关键词: 青海草原毛虫, 转录组分析, 微卫星, 分子标记

Abstract: Gynaephora qinghaiensis is one of the important pests in the Qinghai-Tibet Plateau. In this study,transcriptome sequencing was performed on G.qinghaiensis adults and 4th instar larvae using the Illumina HiSeq^TM2000 high-throughput sequencing platform. On this basis,its microsatellite (SSR) locus was screened and the microsatellite primers were mined. 63 335 unigenes were obtained with 12 597 microsatellite loci distributed in 9 851 unigenes. Analysis of KOG,GO annotation and KEGG pathway database revealed that unigenes annotation mainly focused on general function prediction,cell progression and carbohydrate metabolism processes. SSR repeat types were mainly mononucleotide and dinucleotide repeats,(A/T) _n was the most dominant motif in mononucleotide repeats,accounting for 71.37% of the total SSR sites;(AC/GT) _n was the dominant motif of dinucleotide repeats,accounting for 6.06% of the total SSR sites. The number of SSR decreased with the number of repeats and the repeat type decreases with the length of the base sequence. Using Primer 3 software,2 714 SSR primers were designed,25 pairs were randomly selected for validation,and 11 pairs amplified the target DNA fragment. In this study,their microsatellite loci were successfully screened based on transcriptomic data,providing data support for further studies of their population genetics and occurrence dynamics.

Key words: Gynaephora qinghaiensis, Transcriptome analysis, Microsatellite loci, Molecular markers