耐辐射甲基杆菌JB18对菊苣幼苗生长及抗性的影响

doi:10.11733/j.issn.1007-0435.2024.08.017

草地学报 ›› 2024, Vol. 32 ›› Issue (8): 2505-2513.DOI: 10.11733/j.issn.1007-0435.2024.08.017

• 研究论文 • 上一篇

耐辐射甲基杆菌JB18对菊苣幼苗生长及抗性的影响

金忠民^1,2, 刘宇恒¹, 苑新悦¹, 刘博¹, 李春月¹, 刘丽杰^1,2, 秦祥宇^1,2, 于保刚^1,2, 李铁^1,2

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006

收稿日期:2024-01-23 修回日期:2024-03-07 发布日期:2024-09-07
作者简介:金忠民(1968-),女,汉族,黑龙江绥化人,博士,教授,主要从事植物逆境生理与分子生物学研究,E-mail:yyy6768@163.com
基金资助:
黑龙江省省属高等学校基本科研业务费科研项目(NO.145309523)资助

Effects of Methylobacterium radiotolerans JB18 on the Growth and Resistance of Cichorium intybus L Seedlings

JIN Zhong-min^1,2, LIU Yu-heng¹, YUAN Xin-yue¹, LIU Bo¹, LI Chun-yue¹, LIU Li-jie^1,2, QIN Xiang-yu^1,2, YU Bao-gang^1,2, LI Tie^1,2

1. College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Heilongjiang Provincial Key Laboratory of Resistance Genetic Engineering and Coldland Biodiversity Conservation, Qiqihar, Heilongjiang Province 161006, China

Received:2024-01-23 Revised:2024-03-07 Published:2024-09-07

摘要/Abstract

摘要： 为探究铅镉抗性菌株JB18对菊苣(Cichorium intybus L)幼苗的影响，在不同铅镉复合浓度胁迫土壤中种植菊苣。将菌株JB18接种到菊苣根部周围，测定菊苣幼苗部分生长指标、光合作用参数、抗氧化酶活性、蔗糖含量、蔗糖代谢酶的活性及基因表达量。结果表明：接种菌株JB18后，提高了菊苣幼苗含水量、抗坏血酸含量、叶绿素含量、胞间CO₂浓度、蒸腾速率、净光合速率、过氧化物酶和超氧化物歧化酶活性，显著减少菊苣内丙二醛及铅镉含量。蔗糖合酶、蔗糖磷酸合酶、蔗糖转化酶活性显著提升，菌株JB18促进了叶片中蔗糖积累。RT-qPCR结果显示，受Pb-Cd胁迫并接种JB18的菊苣幼苗中SS和SPS基因的相对表达量与对照组相比，分别上调2.1倍和5.6倍。因此，菌株JB18能够提高受铅镉复合胁迫下菊苣幼苗植株光合作用速率和抗氧化能力，上调蔗糖代谢相关基因的表达，促进蔗糖的积累，提高菊苣幼苗的铅镉抗性。

关键词: 菊苣, 铅镉抗性, 菌株JB18

Abstract: To investigate the effects of Pb-Cd-resistant strain JB18 on chicory seedlings,chicory was planted in different Pb-Cd composite concentration-stressed soils. Strain JB18 was inoculated around the roots of chicory,photosynthesis parameters,antioxidant enzyme activities,sucrose content,activities of enzymes involved in sucrose metabolism,and expression of related genes in chicory seedlings were determined. The results showed that inoculation with strain JB18 increased water content,ascorbic acid content,chlorophyll content,intercellular CO₂ concentration,transpiration rate,net photosynthetic rate,peroxidase and superoxide dismutase activities,and significantly reduced malondialdehyde and Pb-Cd content in chicory seedlings. Sucrose synthase,sucrose phosphate synthase and sucrose converting enzyme activities significantly increased,and strain JB18 promoted sucrose accumulation in leaves. RT-qPCR results showed that the relative expression of SS and SPS genes in chicory seedlings subjected to Pb-Cd stress and inoculated with JB18 was up-regulated by 2.1-fold and 5.6-fold,respectively,compared with the control group. Therefore,strain JB18 was able to increase the photosynthesis rate and antioxidant capacity,up-regulate the expression of genes related to sucrose metabolism,promote the accumulation of sucrose,and improve the Pb-Cd resistance of chicory seedlings subjected to Pb-Cd compound stress.

Key words: Chicory；Pb-Cd, resistance；Strain, JB18

中图分类号:

金忠民, 刘宇恒, 苑新悦, 刘博, 李春月, 刘丽杰, 秦祥宇, 于保刚, 李铁. 耐辐射甲基杆菌JB18对菊苣幼苗生长及抗性的影响[J]. 草地学报, 2024, 32(8): 2505-2513.

JIN Zhong-min, LIU Yu-heng, YUAN Xin-yue, LIU Bo, LI Chun-yue, LIU Li-jie, QIN Xiang-yu, YU Bao-gang, LI Tie. Effects of Methylobacterium radiotolerans JB18 on the Growth and Resistance of Cichorium intybus L Seedlings[J]. Acta Agrestia Sinica, 2024, 32(8): 2505-2513.

