›› 2013, Vol. 21 ›› Issue (6): 1148-1156.DOI: 10.11733/j.issn.1007-0435.2013.06.018

• 研究论文 • 上一篇    下一篇

荻不同资源种子萌发期抗盐性评价

宗俊勤, 高艳芝, 陈静波, 聂东阳, 刘建秀   

  1. 江苏省中国科学院植物研究所(南京中山植物园), 江苏 南京 210014
  • 收稿日期:2013-10-02 修回日期:2013-11-19 出版日期:2013-12-15 发布日期:2013-11-27
  • 通讯作者: 刘建秀
  • 作者简介:宗俊勤(1980-),男,江苏盐城人,助理研究员,硕士,主要从事禾草资源评价与育种工作,E-mail:zongjq1980@163.com
  • 基金资助:
    国家自然科学基金(31201262);江苏省自然科学基金(BK2012790)资助

Assessment of Salinity Tolerance of Miscanthus scchariflora Germplasm during Germination Period

ZONG Jun-qin, GAO Yan-zhi, CHEN Jing-bo, NIE Dong-yang, LIU Jian-xiu   

  1. Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China
  • Received:2013-10-02 Revised:2013-11-19 Online:2013-12-15 Published:2013-11-27

摘要: 用不同浓度NaCl溶液处理26份荻(Miscanthus scchariflora)资源种子,研究盐胁迫对其相对发芽率、发芽势、相对胚根长度的影响,并通过聚类分析法对其抗盐性进行综合评价,以期为荻资源的抗盐性研究提供理论依据。结果表明:随着NaCl浓度的增加,除01144外其余材料的相对发芽率和发芽势均呈下降趋势,所有材料的胚根生长均受到越来越明显的抑制作用。不同指标的变异系数随着NaCl浓度的增加呈现明显升高的趋势,表明盐浓度越高,不同材料对盐胁迫的适应性差异越大。通过聚类分析可将材料分为3类,即极抗盐型,只包括01144;抗盐型,包括11019,12141,12183,01202,11017,12101,12126,12178,01038,01094,01118和01143;敏盐型,包括12006,12023,12051,12055,12078,12135,12154,01073,01305,12184,12189,01149和01252。

关键词: 荻, 种质资源, 盐胁迫, 种子萌发, 变异, 聚类

Abstract: The relative germination rate, germination potentiality and relative radicle length of Miscanthus scchariflora seed were investigated after treated with different NaCl concentrations. The salinity tolerances of tested materials were evaluated by cluster analysis to provide a theoretical foundation for the salinity tolerance research of M. scchariflora germplasm. It was found that the relative germination rates and germination potentialities of tested accessions except for 01144 decreased with the increase of NaCl concentration, and the relative radicle lengths of all tested accessions decreased. In addition, the coefficient of variation of all tested parameters obviously increased with the increase of NaCl concentration indicating that the adaptations of different M. scchariflora germplasms to salt stress had greater difference under higher NaCl concentration. Cluster analysis indicated that twenty-six accessions were divided into three categories according to salinity tolerance. Accession No. 01144 had the highest salinity tolerance among tested materials. The salt-tolerant group had the accessions of 11019, 12141, 12183, 01202, 11017, 12101, 12178, 01038, 01094, 01118 and 01143. The salt-sensitive group included the accessions of 12006, 12023, 12051, 12055, 12078, 12135, 12154, 01073, 01305, 12184, 12189, 01149 and 01252.

Key words: Miscanthus scchariflora, Germplasm, Salt stress, Seed germination, Variation, Cluster

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