‘宁杂4号’美洲狼尾草×‘紫色’象草三倍体杂交种的创制与高产高花青素优系筛选

doi:10.11733/j.issn.1007-0435.2026.08.011

草地学报 ›› 2026, Vol. 34 ›› Issue (8): 2866-2876.DOI: 10.11733/j.issn.1007-0435.2026.08.011

• 研究论文 • 上一篇

‘宁杂4号’美洲狼尾草×‘紫色’象草三倍体杂交种的创制与高产高花青素优系筛选

王鑫, 肖卓琳, 卫尊征, 滕珂, 穆娜, 周仂, 岳跃森, 范希峰

北京市农林科学院草业花卉与景观生态研究所, 北京 100097

收稿日期:2026-02-05 修回日期:2026-03-07 发布日期:2026-08-04
通讯作者: 范希峰,E-mail:fanxifeng@baafs.net.cn
作者简介:王鑫(2000-),男,汉族,重庆潼南人,博士研究生,主要从事饲草遗传育种研究,E-mail:3217687484@qq.com

Creation of a Triploid Hybrid of Pennisetum glaucum ‘Ningza No.4’×P. purpureum ‘purple’ and Screening for High-yielding， High-antocyanin Superior Lines

WANG Xin, XIAO Zhuo-lin, WEI Zun-zheng, TENG Ke, MU Na, ZHOU Le, YUE Yue-sen, FAN Xi-feng

Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2026-02-05 Revised:2026-03-07 Published:2026-08-04

摘要/Abstract

摘要： 为获得高产优质、富含花青素的饲草新种质，本研究以二倍体‘宁杂4号’美洲狼尾草（Pennisetum glaucum‘Ningza No.4’）做母本，四倍体‘紫色’象草（P. purpureum‘purple’）做父本开展种间杂交。杂交F₁种子萌发和成苗率以及真实性鉴定表明，‘宁杂4号’美洲狼尾草与‘紫色’象草尽管存在一定杂交后障碍，但仍可产生杂交后代；且证实，因亲本DNA含量相似，流式细胞检测技术不适合杂交后代真实性鉴定，而结合染色体计数与分子标记两种方法更为准确有效。进一步结合亲本及杂交子代的农艺性状和营养品质测定结果表明，亲本与杂交子代表现出明显差异，部分子代单株鲜重较父本提升46%，花青素含量提升27%。经筛选获得多个优系单株并进行了扩繁。第二年测定的数据表明，杂交子代优系在鲜草产量、干草产量、花青素含量方面皆优于亲本。本研究筛选出的三倍体杂交优系在产量和花青素含量方面表现优异，为后续饲草新品种选育提供了重要材料。

关键词: 美洲狼尾草, ‘紫色’象草, 杂交育种, 饲草新种质

Abstract: To obtain high-yielding， high-quality， and anthocyanin-rich new germplasm of forage grass， in this study we utilized a diploid Pennisetum glaucum ‘Ningza No.4’ as the maternal parent and a tetraploid P. purpureum ‘purple’ as the paternal parent to conduct interspecific hybridization. The germination and seedling establishment rates of the hybrid F₁ seeds， along with authenticity verification， indicated that despite certain hybridization barriers between P. glaucum ‘Ningza No.4’ and P. purpureum ‘purple’， hybrid offspring could still be produced. Furthermore， it was confirmed that due to the similarity in DNA content between the parental lines， flow cytometry was not suitable for authenticity verification of the hybrid offspring； instead， a combination of chromosome counting and molecular marker methods proved to be more accurate and effective. Further integration of agronomic traits and nutritional quality measurements of parents and their hybrid offspring showed that parents and hybrid offspring exhibited significant differences. Some individual hybrid plants had a 46% higher fresh weight than their paternal parent and a 27% higher anthocyanin content. Multiple superior individual plants were selected and propagated. Data collected in the second year indicated that the superior hybrid lines outperformed the parental lines in terms of fresh forage yield， dry forage yield， and anthocyanin content. The triploid hybrid superior strain selected in this study showed excellent performance in terms of yield and anthocyanin content， providing important materials for the subsequent breeding of new forage varieties.

