内生真菌对醉马草不同生长期土壤酶活性和养分的影响

doi:10.11733/j.issn.1007-0435.2024.06.013

草地学报 ›› 2024, Vol. 32 ›› Issue (6): 1770-1778.DOI: 10.11733/j.issn.1007-0435.2024.06.013

• 研究论文 • 上一篇

内生真菌对醉马草不同生长期土壤酶活性和养分的影响

刘荣贵¹, 邓茂桦¹, 张瀚文¹, 郑蓉¹, 唐仲龙², 王超¹, 王剑峰¹

1. 兰州大学草种创新和草地农业生态系统全国重点实验室/兰州大学草地微生物研究中心/兰州大学草地农业科技学院, 甘肃兰州 730020;
2. 临夏州农业科学院, 甘肃临夏 731100

收稿日期:2023-10-26 修回日期:2023-12-06 发布日期:2024-06-29
通讯作者: 王剑峰,E-mail:wangjf12@lzu.edu.cn;唐仲龙,E-mail:tzldyx2023@163.com
作者简介:刘荣贵(2001-)，男，汉族，湖南衡阳人，硕士研究生，主要从事禾草内生真菌抗逆生物学研究，E-mail：liurg2023@lzu.edu.cn
基金资助:
国家自然科学基金(32001399，32371772)；兰州大学中央高校基础研究基金(lzujbky-2021-ey01)资助

Effects of Epichloë gansuensis Endophyte on Soil Enzyme Activities and Nutrients at Different Growth Periods of Achnatherum inebrians

LIU Rong-gui¹, DENG Mao-hua¹, ZHANG Han-wen¹, ZHENG Rong¹, TANG Zhong-long², WANG Chao¹, WANG Jian-feng¹

1. State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, Lanzhou University;Center for Grassland Microbiome, Lanzhou University;College of Pastoral Agriculture Science and Technology;Lanzhou University, Lanzhou, Gansu Province 730020, China;
2. Linxia Academy of Agricultural Sciences, Linxia, Gansu Province 731100, China

Received:2023-10-26 Revised:2023-12-06 Published:2024-06-29

摘要/Abstract

摘要： 醉马草-内生真菌共生体是研究生态系统中地上与地下生物联系的独特模式，但该共生体对不同生长期土壤酶活性和养分含量的影响知之甚少。本研究采集休眠期和返青期携带内生真菌(E+)和未携带内生真菌(E-)醉马草的生境土壤，测定E+和E-醉马草土壤酶活性和养分含量。结果表明：在休眠期和返青期，内生真菌显著增加了醉马草土壤速效磷含量，较E-醉马草分别提高34.7%和36.7%。在返青期内生真菌增加了醉马草土壤硝态氮含量，较E-醉马草提高了32.3%。此外，返青期温度升高增强了E+和E-醉马草土壤蔗糖酶和碱性磷酸酶活性，这提高了土壤N、P的有效性，为醉马草的生长返青提供基本养分。以上结果表明内生真菌通过调节土壤养分提高土壤肥力，返青期土壤酶活性和养分含量的变化保证了醉马草顺利返青。本研究为深入理解禾草-内生真菌共生体的生态学作用提供理论依据。

关键词: 醉马草, 内生真菌, 土壤酶活性和养分含量, 休眠期和返青期

Abstract: The Achnatherum inebrians-Epichloë endophyte symbiosis is a unique model for studying the aboveground and belowground biological linkages in ecosystems,but little is known about the effects of this symbiosis on soil enzyme activities and nutrient content at different growth periods. In this study,we collected soils from drunken horse grass infected with endophytic fungi (E+) and without endophytic fungi (E-) during periods of dormancy and regeneration,and determined the enzyme activities and nutrient contents of E+ and E-drunken horse grass habitat soils. The results showed that endophytic fungi significantly increased the available phosphorus content of drunken horse grass soils by 34.7% and 36.7% over E-drunken horse grass during the periods of dormancy and regeneration,respectively. In addition,endophytic fungi increased the nitrate nitrogen content of drunken horse grass soil during the regeneration period by 32.3% compared to E-soil. During the regeneration period,the increased temperature enhanced the activities of sucrase and alkaline phosphatase in E+ and E-drunken horse grass soils,which in turn increased the effectiveness of soil N and P,and provided essential nutrients for the growth and regeneration of drunken horse grass. The above results indicated that the endophytic fungi improved soil fertility by regulating soil nutrients,and the changes in soil enzyme activities and nutrient contents during the regeneration period ensured the successful regeneration of drunken horse grass. This study provides a theoretical basis for a deeper understanding of the ecological role of grass-endophytic fungi symbiosis.

