敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析

doi:10.11733/j.issn.1007-0435.2023.11.011

草地学报 ›› 2023, Vol. 31 ›› Issue (11): 3322-3333.DOI: 10.11733/j.issn.1007-0435.2023.11.011

敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析

李硕¹, 李培英^1,2,3, 孙宗玖^1,2,3, 李雯¹

1. 新疆农业大学草业与环境科学学院, 新疆乌鲁木齐 830052;
2. 新疆草地资源与生态自治区重点实验室, 新疆乌鲁木齐 830052;
3. 西部干旱区草地资源与生态教育部重点实验室, 新疆乌鲁木齐 830052

收稿日期:2023-04-27 修回日期:2023-05-31 出版日期:2023-11-15 发布日期:2023-12-01
通讯作者: 李培英,E-mail:nmlpy_1234@sina.com
作者简介:李硕(1998-),女,汉族,内蒙古巴彦淖尔人,硕士研究生,主要从事草种资源评价与利用研究,E-mail:2438354986@qq.com
基金资助:
国家自然科学基金项目(31960362)资助

Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress

LI Shuo¹, LI Pei-ying^1,2,3, SUN Zong-jiu^1,2,3, LI Wen¹

1. College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2. Key Laboratory of Grassland Resources and Ecology of Xinjiang, Urumqi, Xinjiang 830052, China;
3. Key Laboratory of grassland resources and ecology ofwestern arid region, Urumqi, Xinjiang 830052, China

Received:2023-04-27 Revised:2023-05-31 Online:2023-11-15 Published:2023-12-01

摘要/Abstract

摘要： 狗牙根[Cynodon dactylon (Linn.) Pers]是一种优质抗旱草坪草，但其抗旱分子机制有待进一步明确。本研究以抗旱(C138)和敏旱(C32)2个狗牙根基因型为材料，对其正常灌溉(土壤相对含水量为田间持水量的80%~90%)、中度干旱(50%~60%)及重度(20%~30%)干旱处理10 d后的叶片进行取样，利用Illumina高通量测序平台进行转录组测序。与正常灌溉相比，干旱胁迫下C32有1 086个上调基因；C138有525个上调基因；C32整体响应基因数量更多，说明C32主要通过基因的正调控来响应干旱。差异基因GO富集分析显示，C138受到干旱胁迫时有更多的光合作用基因响应，C32主要富集在细胞分裂上。KEGG富集分析显示，中度胁迫下C138主要富集在叶绿素代谢和二萜类生物合成，C32主要富集在氮代谢和类黄酮生物合成；重度胁迫下C138主要富集在类黄酮生物合成，C32主要富集在氮代谢、苯并恶嗪类生物合成。中度胁迫下狗牙根抗旱可能与光合作用有关，重度胁迫下主要与次生代谢和渗透保护有关。次生代谢中的氮代谢、苯并恶嗪类生物合成和渗透保护中的谷氨酸代谢可能是狗牙根抗旱的重要富集途径。

关键词: 干旱胁迫, 狗牙根, 叶片, 转录组分析

Abstract: Bermudagrass [Cynodon dactylon (Linn.) Pers.] is an excellent drought-resistant turfgrass,and the drought-resistant molecular mechanism needs to be further clarified. In this study,the drought-resistant (C138) and drought-sensitive (C32) bermudagrass genotypes were treated under normal irrigation (soil relative water content of 80%~90% of the field water capacity),moderate drought (50%~60%) and severe (20%~30%) drought for 10 days,then the leaves were sampled for transcriptome sequencing by Illumina High-throughput sequencing platform. Compared with normal irrigation,1 086 up-regulated genes expressed for C32 under drought stress,along with 525 up-regulated for C138. The number of drought response genes in C32 was higher than C138,which indicated that C32 mainly would take a respond to drought through positive gene regulation. Differential gene GO enrichment analysis showed that C138 had more photosynthesis-related gene responded under drought stress,while C32 genes enriched on cell division. KEGG enrichment analysis showed that C138 genes mainly were enriched in chlorophyll metabolism and diterpenoid biosynthesis under moderate stress,while C32 genes mainly in nitrogen metabolism and flavonoid biosynthesis. Under severe stress,C138 was mainly enriched in flavonoid biosynthesis,while C32 mainly in nitrogen metabolism and benzoxazine biosynthesis. Drought resistance of Bermudagrass under moderate stress may be related to photosynthesis,the secondary metabolism and osmotic protection under severe stress. Nitrogen metabolism in secondary metabolism,biosynthesis of benzoxazines and glutamate metabolism in osmotic protection may be important enrichment pathways for drought resistance of bermudagrass.

