直立型半野生大豆叶绿体基因组分析

doi:10.11733/j.issn.1007-0435.2023.11.012

草地学报 ›› 2023, Vol. 31 ›› Issue (11): 3334-3342.DOI: 10.11733/j.issn.1007-0435.2023.11.012

直立型半野生大豆叶绿体基因组分析

郭冉昊^1,2, 赵淑文^1,2, 米福贵¹, 候伟峰³, 赵力兴³

1. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010000;
2. 内蒙古农业大学植物学国家级实验教学示范中心, 内蒙古呼和浩特 010000;
3. 兴安盟农牧科学研究所, 内蒙古兴安盟 137400

收稿日期:2023-04-18 修回日期:2023-05-31 出版日期:2023-11-15 发布日期:2023-12-01
通讯作者: 赵淑文,E-mail:zhaoshuwen1226@163.com;米福贵,E-mail:mfgui@163.com
作者简介:郭冉昊(1997-),女,蒙古族,内蒙古巴彦淖尔人,主要从事牧草种质资源与利用方面研究,硕士研究生,E-mail:214597507@qq.com
基金资助:
兴安盟野生植物资源调查研究资助

Chloroplast Genome Analysis of An Erect Semi-Wild Soybean

GUO Ran-hao^1,2, ZHAO SHU-wen^1,2, MI FU-gui¹, HOU WEI-feng³, ZHAO LI-xing³

1. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010000, China;
2. National Experimental Teaching Demonstration Center of Botany, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010000, China;
3. Hinggan League Institute of Agricultural and Animal Husbandry Sciences, Hinggan League, Inner Mongolia 137400, China

Received:2023-04-18 Revised:2023-05-31 Online:2023-11-15 Published:2023-12-01

摘要/Abstract

摘要： 采用Illumina高通量测序技术对直立型半野生大豆(Glycine gracilis)叶绿体基因组(Chloroplast DNA，cpDNA)进行测序和组装，获得完整基因组，以探究其结构特征，为后续基于cpDNA的相关分析以及大豆资源多样性保护和系统发育等研究提供基础或依据。结果表明，直立型半野生大豆cpDNA全长148 320 bp，为典型四分体结构，注释基因108个，包含66个蛋白编码基因、29个tRNA基因和4个rRNA基因。基因组序列上共检测到87个SSR位点，其中与单核苷酸、双核苷酸、三核苷酸、四核苷酸、五核苷酸对应的位点分别为55，19，4，7，2个。在系统发育上，直立型半野生大豆与已公布的普通型一年生半野生大豆(Glycine gracilis)、栽培大豆(Glycine max)及野大豆(Glycine soja)亲缘关系较近。

关键词: 半野生大豆, cpDNA, 简单重复序列, 密码子偏好性, 系统发育

Abstract: Illumina high-throughput sequencing technology was used to sequence and assemble the chloroplast genome of an erect semi-wild soybean (Glycine gracilis) to obtain a complete chloroplast genome,so as to explore its structural characteristics,and provide a basis for subsequent related analysis on chloroplast genome and diversity protection and phylogeny of soybean resources. The results demonstrated that the full-length chloroplast genome of that erect semi-wild soybean was 148 320 bp,which was a typical tetrad structure.108 genes were annotated,including 66 protein-coding genes,29 tRNA genes and 4 rRNA genes. A total of 87 SSR loci were detected in the genome sequence,of which 55,19,4,7 and 2 loci were corresponding to mononucleotide,dinucleotide,trinucleotide,tetranucleotide and pentanucleotide,respectively. Based upon the phylogeny analysis,that erect semi-wild soybean was closely related to the published common annual semi-wild soybean (Glycine gracilis),cultivated soybean (Glycine max),and wild soybean (Glycine soja).

Key words: Wild soybean, Chloroplast genome, Simple repeat sequence, Codon preference, Phylogeny analysis

中图分类号:

S565.1

郭冉昊, 赵淑文, 米福贵, 候伟峰, 赵力兴. 直立型半野生大豆叶绿体基因组分析[J]. 草地学报, 2023, 31(11): 3334-3342.

GUO Ran-hao, ZHAO SHU-wen, MI FU-gui, HOU WEI-feng, ZHAO LI-xing. Chloroplast Genome Analysis of An Erect Semi-Wild Soybean[J]. Acta Agrestia Sinica, 2023, 31(11): 3334-3342.

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直立型半野生大豆叶绿体基因组分析

Chloroplast Genome Analysis of An Erect Semi-Wild Soybean

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