NaCl和等渗PEG4000胁迫对紫花苜蓿种子发芽及生理活性的影响

doi:10.11733/j.issn.1007-0435.2012.03.026

草地学报 ›› 2012, Vol. 20 ›› Issue (3): 547-552.DOI: 10.11733/j.issn.1007-0435.2012.03.026

NaCl和等渗PEG4000胁迫对紫花苜蓿种子发芽及生理活性的影响

马巧利¹, 孙彦¹, 杨青川², 熊军波³

1. 中国农业大学动物科技学院草业科学系, 北京 100193;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 湖北省农业科学院畜牧兽医研究所, 湖北武汉 430064

收稿日期:2011-10-19 修回日期:2011-12-03 出版日期:2012-06-15 发布日期:2012-07-05
通讯作者: 孙彦,E-mail:ctsoffice@yahoo.com.cn
作者简介:马巧利(1987- ),女,宁夏固原人,硕士研究生,研究方向为草坪分子生物学,E-mail:mql_2008@126.com
基金资助:
国家牧草产业技术体系岗位科学家课题(CARS-35)资助

Influence of NaCl and Osmotic PEG4000 Stress on Seed Germination of Medicago sativa

MA Qiao-li¹, SUN Yan¹, YANG Qing-chuan², XIONG Jun-bo³

1. Institute of Grassland Science, China Agricultural University, Beijing 100193, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Institute of Animal Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei Province 430064, China

Received:2011-10-19 Revised:2011-12-03 Online:2012-06-15 Published:2012-07-05

摘要/Abstract

摘要： 为比较等渗的NaCl和PEG溶液模拟盐分和水分胁迫条件对紫花苜蓿(Medicago sativa)种子发芽过程的影响,选取5个紫花苜蓿品种分别对其种子发芽率和胚根长度进行统计。结果表明:5个品种受2种胁迫后,其发芽率和胚根长度变化趋势相似,即NaCl能显著降低紫花苜蓿种子的发芽率(P<0.05),PEG4000则影响不大;其胚根长度变化趋势为:受NaCl胁迫后胚根较粗短,PEG4000胁迫后细而长。对受2种胁迫影响较小的中苜三号发芽后其电导率、脯氨酸和叶绿素含量的测定发现,胁迫后电导率均增大,但受NaCl胁迫处理的差异极显著(P<0.01);游离脯氨酸积累均增高,其中PEG4000胁迫处理后,脯氨酸的积累极显著增高(P<0.01);中苜3号受等渗NaCl胁迫后,叶绿素含量最低,对照是其含量的2.3倍(P<0.01),受PEG4000处理后叶绿素含量有所上升,但与对照相比差异不显著。由此说明等渗胁迫条件下,盐胁迫比干旱胁迫对中苜三号的伤害更大。

关键词: 紫花苜蓿, NaCl, PEG, 等渗胁迫, 种子发芽

Abstract: In order to understand the influence of both NaCl and osmotic PEG4000 stress on seed germination of Medicago sativa, five varieties of alfalfa seeds were selected to determine their germination rates and radicle lengths. Results show that germination rate and radicle length of alfalfa seeds have similar trends between five varieties under both NaCl and osmotic PEG4000 stress. NaCl can significantly reduce alfalfa seed germination rate, while PEG4000 has little influence (P<0.05). Germinated seeds show short, thick radicle length with NaCl stress, while they appear as thin, long radicle lengths with PEG4000 stress. Medicago sativa 'Zhongmu No.3’ shows less influence with NaCl stress than osmotic PEG4000 stress. Relative conductivities are reduced with NaCl stress while proline content is significantly increased with PEG4000 stress (P<0.01). Chlorophyll contents show the lowest levels with NaCl stress, but show a slight rise with PEG4000 stress. In summary, salt stress appears more harmful than drought stress for Medicago sativa 'Zhongmu No.3’.

Key words: Medicago sativa, NaCl, PEG, Ostomic stress, Seed germination

中图分类号:

Q945.78
S541

马巧利, 孙彦, 杨青川, 熊军波. NaCl和等渗PEG4000胁迫对紫花苜蓿种子发芽及生理活性的影响[J]. 草地学报, 2012, 20(3): 547-552.