Key words: Drought stress, Bermudagrass, Leaf, Transcriptome sequencing

中图分类号:

S688.4

李硕, 李培英, 孙宗玖, 李雯. 敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析[J]. 草地学报, 2023, 31(11): 3322-3333.

LI Shuo, LI Pei-ying, SUN Zong-jiu, LI Wen. Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(11): 3322-3333.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2023.11.011

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I11/3322

参考文献

[1] 韩福松,余成群,付刚,等. 低温和干旱胁迫下牧草的抗逆性机制[J]. 草地学报,2022,30(11):2856-2864
[2] ZUPIN M,SEDLAR A,KIDRIC M,et al. Drought-induced expression of aquaporin genes in leaves of two common bean cultivars differing in tolerance to drought stress[J]. Journal of Plant Research,2017,130(6):735-745
[3] 袁岐. 番茄GATA转录因子的全基因组挖掘及抗逆相关基因筛选[D]. 哈尔滨:东北农业大学,2018:42-45
[4] 胡靖康. 番茄ZF-HD转录因子的全基因组挖掘及抗逆相关基因筛选[D]. 哈尔滨:东北农业大学,2018:42-45
[5] 高莹梅. 番茄BES1转录因子的全基因组挖掘及抗逆相关基因筛选[D]. 哈尔滨:东北农业大学,2018,37-39
[6] 朱虹. 甘薯干旱转录组分析及抗旱相关基因IbWRKY2、IbGATA24和IbSDT的克隆与功能鉴定[D]. 北京:中国农业大学,2018,129-132
[7] WANG X C,YANG Z R,WANG M,et al. High-throughput sequencing technology and its application[J].China Biotechnology,2012,32(1):109-114
[8] MEYER R S,CHOI J Y,SANCHES M,et al. Domestication history and geographical adaptation inferred from a SNP map of African rice[J]. Nature Genetics,2016,48(9):2210-2227
[9] LI Y P,COLLEONI C,ZHANG J J,et al. Genomic analyses yield markers for identifying agronomically important genes in potato [J]. Molecular Plant,2018,11(3):473-484
[10] HUANG P,JIANG H,ZHU C M,et al. Sparse panicle1 is required for inflorescence development in Setaria viridis and maize[J]. Nature Plants,2017,3(5):17054
[11] 张胤冰,孙鑫博,樊波,等. 结缕草ZjNAC基因的克隆与表达分析[J]. 草业学报,2016,25(4):239-245
[12] WANG J,ZHUANG L,ZHANG J,et al. Identification and characterization of novel homeodomain leucine zipper (HD-Zip) transcription factors associated with heat tolerance in perennial ryegrass[J]. Environmental and Experimental Botany,2019,160:1-11
[13] 冷暖,刘晓巍,张娜,等. 草地早熟禾干旱胁迫转录组差异性分析[J]. 草业学报,2017,26(12):128-137
[14] 杨福良. 狗牙根草类的主要品种、特性及其建坪利用与管理技术[J]. 四川草原,2002(2):46-48
[15] 产祝龙,施海涛,王艳平. 狗牙根抗非生物胁迫的研究进展[J]. 草业科学,2013,30(8):1182-1187
[16] SHI H T,WANG Y P,CHENG Z M,et al. Analysis of natural variation in bermudagrass (Cynodon dactylon) reveals physiological responses underlying drought tolerance[J]. PLoS One,2012,7(12):522-534
[17] LU S,CHEN C,WANG Z,et al. Physiological responses of somaclonal variants of triploid bermudagrass (Cynodon transvaalensis x Cynodon dactylon) to drought stress[J]. Plant Cell Reports,2009,28(3):517-526.
[18] ZHOU P,AN Y,WANG Z,et al. Characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species[J]. Plos One,2014,9(8):103-116
[19] LV A,FAN N,XIE J,et al. Expression of CdDHN4,a novel YSK2-type dehydrin gene from bermudagrass,responses to drought stress through the ABA-dependent signal pathway[J]. Frontiers in Plant Science,2017,16(8):748-762