Key words: Pennisetum glaucum, Pennisetum purpureum ‘purple’, Crossbreeding, New forage germplasm

中图分类号:

S543.9

王鑫, 肖卓琳, 卫尊征, 滕珂, 穆娜, 周仂, 岳跃森, 范希峰. ‘宁杂4号’美洲狼尾草×‘紫色’象草三倍体杂交种的创制与高产高花青素优系筛选[J]. 草地学报, 2026, 34(8): 2866-2876.

WANG Xin, XIAO Zhuo-lin, WEI Zun-zheng, TENG Ke, MU Na, ZHOU Le, YUE Yue-sen, FAN Xi-feng. Creation of a Triploid Hybrid of Pennisetum glaucum ‘Ningza No.4’×P. purpureum ‘purple’ and Screening for High-yielding， High-antocyanin Superior Lines[J]. Acta Agrestia Sinica, 2026, 34(8): 2866-2876.

参考文献

[1] DADUWAL H S，BHARDWAJ R，SRIVASTAVA R K. Pearl millet a promising fodder crop for changing climate： A review[J]. Theoretical and Applied Genetics，2024，137：169
[2] DOWLING C D. Marker-assisted verification of hybrids in pearl millet-napiergrass （Pennisetum glaucum （L.） BrR..×Pennisetum purpureum Schumach.）[D]. College Station，Texas：Texas A & M University，2012：1-3
[3] MALEKO D，NG W T，MSALYA G，et al. Seasonal variations in the availability of fodder resources and practices of dairy cattle feeding among the smallholder farmers in Western Usambara Highlands， Tanzania[J]. Tropical Animal Health and Production，2018，50：1653-1664
[4] MTENGETI E J，PHIRI E C J H，URIO N A，et al. Forage availability and its quality in the dry season on smallholder dairy farms in Tanzania[J]. Acta Agriculturae Scandinavica Section A，2008，58（4）：196-204
[5] HOU X C，TENG K，GUO Q，et al. Research advances in forage Pennisetum resource[J]. Chinese Bulletin of Botany，2022，57（6）：814-825侯新村，滕珂，郭强，等. 狼尾草属牧草研究进展[J]. 植物学报，2022，57（6）：814-825
[6] KELLOGG E A. The grasses： a case study in macroevolution[J]. Annual Review of Ecology and Systematics，2000，31（1）：217-238
[7] OBOK E E，AKEN'OVA M E，IWO G A. Forage potentials of interspecific hybrids between elephant grass selections and cultivated pearl millet genotypes of Nigerian origin[J]. Journal of Plant Breeding and Crop Science，2012，4（9）：136-143
[8] SERBA D D，PERUMAL R，TESSO T T，et al. Status of global pearl millet breeding programs and the way forward[J]. Crop Science，2017，57（6）：2891-2905
[9] MUKTAR M S，HABTE E，TESHOME A，et al. Insights into the genetic architecture of complex traits in napier grass （Cenchrus purpureus） and QTL regions governing forage biomass yield， water use efficiency and feed quality traits[J]. Frontiers in Plant Science，2022，12：678862
[10] NEGAWO A T，TESHOME A，KUMAR A，et al. Opportunities for napier grass （Pennisetum purpureum） improvement using molecular genetics[J]. Agronomy，2017，7（2）：28
[11] LIU G H，YANG S Q，LIU M J，et al. Analysis of genome size and leaf heterosis of hybrids between diploid and tetraploid in Chinese jujube[J]. China Fruits，2025（1）：49-54刘冠豪，杨松颀，刘孟军，等. 枣二、四倍体间杂交子代基因组大小及叶片性状变异分析[J]. 中国果树，2025（1）：49-54
[12] WANG Z J，TANG C M，DAI F W，et al. Inheritance characteristics of leaf quality traits of different ploidy mulberry varieties’grossed progenies[J]. Chinese Agricultural Science Bulletin， 2018，34（15）：85-88王振江，唐翠明，戴凡炜，等. 不同倍性杂交后代桑叶品质性状的遗传特性[J]. 中国农学通报，2018，34（15）：85-88
[13] REIS G B，MESQUITA A T，ANDRADE-VIEIRA L F，et al. Somatic cell alterations in interspecific hybrids of Cenchrus purpureum （Schumach.） and Cenchrus americanus （L.） Morrone by genomic in Situ hybridization[J]. Cytology and Genetics，2021，55（2）：194-198
