牛心朴子内生真菌分离及其抑菌活性分析

doi:10.11733/j.issn.1007-0435.2014.01.029

›› 2014, Vol. 22 ›› Issue (1): 188-193.DOI: 10.11733/j.issn.1007-0435.2014.01.029

牛心朴子内生真菌分离及其抑菌活性分析

毕江涛¹, 潘润霞², 李乐², 何萍², 黄盼盼², 王静¹

1. 宁夏大学新技术应用研发开发中心, 宁夏银川 750021;
2. 宁夏大学生命科学学院, 宁夏银川 750021

收稿日期:2013-06-04 修回日期:2013-08-24 出版日期:2014-02-15 发布日期:2014-01-28
作者简介:毕江涛（1964-），男，宁夏中宁人，博士，副研究员，主要从事微生物生态与微生物资源研究，E-mail：731507283@qq.com
基金资助:
宁夏银川市科技攻关项目（银财2010452）资助

Isolation and Microbial Inhibition Activity of Endophytic Fungus from Cynanchum komarovii

BI Jiang-tao¹, PAN Run-xia², LI Le², HE Ping², HUANG Pan-pan², WANG Jing¹

1. Research and Development Center for New Techniques, Ningxia University, Yichuan, Ningxia 750021, China;
2. College of Life Sciences, Ningxia University, Yinchuan, Ningxia 750021, China

Received:2013-06-04 Revised:2013-08-24 Online:2014-02-15 Published:2014-01-28

摘要/Abstract

摘要： 为了解荒漠植物牛心朴子（Cynanchum komarovii）内生真菌多样性及其抑菌活性特征，通过组织块法分离其内生真菌，并选择5种植物病原真菌和4种人体病原细菌作为指示菌测定抑菌活性。结果表明：分离的25株内生真菌以茎部最多，根部次之，叶部最少，经形态学初步鉴定归于2目3科11属，曲霉属（Aspergillus）为优势菌属；84%的分离菌株对2种以上病原真菌，以及60%的分离菌株对1种以上病原细菌有不同程度的拮抗作用，2株高活性菌株CKEFL003和CKEFS001对多种病原菌拮抗作用明显，均为曲霉属。牛心朴子内生真菌在根茎叶组织器官中数量分布存在差异，种群构成具有多样性特征，对植物和人体病原菌具有不同程度的拮抗作用，抑菌资源较为丰富，高活性菌株为曲霉属，抗菌谱较宽。

关键词: 荒漠植物, 牛心朴子, 内生真菌, 分离, 抑菌活性

Abstract: To understand the diversity and microbial inhibition activities of endophytic fungi from desert plant, endophytic fungi were isolated from the root, stem and leaf of Cynanchum komarovii by tissue inoculation culture. The microbial inhibition activities of the endophytic fungi were tested using five pathogenic fungi strains and four pathogenic bacteria strains as indicators. Results indicated that twenty-five fungal endophytic strains were isolated and most of them were from stem. The isolated strains belonged to eleven genera, three families and two orders based on morphological characteristics. The 84.0% strains of total isolates had inhibitory activities against two or more tested fungal pathogens, and 60.0% strains had inhibitory activities against one or more tested pathogenic bacteria. Two strains were identified as Aspergillus, having obvious inhibitory activities against tested pathogens. The distribution of endophytes had significant difference among the root, stem and leaf of Cynanchum komarovii. The population of the isolates had the characteristics of diversity with different inhibition activities showing the abundant resources of pathogenic inhibition. The two strong active strains of Aspergillus had relatively broad inhibition spectrum.

Key words: Desert plant, Cynanchum komarovii, Endophytic fungi, Isolation, Inhibitory activity

中图分类号:

Q946

毕江涛, 潘润霞, 李乐, 何萍, 黄盼盼, 王静. 牛心朴子内生真菌分离及其抑菌活性分析[J]. , 2014, 22(1): 188-193.

BI Jiang-tao, PAN Run-xia, LI Le, HE Ping, HUANG Pan-pan, WANG Jing. Isolation and Microbial Inhibition Activity of Endophytic Fungus from Cynanchum komarovii[J]. , 2014, 22(1): 188-193.

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牛心朴子内生真菌分离及其抑菌活性分析

Isolation and Microbial Inhibition Activity of Endophytic Fungus from Cynanchum komarovii

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