[20] CHEN M,ZHAO Y,ZHUO C,et al. Overexpression of a NF-YC transcription factor from bermudagrass confers tolerance to drought and salinity in transgenic rice[J]. Plant Biotechnology Journal,2015,13(4):482-491
[21] 曾令霜,李培英,孙晓梵,等. 新疆不同生境狗牙根种质抗旱性综合评价[J].草业学报,2020,29(8):155-169
[22] 曾令霜,李培英,孙宗玖,等. 两类新疆狗牙根抗旱基因型抗氧化酶保护系统及其基因表达差异分析[J]. 草业学报,2022,31(7):122-132
[23] FRACASSO A,TRINDADE L,AMADUCCI S. Drought tolerance strategies highlighted by two sorghum bicolor races in a dry-down experiment [J]. Journal of Plant Physiology,2016,190(12):1-14
[24] KIM C,LEMKE C,PATERSON A H. Functional dissection of drought-responsive gene expression patterns in Cynodon dactylon L[J]. Plant Molecular Biology,2009,70(2):1-16
[25] 张然,马祥,朱瑞婷,等. 青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析[J]. 草地学报,2020,28(6):1508-1518
[26] 严平,韦朝领,蒋跃林,等. 土壤水分对小麦光合作用影响的研究[J]. 作物杂志,2000(1):13-14
[27] BOYER J,RAO I. Progress in Photosynthesis Research[M]. Dordrecht:Springer Dordrecht,1986:147-151
[28] 曹慧,兰彦平,刘会超,等. 水分胁迫下短枝型苹果幼树活性氧代谢失调对光合作用的影响[J].内蒙古农业大学学报(自然科学版),2000(3):22-25
[29] 陈培元. 作物对干旱逆境的适应性和反应[J].山西农业科学,1990(9):29-32,7
[30] 张一龙,孙晓梵,李硕,等. 不同抗旱性狗牙根种质的抗旱生理响应差异分析[J]. 中国农业科技导报,2023,25(6):59-70
[31] HU L,WANG Z,HUANG B. Diffusion limitations and metabolic factors associated with inhibition and recovery of photosynthesis from drought stress in a C perennial grass species[J]. Physiologia Plantarum,2010,139(1):93-106
[32] 肖列,刘国彬,李鹏,等. 白羊草光合特性及非结构性碳水化合物含量对CO₂浓度倍增和干旱胁迫的响应[J]. 植物营养与肥料学报,2017,23(2):389-397
[33] HU L,WANG Z,HUANG B. Photosynthetic responses of bermudagrass to drought stress associated with stomatal and metabolic limitations[J]. Crop Science,2009,49(5):1902-1909
[34] 张一龙,喻启坤,李雯,等. 不同抗旱性狗牙根地上地下表型特征及内源激素对干旱胁迫的响应[J]. 草业学报,2023,32(3):163-178
[35] CHEN L,FAN J,HU L,et al. A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance [J]. BMC Plant Biology,2015,15(1):216
[36] XU Z Z,ZHOU G S. Nitrogen Metabolism and Photosynthesis in Leymus chinensis in response to long-term soil drought [J]. Journal of Plant Growth Regulation,2006,25(3):252-266
[37] BRINI F,HANIN M,LUMBRERAS V,et al. Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana [J]. Plant Cell Reports,2007,26(11):2017-2026
[38] 王玉斌,牛皓,吕鑫,等. 旱敏感型与耐旱型高粱材料对干旱胁迫反应的转录组差异分析[J]. 分子植物育种,2022,20(20):6656-6667
[39] 李春艳,董洁,钟华,等. 白羊草叶片和根系干旱胁迫下microRNAs差异表达分析[J]. 草地学报,2019,27(3):539-546
[40] ZHU H,YU X,XU T,et al. Transcriptome profiling of cold acclimation in bermudagrass (Cynodon dactylon)[J]. Scientia Horticulturae,2015,66(3):681-649
[41] MATSUKURA S,MIZOI J,YOSHIDA T,et al. Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes[J]. Molecular Genetics and Genomics,2010,283(2):185-196
[42] 吴婧,范菠菠,张学峰,等. 蒙古冰草ERF转录因子生物信息学及其表达分析[J]. 草地学报,2022,30(11):2910-2921