[14] TECHIO V H，DAVIDE L C，PEREIRA A V，et al. Cytotaxonomy of some species and of interspecific hybrids of Pennisetum （Poaceae， Poales）[J]. Genetics and Molecular Biology，2002，25：203-209
[15] DIZ D A. Breeding procedures and seed production management in pearl millet×elephantgrass hexaploid hybrids[D]. Gainesville，Florida：University of Florida，1994：1-4
[16] ANDRADE-VIEIRA L F，REIS G B，TORRES G A，et al. Biparental chromosome elimination in artificial interspecific hybrids of Pennisetum purpureum and Pennisetum glaucum[J]. Crop Science，2013，53（5）：1917-1924
[17] YAN Q，WU F，XU P，et al. The elephant grass （Cenchrus purpureus） genome provides insights into anthocyanidin accumulation and fast growth[J]. Molecular Ecology Resources，2021，21（2）：526-542
[18] KRUGER M J，DAVIES N，MYBURGH K H，et al. Proanthocyanidins， anthocyanins and cardiovascular diseases[J]. Food Research International，2014，59：41-52
[19] YI X F，ZHOU H，SHI J，et al. Analysis of the effect of feeding goats with purple elephant grass[J]. Feed Research，2017（6）：48-52易显凤，周恒，史静，等. 紫色象草饲喂山羊效果分析[J]. 饲料研究，2017（6）：48-52
[20] ZHANG M Y，LI X H，WANG X，et al. Modulation of lignin and anthocyanin homeostasis by GTP cyclohydrolase1 in maize[J]. Plant Biotechnology Journal，2025，23（7）：2449-2463
[21] DANG J Q，WANG X，SU N N，et al. Induction and identification analysis of tetraploid Pennisetum alopecuroides ‘Ziguang’[J]. Acta Agrestia Sinica，2025，33（10）：3430-3437党佳旗，王鑫，苏妮妮，等. 四倍体‘紫光’狼尾草的诱导及鉴定分析[J]. 草地学报，2025，33（10）：3430-3437
[22] KANDEL R，SINGH H P，SINGH B P，et al. Assessment of genetic diversity in napier grass （Pennisetum purpureum Schum.） using microsatellite， single-nucleotide polymorphism and insertion-deletion markers from pearl millet （Pennisetum glaucum [L.] R. Br.）[J]. Plant Molecular Biology Reporter，2016，34：265–272
[23] YANG X Y，HU C X，LI S，et al. Investigation and analysis of anthocyanin content of 39 mulberry varieties[J]. Chinese Agricultural Science Bulletin，2025，41（13）：158-164杨星莹，胡昌雄，李莎，等. 39份桑葚品种花青素含量分析[J]. 中国农学通报，2025，41（13）：158-164
[24] OBOK E E. Post-zygotic reproductive barrier in pearl millet （Pennisetum glaucum （L.） R. Br.）×elephant grass （Pennisetum purpureum Schum.） interspecific F₁ hybrids[J]. Canadian Journal of Plant Breeding，2013，1（1）：1-5
[25] LEVIN D A. The origin of isolating mechanisms in flowering plants[J]. Evolutionary Biology，1978，11：185-315
[26] BOMBLIES K，WEIGEL D. Hybrid necrosis： autoimmunity as a potential gene flow barrier in plant species[J]. Nature Reviews Genetics，2007，8：382-393
[27] KAUSHAL P，KHARE A，SIDDIQUI S A，et al. Morphological， cytological and reproductive characterization of tri-species hybrids （GOS） between Pennisetum glaucum， P. orientale and P. squamulatum[J]. Euphytica，2010，174：261-281
[28] FANG X F，YUE Y S，TENG K，et al. A method for interspecific distant hybridization of panicum：China，CN120226605A[P]，2025-07-01范希峰，岳跃森，滕珂，等. 一种适用于狼尾草种间远缘杂交的方法：中国，CN120226605A[P]，2025-07-01
[29] JADE S S，TAKAWALE P S，BAHULIKAR R A. The utility of ISSRs for the identification of interspecific hybrids between pearl millet （Pennisetum glaucum [L.] R. Br.）×napier grass （Pennisetum purpureum Schumach）[J]. Plant Genetic Resources，2021，19（2）：104-111
