuria soongorica, SSR, Genetic diversity, Genetic structure, Cluster analysis

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