MA Qiao-li, SUN Yan, YANG Qing-chuan, XIONG Jun-bo. Influence of NaCl and Osmotic PEG4000 Stress on Seed Germination of Medicago sativa[J]. Acta Agrestia Sinica, 2012, 20(3): 547-552.

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参考文献

[1] FAO. Global network on integrated soil management for sustainable use of salt-affected soils . Rome, Italy: FAO Landand Plant Nutrition Management Service, 2005
[2] Munns R. Comparative physiology of salt and water stress [J]. Plant, Cell and Environment,2002,25(2):239-250
[3] Myrene R, D’Souza, Varadahally Rangaiah Devaraj. Biochemical responses of Hyacinth bean (Lablab purpureus) to salinity stress[J]. Acta Physiologiae Plantarum,2010,32(2):341-353
[4] Xiao yan XU, Rui FAN, Rui ZHENG, et al. Proteomic analysis of seed germination under salt stress in soybeans[J]. Journal of Zhejiang University-Science B: Biomedicine & Biotechnology,2011,12(7):507-517
[5] 李源,刘贵波,高洪文,等.NaCl胁迫对不同苜蓿种质苗期生长特性的影响[J].华北农学报,2010,25(S1):109-110
[6] Ildiko Kerepesi, Gabor Galiba. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings[J]. Crop Science,2000,40(2):482-487
[7] 中国科学院上海植物生理研究所.现代植物生理实验指南[M].北京:科学出版社,1999:302-306
[8] Munns R, Tester M. Mechanisms of salinity tolerance[J]. The Annual Review of Plant Biology,2008,59:651-681
[9] 王玉祥,陈爱萍,张博.不同苜蓿品种耐盐性初探[J].草业科学,2010,27(3):102-106
[10] 景艳霞,袁庆华.不同钠盐胁迫对苜蓿种子萌发的影响[J].种子,2010,29(2):69-72
[11] 田雨,武祎,郑伟.黄花苜蓿种质发芽及早期生长对NaCl胁迫的响应[J].中国草地学报,2009,31(6):25-29
[12] Wioletta Brosowska-Arendt,Stanisaw Weidner. Effect of osmotic stress on the formation of a populationof polysomes and their stability in pea (Pisum sativum L.) seeds[J]. Acta Physiologiae Plantarum,2011,33(4):1475-1482
[13] 於丽华,耿贵.等渗胁迫下NaCl和PEG对甜菜种子发芽和幼苗生长的影响[J].中国糖料,2008(3):33-39
[14] Miyake Y, E Takahashi. Effect of silicon on the growth of cucumber plant in soil culture[J].Soil Science and Plant Nutrition,1983,29(4):463-471
[15] 王赫,刘利,周道玮. 4种苜蓿属植物幼苗的抗旱性研究[J].草地学报,2010,18(2):205-210
[16] 李波,贾秀峰,白庆武,等.干旱胁迫对苜蓿脯氦酸累积的影响[J].植物研究2003,23(2):189-191
[17] 云岚,米福贵,云锦凤,等.六个苜蓿品种幼苗对水分胁迫的响应及其抗早性[J].中国草地,2004,26(2):25-28
[18] Davies W J, Zhang J. Root signal and the regulation of growth and development of plants in drying soil[J].Annual Review of Plant Physiology and Plant Molecular Biology,1991,42:55-76
[19] 杨锐.几种宁夏栽培紫花苜蓿萌发期及幼苗期在PEG-6000、NaCl复合胁迫下的响应银川:宁夏大学,2008:13-15
[20] P B Kishor, S Sangam, R N Amrutha,et al.Regulation of proline biosynthesis,degradation,uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance[J].Current Science,2005,88(3):424-438
[21] 吕世杰.黄花苜蓿抗旱、耐盐生理特性及其抗性机理的初步研究.呼和浩特:内蒙古农业大学,2007:14-15
[22] 王瑞瑞,宿婷,谢应忠.苜蓿6个品种幼苗期抗旱性比较研究[J].农业科学研究,2010,31(1):9-12

NaCl和等渗PEG4000胁迫对紫花苜蓿种子发芽及生理活性的影响

Influence of NaCl and Osmotic PEG4000 Stress on Seed Germination of Medicago sativa

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