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参考文献

[1] PATHAK A,SINGH S P,TRIPATHI A M,et al. Cichorium intybus:A review on its medicinal importance[J]. Journal of Veterinary Pharmacology and Toxicology,2022,21(1):1-8
[2] WAN X H,GUO H,LIANG Y Y.et al. The physiological functions and pharmaceutical applicationsof inulin:A review[J]. Carbohydrate Polymers,2020,246(20):116589
[3] GHOLAMI H,FARD F R,SAHARKHIZ M J,et al. Yield and physicochemical properties of inulin obtained from Iranian chicory roots under vermicompost and humic acid treatments[J]. Industrial Crops and Products,2018,123:610-616
[4] PEROVIC' J,SˇAPONJAC V T,KOJIC' J,et al. Chicory(Cichorium intybus L.)as a food ingredient-Nutritional composition,bioactivity,safety,and health claims:A review[J]. Food chemistry,2021,336:127676
[5] MOSCATELLO S,BATTISTELLI A,MATTIONI M,et al. Yield,Fructans Accumulation,and Nutritional Quality of Young Chicory Plants as Related to Genotype and Nitrogen Fertilization[J]. Agronomy,2023,13(7):1752
[6] BQYE H,HYMETE A. Levels of heavy metals in common medicinal plants collected from environmentally different sites[J]. Middle East Journal of Scientific Research,2013,13(7):938
[7] 徐梦琪,杨文弢,杨利玉,等. 黔西北山区耕地重金属健康风险评价及环境基准[J]. 环境科学,2022,43(7):3799-3810
[8] ATUCHIN V V,ASYAKINA L K,SERAZETDINOVA Y R,et al. Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals[J]. Microorganisms,2023,11(4):864
[9] SUN L J,GONG P,SUN Y F,et al. Modified chicken manure biochar enhanced the adsorption for Cd²⁺ in aqueous and immobilization of Cd in contaminated agricultural soil[J]. Science of the Total Environment,2022(851):158252
[10] PRIYADARSHANEE M,DAS S. Biosorption and removal of toxic heavy metals by metal tolerating bacteria for bioremediation of metal contamination:A comprehensive review[J]. Journal of Environmental Chemical Engineering,2021,9(1):104686
[11] MALIK B,PIRZADAH T B,TAHIR I,et al. Lead and aluminium-induced oxidative stress and alteration in the activities of antioxidant enzymes in chicory plants[J]. Scientia Horticulturae,2021(278):109847
[12] 姚智卿.铅对人体健康的危害[J]. 微量元素与健康研究,2011,28(5):67-68
[13] SUN L J,GONG P,SUN Y F,et al. Modified chicken manure biochar enhanced the adsorption for Cd²⁺ in aqueous and immobilization of Cd in contaminated agricultural soil[J]. Science of the Total Environment,2022,851:158252
[14] IORI V,GAUDET M,FABBRINI F,et al. Physiology and genetic architecture of traits associated with cadmium tolerance and accumulation in Populus nigra L[J]. Trees,2016(30):125-139
[15] YUAN W Z,YANG N,LI X K. Advances in understanding how heavy metal pollution triggers gastric cancer[J]. BioMed research International,2016(10):7825432
[16] MATHIVANAN K,CHANDIRIKA J U,MATHIMANI T,et al. Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment[J]. Ecotoxicology and Environmental Safety,2021,208:111567
[17] DAS S,LYLA P S,KHAN S A.Marine microbial diversity and ecology:importance and future perspectives[J].Current Science,2006:1325-1335
[18] MA B,SONG W,ZHANG X,et al. Potential application of novel cadmium-tolerant bacteria in bioremediation of Cd-contaminated soil[J]. Ecotoxicology and Environmental Safety,2023,255:114766
[19] ARIf M.S,YASMEEN T,SHAHZAD S M,et al. Lead toxicity induced phytotoxic effects on mung bean can be relegated by lead tolerant Bacillus subtilis (PbRB3)[J]. Chemosphere,2019,234:70-80
[20] 杨怡森,孙晨瑜,马俊卿,等. 玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应[J]. 生态与农村环境学报,2023,39(10):1316-1322
[21] 苍晶.植物生理生化实验原理与技术[M]. 北京:高等教育出版社,2013:148-151
[22] FAZAL H,TARIQ A. A mini review on lead(Pb)toxicity in plants[J]. Journal of Biology and Life Science,2015,6(2):91-101
[23] 曾秀存,许耀照,张芬琴,等. 两种基因型龙葵对镉胁迫的生理响应及镉吸收差异[J]. 农业环境科学学报,2012,31(5):885-890