敏旱和抗旱狗牙根叶片对干旱胁迫的转录组响应差异分析

Analysis of the Difference in Transcriptome Responses of Drought-Sensitive and Drought-Tolerant Leaves of Bermudagrass to Drought Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	蒋宇佳, 于元平, 孙向一, 周敏, 吴春妍, 李玉华, 刘明稀. 假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2628-2641.
[2]	南彦斌, 许嘉诚, 何啟玥, 潘学能, 周渊涛. 青海草原毛虫转录组分析及SSR位点开发[J]. 草地学报, 2023, 31(9): 2653-2662.
[3]	冯树林, 周婷, 王军利. 胡枝子幼苗不同生长阶段叶水势对干旱-复水的响应特征[J]. 草地学报, 2023, 31(7): 2077-2085.
[4]	于思敏, 罗永忠, 康芳明, 邱应德, 李转桃. 干旱胁迫对紫花苜蓿生长和叶绿素荧光特性的影响[J]. 草地学报, 2023, 31(6): 1762-1771.
[5]	岑慧芳, 钱文武, 朱慧森, 夏方山, 杜利霞, 许涛. 干旱胁迫对草地早熟禾叶片显微结构和光合特征的影响[J]. 草地学报, 2023, 31(5): 1368-1377.
[6]	黄荣雁, 孟维珊, 朱琨, 刘殿辉, 赵竹婷, 路程伟, 孙正轩, 唐宽宇, 周佳奇, 程瑜. 细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应[J]. 草地学报, 2023, 31(5): 1386-1392.
[7]	任明辉, 张雨蓬, 许涛, 朱慧森, 岑慧芳. 紫花苜蓿R2R3-MYB亚家族鉴定与干旱胁迫下的表达分析[J]. 草地学报, 2023, 31(4): 972-983.
[8]	吴学蕤, 赵庆霞, 蔡银美, 张成富, 李官亮. 干旱-复水对构树叶片水势和气孔开闭的影响[J]. 草地学报, 2023, 31(3): 769-776.
[9]	师微柠, 苏世平, 李毅, 张䶮, 席杰. 干旱生境下外源脯氨酸对红砂气孔形态的影响[J]. 草地学报, 2023, 31(3): 777-784.
[10]	马小兰, 周华坤, 张正芳, 李珍珍, 徐梦真, 黄奥伟, 王文颖, 殷恒霞. 外源IAA对干旱胁迫下红豆草种子萌发及幼苗生长的影响[J]. 草地学报, 2023, 31(3): 796-803.
[11]	李双铭, 杨勇, 胡龙兴, 徐庆国. 不同纬度来源结缕草叶片响应低温胁迫的转录组分析[J]. 草地学报, 2023, 31(10): 2968-2984.
[12]	苏原, 周家如, 王亭帅, 杨倩雯, 李东旭, 武帅楷, 刁华杰, 王常慧, 董宽虎. 氮添加降低盐渍化草地赖草非结构性碳水化合物含量[J]. 草地学报, 2023, 31(10): 3000-3006.
[13]	肖晴, 林轸荣, 姜风岩, 石丽娜, 赵洪鑫, 李永元, 祁进贤, 党志英, 董全民, 周华坤, 邵新庆. 放牧强度对高寒草甸优势植物叶片解剖结构的影响[J]. 草地学报, 2023, 31(10): 3018-3025.
[14]	李春艳, 钟华, 杜利霞, 侯向阳. 白羊草BiMYB52基因的克隆及转基因拟南芥抗旱性表达分析[J]. 草地学报, 2023, 31(1): 29-39.
[15]	苟甜甜, 马艳娜, 吉轶楠, 臧国长, 郑轶琦. 狗牙根小尺度克隆多样性及空间遗传结构研究[J]. 草地学报, 2023, 31(1): 81-88.