[30] VARSHNEY R K，SHI C，THUDI M，et al. Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments[J]. Nature Biotechnology，2017，35（10）：969-976
[31] BARBOSA S，DAVIDE L C，PEREIRA A V. Cytogenetics of Pennisetum purpureum Schumack×Pennisetum glaucum L. hybrids and their parents[J]. Ciência e Agrotecnologia，2003，27（1）：26-35
[32] BABU C，SUNDARAMOORTHI J，VIJAYAKUMAR G，et al. Analysis of genetic diversity in napier grass （Pennisetum purpureum Schum） as detected by RAPD and ISSR markers[J]. Journal of Plant Biochemistry and Biotechnology，2009，18：181-187
[33] JOGAIAH S，SHARATHCHANDRA R G，RAJ N，et al. Development of SCAR marker associated with downy mildew disease resistance in pearl millet （Pennisetum glaucum L.）[J]. Molecular Biology Reports，2014，41：7815-7824
[34] PAUDEL D，KANNAN B，YANG X P，et al. Surveying the genome and constructing a high-density genetic map of napiergrass （Cenchrus purpureus Schumach）[J]. Scientific Reports，2018，8（1）：14419
[35] KANNAN B，VALENCIA E，ALTPETER F. Interspecific hybridization between elephantgrass and pearl millet and selection of hybrids with high-biomass production and enhanced biosafety[C].In： American Society of America （ASA）， Crop Science Society of America （CSSA） and Soil Science Society of America （SSSA） International Annual Meetings： Water， Food， Energy & Innovation for a Sustainable World Tampa.Florida，2013：165
[36] NI M，MA C Y，DONG H J，et al. Analysis of nutritional evaluation and swainsonine of Oxytropis glabra DC[J]. Modern Animal Husbandry Science & Technology，2024（9）：75-78倪苗，马崇勇，董海娟，等. 小花棘豆苦马豆素和营养成分分析[J].现代畜牧科技，2024（9）：75-78
[37] MUSTAFA A F，SÉGUIN P，BELAIR G，et al. Chemical composition and ruminal degradability of grain pearl millet grown in southwestern Quebec[J]. Canadian Journal of Animal Science，2008，88（1）：71-77
[38] LIANG J Q，LIU R X，GUO Z B. Feeding value of quinoa and its effect on growth performance and economic benefits of growing cattle[J]. China Feed，2024（5）：143-148梁嘉琪，刘瑞香，郭占斌. 藜麦的饲用价值及对育成牛生长性能与经济效益的影响[J]. 中国饲料，2024（5）：143-148
[39] WANG X C，HAN P，WANG W，et al. Study on mixed silage of alfalfa and forage oats[J]. Modern Animal Husbandry Science & Technology，2024（4）：74-76王晓春，韩鹏，汪伟，等. 紫花苜蓿与饲用燕麦混合青贮研究[J]. 现代畜牧科技，2024（4）：74-76
[40] ZHANG L，CHEN S F，HUANG G F，et al. Research development and prospect of mixed silage[J]. Feed Research，2021，44（6）：151-153张磊，陈思凡，黄国锋，等. 混合青贮研究进展及前景[J]. 饲料研究，2021，44（6）：151-153
[41] ZHANG X P，WAN L Q，LI X L，et al. The effect of adding lactic acid bacteria and cellulase on Medicago sativa silage quality[J]. Acta Prat Aculturae Sinica，2007，16（3）：139-143张新平，万里强，李向林，等. 添加乳酸菌和纤维素酶对苜蓿青贮品质的影响（简报）[J]. 草业学报，2007，16（3）：139-143
[42] LI M W，YANG C J，PENG K P，et al. Study on the quality of Pennisetum purpureum Schum silage with different additives[J]. Acta Ecologiae Animalis Domastici，2019，40（6）：33-37李孟伟，杨承剑，彭开屏，等. 添加不同物料对象草青贮品质影响的研究[J]. 家畜生态学报，2019，40（6）：33-37

‘宁杂4号’美洲狼尾草×‘紫色’象草三倍体杂交种的创制与高产高花青素优系筛选

Creation of a Triploid Hybrid of Pennisetum glaucum ‘Ningza No.4’×P. purpureum ‘purple’ and Screening for High-yielding， High-antocyanin Superior Lines

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