草地学报

›› 2010, Vol. 18 ›› Issue (2): 297-301.DOI: 10.11733/j.issn.1007-0435.2010.02.029

• 研究论文 • 上一篇    下一篇

紫花苜蓿花粉生活力测定方法研究

陈晶1, 张月学2, 唐凤兰2, 刘凤歧2, 韩微波2, 刘杰淋2, 陈积山2, 尚晨2, 张海玲2   

  1. 1. 哈尔滨师范大学生命科学与技术学院生物工程与技术系, 黑龙江, 哈尔滨, 150025;
    2. 黑龙江省农业科学院草业研究所, 黑龙江, 哈尔滨, 150086
  • 收稿日期:2009-09-28 修回日期:2009-11-06 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 张月学,E-mail:zyxnky@163.com
  • 作者简介:陈晶(1984- ),女,黑龙江哈尔滨人,硕士研究生,研究方向为牧草遗传育种
  • 基金资助:
    牧草现代产业技术体系研究与建立项目(nyhyzx07-022);小宗作物空间环境诱变育种关键技术研究与示范项目(2008BAD97B07);黑龙江省农科院创新工程资助项目(200705)

Methodology Study on Measurement of Alfalfa Pollen Vitality

CHEN Jing1, ZHANG Yue-xue2, TANG Feng-lan2, LIU Feng-qi2, HAN Wei-bo2, LIU Jie-lin2, CHEN Ji-shan2, SHANG Chen2, ZHANG Hai-ling2   

  1. 1. Department of Biological Engineering and Technology, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang Province 150080, China;
    2. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China
  • Received:2009-09-28 Revised:2009-11-06 Online:2010-04-15 Published:2010-04-15

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摘要： 为找到快速而有效地测定紫花苜蓿(Medicago sativa L.)花粉生活力的方法,本文通过碘-碘化钾染色、氯化三苯基四氮唑(TTC)染色、红墨水染色以及花粉离体培养萌发4种方法测定紫花苜蓿花粉的生活力。结果表明:前3种染色的方法不能准确快速的测定肇东苜蓿花粉生活力,离体培养萌发法中的琼脂法和悬滴法可以快速而准确的测定肇东苜蓿花粉的生活力。琼脂法和悬滴法培养花粉1.5h其萌发率最高,平均萌发率为55%,但悬滴法更加快捷方便,萌发率是54%,最适宜的浓度为蔗糖10%,硼酸0.015%。

关键词: 紫花苜蓿花粉, 生活力, 离体培养, 萌发率

Abstract: The aim of this paper was to find out a quick and effective method for pollen vitality measurement of alfalfa.The candidate methods tested in this paper were I2-KI dyeing,TTC dyeing,red ink dyeing,and in vitro germination.The results show that the former three dyeing methods couldn't accomplish the target.Among in vitro germination methods,agar and hanging drop realized the quick and effective measurement of alfalfa pollen vitality;especially the germination rate of the pollen cultivated for 1.5 h reached to an average of 55%.However,the hanging drop method was quicker and more convenient and its germination rate could reach to 54%.The optimal nutrient solution contained 10% sucrose and 0.015% boric acid.

Key words: Alfalfa pollen, Vitality, Isolated culture, Germination rate

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