Key words: Achnatherum inebrians, Epichloë gansuensis, Soil enzyme activities and nutrients content, Dormancy and regeneration period

中图分类号:

S154.1

刘荣贵, 邓茂桦, 张瀚文, 郑蓉, 唐仲龙, 王超, 王剑峰. 内生真菌对醉马草不同生长期土壤酶活性和养分的影响[J]. 草地学报, 2024, 32(6): 1770-1778.

LIU Rong-gui, DENG Mao-hua, ZHANG Han-wen, ZHENG Rong, TANG Zhong-long, WANG Chao, WANG Jian-feng. Effects of Epichloë gansuensis Endophyte on Soil Enzyme Activities and Nutrients at Different Growth Periods of Achnatherum inebrians[J]. Acta Agrestia Sinica, 2024, 32(6): 1770-1778.

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参考文献

[1] 李春杰,姚祥,南志标. 醉马草内生真菌共生体研究进展[J]. 植物生态学报,2018,42(8):793-805
[2] 姚祥. 醉马草内生真菌共生体对草地植物组成及土壤微生物群落多样性的影响[D]. 兰州:兰州大学,2019:1-10
[3] 何雅丽. 外源激素对内生真菌-醉马草共生体的诱导抗虫性研究[D]. 兰州:兰州大学,2021:53
[4] 夏超. 醉马草-内生真菌共生体对干旱胁迫的响应[D]. 兰州:兰州大学,2018:92-95
[5] WANG C,HUANG R,WANG J F,et al. Comprehensive analysis of transcriptome and metabolome elucidates the molecular regulatory mechanism of salt resistance in roots of Achnatherum inebrians mediated by Epichloë gansuensis[J]. Journal of Fungi,2022,8(10):1092-1092
[6] JIN J,WANG C,LIU R G,et al. Soil microbial community compositions and metabolite profiles of Achnatherum inebrians affect phytoremediation potential in Cd contaminated soil[J]. Journal of Hazardous Materials,2023,459:132280-132280
[7] 刘英龙. 醉马草-内生真菌共生体响应低磷的分子机制研究[D]. 兰州:兰州大学,2023:89-90
[8] 连鹤娜. 坪用驱鸟型醉马草种质材料筛选与成坪技术研究[D]. 兰州:兰州大学,2023:1
[9] MOSADDEGHI M R,HOSSEINI F,HAJABBASI M A,et al. Chapter Two-Epichloë spp. and Serendipita indica endophytic fungi:Functions in plant-soil relations[J]. Advances in Agronomy,2021,165:59-113
[10] 李敏,吴长友. 几种土壤生物因子对土壤养分的影响[J]. 土壤通报,2007(1):43-46
[11] ADETUNJI A T,LEWU F B,et al. The biological activities of β-glucosidase,phosphatase and urease as soil quality indicators:a review[J]. Journal of Soil Science and Plant Nutrition,2017,17(3):794-807
[12] 金媛媛,陈振江,王添,等. 内生真菌和田间管理措施对土壤真菌群落丰度和多样性的影响[J]. 草业学报,2023,32(4):142-152
[13] ROJAS X,GUO J,LEFF J W,et al. Infection with a shoot-specific fungal endophyte (Epichloë) alters tall fescue soil microbial communities[J]. Microbial Ecology,2016,72(1):197-206
[14] 金媛媛,SAMAN B,贾倩民,等. 内生真菌侵染对野大麦根际土壤化学特性和微生物群落的影响[J]. 草业学报,2019,28(10):66-77
[15] ZHANG Y,CUI D,YANG H,et al. Differences of soil enzyme activities and its influencing factors under different flooding conditions in Ili Valley,Xinjiang[J]. PeerJ,2020,8:e8531
[16] 谢乐乐,王晓丽,马源,等. 返青期休牧对高寒草甸植物品质与碳氮磷化学计量比的影响[J]. 草地学报,2023,31(5):1454-1460
[17] HOU W P,XIA C,CHRISTENSEN M J,et al. Effect of Epichloë gansuensis endophyte on rhizosphere bacterial communities and nutrient concentrations and ratios in the perennial grass species Achnatherum inebrians during three growth seasons[J]. Crop and Pasture Science,2020,71(11-12):1050-1066
[18] 曹晏风. 兰州市大气降水水化学时空变化及影响因子研究[D]. 兰州:西北师范大学,2021:13
[19] ZHONG R,XIA C,JU Y,et al. A foliar Epichloë endophyte and soil moisture modified belowground arbuscular mycorrhizal fungal biodiversity associated with Achnatherum inebrians[J]. Plant and Soil,2019,458(1-2):1-18
[20] AKHTAR K,WANG W,REN G,et al. Changes in soil enzymes,soil properties,and maize crop productivity under wheat straw mulching in Guanzhong,China[J]. Soil and Tillage Research,2018,182:94-102