[24] PHOTOLO M M,SITOLE L,MAVUMENGWANA V,et al. Genomic and physiological investigation of heavy metal resistance from plant endophytic Methylobacterium radiotolerans MAMP 4754,isolated from Combretum erythrophyllum[J]. International Journal of Environmental Research and Public Health,2021,18(3):997-1009
[25] PROMBUNCHACHAI T,NAKAEW N,CHIDBUREE A,et al. Effect of Methylobacterium radiotolerans ED5-9 with capability of producing indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid deaminase on the growth and development of Murdannia loriformis (Hassk.) Rolla Rao & Kammathy under in vitro condition[J]. Naresuan University Journal:Science and Technology，2017,25(2):21-31
[26] SABIR A,NAVEED M,BASHIR M A,et al. Cadmium mediated phytotoxic impacts in Brassica napus:Managing growth,physiological and oxidative disturbances through combined use of biochar and Enterobacter sp. MN17[J]. Journal of Environmental Management,2020,265:110522
[27] 王建秋,曹子林,王晓丽,等.铅胁迫对滇白前生长、光合作用及叶绿素荧光的影响[J]. 草地学报,2021,29(11):2422-2427
[28] 邹文桐,王艳君,曹智,等. 不同种植模式下重金属污染对牧草叶片光合特性和重金属含量的影响[J]. 热带亚热带植物学报,2021,29(1):31-40
[29] ANKET S,VINOD K,BABAR S,et al. Photosynthetic response of plants under different abiotic stresses:A review[J]. Journal of Plant Growth Regulation,2019,39(2):509-531
[30] HEYNO E,KLOSE C,KRIEGER-LISZKAY A.Origin of cadmium-induced reactive oxygen species production:mitochondrial electron transfer versus plasma membrane NADPH oxidase[J].New Phytologist,2008,179(3):687-699
[31] 杜雪,彭玉兰,张新彤,等. 重金属铜、锌对火炬树幼苗生长及生理指标的影响[J]. 西南农业学报,2022,35(6):1407-1414
[32] 金忠民,李春月,刘本松,等. 菌株JB12影响铅镉胁迫下菊苣黄酮合成的转录组分析[J]. 草地学报,2023,31(06):1648-1655
[33] QIN S,LIU H,NIE Z,et al. AsA-GSH cycle and antioxidant enzymes play important roles in Cd tolerance of wheat[J]. Bulletin of Environmental Contamination and Toxicology,2018,101(5):684-690
[34] KHANNA K,JAMWAL V L,KOHLI S K,et al. Plant growth promoting rhizobacteria induced Cd tolerance in Lycopersicon esculentum through altered antioxidative defence expression[J]. Chemosphere,2019,217:463-474
[35] NAVEED M,MUSTAFA A,AZHAR S Q T A,et al. Burkholderia phytofirmans PsJN and tree twigs derived biochar together retrieved Pb-induced growth,physiological and biochemical disturbances by minimizing its uptake and translocation in mung bean (Vigna radiata L.)[J]. Journal of Environmental Management,2020,257:109974
[36] JIN Z M,DENG S Q,WEN Y,et al. Application of Simplicillium chinense for Cd and Pb biosorption and enhancing heavy metal phytoremediation of soils[J]. Science of the Total Environment,2019,697:134148
[37] HAN H,SHENG X,HU J,et al. Metal-immobilizing Serratia liquefaciens CL-1 and Bacillus thuringiensis X30 increase biomass and reduce heavy metal accumulation of radish under field conditions[J]. Ecotoxicology and Environmental Safety,2018,161:526-533
[38] 高慧兵,郁培义,孙宇靖,等. 铅锌胁迫下蓖麻叶糖代谢规律及相关酶基因差异表达分析[J]. 植物生理学报,2019,55(4):483-492
[39] DOEHLERT D C,HUBER S C. Regulation of spinach leaf sucrose phosphate synthase by glucosephosphate,inorganic phosphate,and pH[J]. Plant Physiol,1983,73(4):989-994
[40] KATHARINA S,ALEXANDER G,MARGO D,et al. Sucrose synthase:A unique glycosyltransferase for biocatalytic glycosylation process development[J]. Biotechnology Advances,2016,34(2):88-111
[41] SHEN J,XU Y,YUAN S,et al. Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress[J]. International Journal of Molecular Sciences,2023,24(16):12878
[42] 王日明,王志强,向佐湘,等. γ-氨基丁酸对高温胁迫下黑麦草光合特性及碳水化合物代谢的影响[J]. 草业学报,2019,28(2):168-178
[43] KAZEROONI E A,MAHARACHCHIKUMBURA S S N,ALSADI A M,et al. Effects of the rhizosphere fungus cunninghamella bertholletiae on the Solanum lycopersicum response to diverse abiotic stresses[J]. International Journal of Molecular Sciences,2022,23(16):8909(责任编辑刘婷婷)第32卷第8期 Vol.32 No. 8草地学报 ACTAAGRESTIASINICA 2024年 8月

耐辐射甲基杆菌JB18对菊苣幼苗生长及抗性的影响

Effects of Methylobacterium radiotolerans JB18 on the Growth and Resistance of Cichorium intybus L Seedlings

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