达乌里胡枝子根际解磷细菌的筛选、鉴定及特性研究

doi:10.11733/j.issn.1007-0435.2022.09.014

草地学报 ›› 2022, Vol. 30 ›› Issue (9): 2345-2355.DOI: 10.11733/j.issn.1007-0435.2022.09.014

达乌里胡枝子根际解磷细菌的筛选、鉴定及特性研究

黄臣, 杨凯元, 高鹏, 梁银萍, 韩玲娟, 赵祥

山西农业大学草业学院, 山西太谷 030801

收稿日期:2022-05-12 修回日期:2022-06-28 出版日期:2022-09-15 发布日期:2022-09-30
通讯作者: 赵祥,E-mail:sxndzhaox@126.com
作者简介:黄臣(1997-),男,汉族,安徽宣城人,硕士研究生,主要从事草地生态研究,E-mail:hc18158998185@163.com
基金资助:
国家自然科学基金（31870438）；山西农业大学科技创新基金项目（2018YJ37）；山西省2021年度研究生教育创新项目（2021Y344）资助

Screening, Identification and Characteristics of Phosphate-Solubilizing Microorganisms in Lespedeza daurica

HUANG Chen, YANG Kai-yuan, GAO Peng, LIANG Yin-ping, HAN Ling-juan, ZHAO Xiang

College of grassland science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2022-05-12 Revised:2022-06-28 Online:2022-09-15 Published:2022-09-30

摘要/Abstract

摘要： 磷匮乏是限制黄土高原地区退化草地改良和矿区生态修复中达乌里胡枝子（Lespedeza daurica）生长的重要因子。本研究采用有机磷和无机磷选择性培养基从达乌里胡枝子根际初筛获得解磷细菌，通过形态学、生理生化和分子生物学特征对目的菌株进行鉴定，利用液体发酵试验确定其解磷能力，并对其抗逆和促生特性进行研究，旨在筛选抗逆、促生的高效解磷菌株。结果表明：本研究共筛选出4株兼具解无机磷和有机磷的菌株，解磷量分别为34.96~494.03 mg·L^-1和1.93~9.15 mg·L^-1；鉴定DP24为Pseudomonas koreensis，DP25和DP27为Acinetobacter calcoaceticus，DP28为Staphylococcus epidermidis；4个菌株均可在37℃高温下生长，DP25和DP27还可在4℃低温下生长；DP28的最大耐盐量高达10%，DP28和DP25可在pH值为11~12强碱条件下生长；DP25，DP27和DP28具有分泌有机酸的能力，DP25和DP27还能分泌铁载体。总之，DP25，DP27和DP28被确定为高效、抗逆、促生解磷菌，可用作研发黄土高原地区建植达乌里胡枝子使用的菌肥资源。

关键词: 胡枝子属, 根际微生物, 解磷菌, 促生特性

Abstract: Phosphorus deficiency is an important factor limiting the growth of Lespedeza daurica in the improvement of degraded grassland and ecological restoration of mining areas in the Loess Plateau. In this study, phosphorus solubilizing bacteria were obtained from the rhizosphere of Lespedeza daurica by a selective medium of organophosphorus and inorganic phosphorus. The target strain was identified by morphological, physiological, and biochemical characteristics and molecular biological characteristics. Liquid fermentation test was used to determine the phosphorus solubilizing ability of the bacteria, and its anti-stress and growth-promoting characteristics were also studied. The aim of this study was to screen the resistant and growth-promoting strains with high efficiency phosphorus solubilization. The results showed that:Four strains were selected, and the phosphorus solubility was 34.96~494.03 mg·L^-1 and 1.93~9.15 mg·L^-1, respectively. DP24 was identified as Pseudomonas koreensis, DP25 and DP27 as Acinetobacter calcoaceticus, and DP28 as Staphylococcus epidermidis;All four strains could grow at 37℃, and DP25 and DP27 could grow at 4℃. The maximum salt tolerance of DP28 was up to 10%. DP28 and DP25 could grow under strong alkali conditions of pH11~pH12. DP25, DP27, and DP28 could secrete organic acids, and DP25 and DP27 could also secrete siderophore. In conclusion, DP25, DP27, and DP28 were identified as highly effective, anti-stress, growth-promoting, and phosphorolytic bacteria, which could be used for the development of bacterial fertilizer resources for the construction of Lespedeza daurica in the Loess Plateau.

Key words: Lespedeza, Rhizosphere microorganism, Phosphate-solubilizing microorganisms, Growth promoting characteristic

中图分类号:

S541+.5

黄臣, 杨凯元, 高鹏, 梁银萍, 韩玲娟, 赵祥. 达乌里胡枝子根际解磷细菌的筛选、鉴定及特性研究[J]. 草地学报, 2022, 30(9): 2345-2355.

HUANG Chen, YANG Kai-yuan, GAO Peng, LIANG Yin-ping, HAN Ling-juan, ZHAO Xiang. Screening, Identification and Characteristics of Phosphate-Solubilizing Microorganisms in Lespedeza daurica[J]. Acta Agrestia Sinica, 2022, 30(9): 2345-2355.

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达乌里胡枝子根际解磷细菌的筛选、鉴定及特性研究

Screening, Identification and Characteristics of Phosphate-Solubilizing Microorganisms in Lespedeza daurica

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