[21] ZHANG X,FAN X,LI C,et al. Effects of cadmium stress on seed germination,seedling growth and antioxidative enzymes in Achnatherum inebrians plants infected with a Neotyphodium endophyte[J]. Plant Growth Regulation,2010,60(2):91-97
[22] TIAN H,KONG L,MEGHARAJ M,et al. Contribution of attendant anions on cadmium toxicity to soil enzymes[J]. Chemosphere,2017,187:19-26
[23] NELSON D W,SOMMERS L E. Total carbon,organic carbon,and organic matter in:methods of soil analysis part 2. chemical and microbial properties[J]. Plant Physiology,1996:961-1010
[24] ZHANG L M,DUFF A M,SMITH A J. Community and functional shifts in ammonia oxidizers across terrestrial and marine (soil/sediment) boundaries in two coastal Bay ecosystems[J]. Environmental microbiology,2018,20(8):2834-2853
[25] 王明涛,雷变霞,赵玉红,等. 垂穗披碱草人工草地建植和管理措施对土壤真菌群落的影响[J]. 草地学报,2023,31(6):1728-1734
[26] FAN H B,WU J P,LIU W F,et al. Linkages of plant and soil C∶N∶P stoichiometry and their relationships to forest growth in subtropical plantations[J]. Plant and Soil,2015,392(1/2):127-138
[27] 林丽,张德罡,曹广民,等. 放牧强度对高寒嵩草草甸土壤养分特性的影响[J]. 生态学报,2016,36(15):4664-4671
[28] 孙一丹,张兴旭,古丽君,等. 醉马草-内生真菌共生体中生物碱的抑菌活性[J]. 草业科学,2015,32(4):8-514
[29] GUNDEL P E,HELANDER M,GARIBALDI L A,et al. Role of foliar fungal endophytes in litter decomposition among species and population origins[J]. Fungal Ecology,2016,21:50-56
[30] PURAHONG W,HYDE D. Effects of fungal endophytes on grass and non-grass litter decomposition rates[J]. Fungal Diversity,2011,47(1):1-7
[31] MüLLER K,MARHAN S,KANDELER E,et al. Carbon flow from litter through soil microorganisms:from incorporation rates to mean residence times in bacteria and fungi[J]. Soil Biology and Biochemistry,2017,115:187-196
[32] 金媛媛,BOWATTE S,田沛,等. 禾草-内生真菌共生对土壤理化性质及其微生物影响的研究进展[J]. 草业科学,2019,36(5):1292-1307
[33] 郭长辉,李秀璋,柳莉,等.内生真菌对醉马草根际土壤线虫群落的影响[J]. 草业学报,2016,25(4):140-148
[34] 杨振辉. 禾草-内生真菌共生体对宿主凋落物分解的影响及其机制[D]. 兰州:兰州大学,2022:25-26
[35] 詹寿发,卢丹妮,毛花英,等. 2株溶磷、解钾与产IAA的内生真菌菌株的筛选、鉴定及促生作用研究[J]. 中国土壤与肥料,2017(3):142-151
[36] CHEN Z J,WHITE J F,MALIK K,et al. Soil nutrient dynamics relate to Epichloë endophyte mutualism and nitrogen turnover in a low nitrogen environment[J]. Soil Biology and Biochemistry,2022,174:108832
[37] FENNER N,FREEMAN C,REYNOLDS B. Observations of a seasonally shifting thermal optimum in peatland carbon-cycling processes;implications for the global carbon cycle and soil enzyme methodologies[J]. Soil Biology and Biochemistry,2005,37(10):1814-1821
[38] KANG H J,KIM S Y,FENNER N,et al. Shifts of soil enzyme activities in wetlands exposed to elevated CO₂[J]. Science of the Total Environment,2005,337(1-3):207-212
[39] 吴秀臣,孙辉,杨万勤. 土壤酶活性对温度和CO₂浓度升高的响应研究[J]. 土壤,2007(3):358-363
[40] 常文华,马维伟,李广,等. 尕海湿地不同退化梯度土壤脲酶与蛋白酶活性时空分布特征[J]. 土壤,2022,54(3):524-531
[41] 李锐,陶瑞,王丹,等. 减氮配施有机肥对滴灌棉田土壤生物学性状与团聚体特性的影响[J]. 应用生态学报,2017,28(10):3297-3304
[42] HOU W P,WANG J F,NAN Z B,et al. Epichloë gansuensis endophyte-infection alters soil enzymes activity and soil nutrients at different growth stages of Achnatherum inebrians[J]. Plant and Soil,2020,455:227-240

内生真菌对醉马草不同生长期土壤酶活性和养分的影响

Effects of Epichloë gansuensis Endophyte on Soil Enzyme Activities and Nutrients at Different Growth Periods of Achnatherum inebrians

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