一种新的蒺藜苜蓿遗传杂交方法

doi:10.11733/j.issn.1007-0435.2023.03.034

草地学报 ›› 2023, Vol. 31 ›› Issue (3): 914-922.DOI: 10.11733/j.issn.1007-0435.2023.03.034

一种新的蒺藜苜蓿遗传杂交方法

张浩巍¹, 马昕巍¹, 高雅晴¹, 石文敏¹, 卢丽娟², 席杰军¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西北农林科技大学园艺学院, 陕西杨凌 712100

收稿日期:2022-10-21 修回日期:2022-11-14 出版日期:2023-03-15 发布日期:2023-04-01
通讯作者: 席杰军,E-mail:jxi@nwsuaf.edu.cn
作者简介:张浩巍(2002-),男,汉族,河南郑州人,本科生,E-mail:1186167103@qq.com;马昕巍(2001-),男,汉族,宁夏吴忠人,本科生,E-mail:1123361008@qq.com
基金资助:
国家自然科学基金（31402127）；陕西省重点产业创新链（群）-农业领域（2020ZDLNY02-05）；陕西省自然科学基金（2015JQ3079）；西北农林科技大学大学生校级科创项目（X202210712216）资助

A Novel Genetic Crossing Method for Medicago truncatula

ZHANG Hao-wei¹, MA Xin-wei¹, Gao Ya-qing¹, SHI Wen-min¹, LU Li-juan², XI Jie-jun¹

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Horticulture, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2022-10-21 Revised:2022-11-14 Online:2023-03-15 Published:2023-04-01

摘要/Abstract

摘要： 遗传杂交技术是开展遗传研究的基本工具，也是研究基因功能的必要手段之一。虽然对豆科模式植物蒺藜苜蓿（Medicago truncatula）的研究愈来愈多，但其遗传杂交技术因难度较高而只被研究蒺藜苜蓿比较悠久的科研团体掌握。为了便于更多实验室能够掌握该项技术，本文在已报道的蒺藜苜蓿杂交方法的基础上提出了一种新的杂交技术。研究内容包括蒺藜苜蓿的花果发育周期、花解剖结构、不同发育时期花的花药发育、遗传杂交程序、母本花的选择标准以及杂交鉴定等。本研究所提出的蒺藜苜蓿杂交方法与已有其它杂交方法相比，具有操作难度低、杂交后无需特殊护理措施、杂交成功率高等特点。

关键词: 豆科植物, 模式植物, 蒺藜苜蓿, 遗传杂交, 人工杂交, 花结构

Abstract: The technique of genetic crossing is a basic tool for genetic research and one of the necessary means to study gene function. Although there are more and more studies on the legume model plant Medicago truncatula, due to the difficulty of genetic hybridization of this material, its hybridization technique is still limited to the scientific research groups having utilized this plant material for a long time. In order to facilitate more laboratories to master this technology, this study proposed a new hybridization technique based on the reported hybridization methods of M. truncatula. The study included the flower and fruit development cycle, anatomical structure of flower, anther development of flowers at different stages, genetic hybridization procedures, selection criteria of female parent flowers, and identification on the successful hybridization. Compared with other existing methods of M. truncatula hybridization, the method proposed in this study featured some advantages, such as low difficulties on operation, no need for special nursing cares after hybridization, and high rate of successful hybridization.

Key words: Legumes, Model Plant, Medicago truncatula, Genetic crossing, Artificial hybridization, Anatomical structure of flower

中图分类号:

S551+.7

张浩巍, 马昕巍, 高雅晴, 石文敏, 卢丽娟, 席杰军. 一种新的蒺藜苜蓿遗传杂交方法[J]. 草地学报, 2023, 31(3): 914-922.

ZHANG Hao-wei, MA Xin-wei, Gao Ya-qing, SHI Wen-min, LU Li-juan, XI Jie-jun. A Novel Genetic Crossing Method for Medicago truncatula[J]. Acta Agrestia Sinica, 2023, 31(3): 914-922.

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一种新的蒺藜苜蓿遗传杂交方法

A Novel Genetic Crossing Method for Medicago truncatula

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