蒺藜苜蓿种子休眠机制及其破除方法研究

doi:10.11733/j.issn.1007-0435.2015.02.021

草地学报 ›› 2015, Vol. 23 ›› Issue (2): 358-365.DOI: 10.11733/j.issn.1007-0435.2015.02.021

蒺藜苜蓿种子休眠机制及其破除方法研究

刘文瑜¹, 魏小红^1,2, 许可成¹, 赵萌¹, 段正中³

1. 甘肃农业大学生命科学技术学院, 甘肃兰州 730070;
2. 甘肃省干旱生境作物学重点实验室甘肃农业大学, 甘肃兰州 730070;
3. 甘肃省武威市凉州区和平镇人民政府农业办, 甘肃武威 733000

收稿日期:2014-07-14 修回日期:2014-12-08 出版日期:2015-04-15 发布日期:2015-03-25
通讯作者: 魏小红
作者简介:刘文瑜(1985-),女,甘肃兰州人,博士研究生,研究方向为植物逆境生态生理,E-mail:yu850721.lemon@163.com
基金资助:
甘肃省自然基金项目 (2014GS04173)资助

Study on Dormancy Property and Breaking Approaches of Medicago truncatula Seed

LIU Wen-yu¹, WEI Xiao-hong^1,2, XU Ke-cheng¹, ZHAO Meng¹, DUAN Zheng-zhong³

1. Colledge of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Gansu Provincial Key Laboratory of Agridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
3. Agricultural office of People's Government in Heping Zhen, Liangzhou District, Wuwei, Gansu Province 733000, China

Received:2014-07-14 Revised:2014-12-08 Online:2015-04-15 Published:2015-03-25

摘要/Abstract

摘要：

以蒺藜苜蓿(Medicago truncatula)种子为试验材料,通过测定种子生活力、发芽率、吸水率及粗提物的生物活性;并用不同浓度的赤霉素(GA₃)浸种后4℃低温层积或同浓度硝普钠(SNP)浸种,揭示蒺藜苜蓿种子休眠原因及破除方法.结果表明:蒺藜苜蓿种子具有生活力;30 d内裸粒种子发芽率为100%;破皮种子的吸水率显著高于完整种子;随着蒺藜苜蓿各部分粗提物浓度的升高,白三叶(Trifolium repens)草种子萌发和幼苗生长显著降低;0.1~0.7 mmo·L^-1的GA₃浸种后低温层积或同浓度SNP浸种均可以显著提高种子的发芽率,其中以0.1 mmol·L^-1 GA₃浸种后低温层积或同浓度的SNP处理效果最好.表明蒺藜苜蓿种子的休眠属于综合性休眠,可用GA₃浸种后低温层积或SNP浸种来解除其休眠.

关键词: 蒺藜苜蓿, 种子, 休眠, 赤霉素, 萌发

Abstract:

Using Medicago truncatula seeds as experimental material, the dormancy mechanism of Medicago truncatula seeds was discussed through the determination of seed viability, germination rate, water absorption rate and biological activity tests of the crude extracts; and the breaking methods of seeds were explored with pre-soaking in different concentrations of GA₃ or SNP. Experimental results showed that the staining life force of Medicago truncatula seeds was 55.56%; the germination rate of Medicago truncatula seed removed coat was 100%; water absorption test revealed that Medicago truncatula seed coat hindered embryo absorption to water in a certain extent; with the concentrations of crude extract of Medicago truncatula increasing, the inhibitory effects on the seed germination and seedling growth of Trifolium repens were more obvious. Pre-soaking with 0.1~0.7 mmol·L^-1 GA₃ or SNP promoted seed germination percentage, germination potential, germination index and activity index, in particular, pre-soaking with 0.1 mmol·L^-1 GA₃ or SNP broke seed dormancy significantly. In conclusion, Medicago truncatula seeds are featured multiple dormancies; pre-soaking with GA₃ or SNP solution can break the dormancy of Medicago truncatula effectively.

Key words: Medicago truncatula, Seeds, Dormancy, GA₃, Germination

中图分类号:

Q946.885.5

刘文瑜, 魏小红, 许可成, 赵萌, 段正中. 蒺藜苜蓿种子休眠机制及其破除方法研究[J]. 草地学报, 2015, 23(2): 358-365.

LIU Wen-yu, WEI Xiao-hong, XU Ke-cheng, ZHAO Meng, DUAN Zheng-zhong. Study on Dormancy Property and Breaking Approaches of Medicago truncatula Seed[J]. Acta Agrestia Sinica, 2015, 23(2): 358-365.

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参考文献

[1] 刘建利,云天运,曹君迈,等. 酸处理对蒺藜苜蓿种子萌发的影响[J]. 种子,2008,27(9):87-88
[2] Barker D G, Bianchi S, Blondon F, et al. Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis [J]. Plant Molecular Biology Reporter,1990,8(1):40-49
[3] 陈爱民,连瑞丽,孙杰,等. 豆科模式植物:蒺藜苜蓿[J]. 植物生理学通讯,2006,42(5):997-1003
[4] Graeber K, Linkies A, Müller K. Cross-species approaches to seed dormancy and germination: Conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes [J]. Plant Molecular Biology,2010,73(1/2),67-87
[5] Schütz W. Ecology of seed dormancy and germination in sedges (Carex) [J]. Perspectives in Plant Ecology Evolution Systematics,2000,3(1),67-89
[6] Webb J, Miao S L, Zhang X H. Factors and mechanisms influencing seed germination in a wetland plant sawgrass [J]. Plant Growth Regulation,2009,57(3),243-250
[7] 刘建利. 6-BA和GA₃对蒺藜苜蓿种子萌发的影响[J]. 安徽农业科学,2008,36(17):7103,7115
[8] 刘建利. 7种激素对蒺藜苜蓿种子萌发的影响[J]. 干旱地区农业研究,2010,28(2):113-117
[9] 刘建利. 激素对苜蓿种子萌发的影响[J]. 安徽农业科学,2010,38(2):665-667
[10] 刘建利,任贤,曹君迈,等. 几种激素对蒺藜状苜蓿种子萌发效应的研究[J]. 北方园艺,2008(11):56-57
[11] 刘建利. CO₂对蒺藜苜蓿种子萌发的影响[J]. 种子,2010,28(12):10-12
[12] 陈建勋,王晓峰. 植物生理实验指导[M]. 广州:华南科技大学出版社,2013,48-49
[13] 尹燕坪,董学会. 种子实验技术[M]. 北京:中国农业出版社,2008
[14] 喻梅,周守标,吴晓艳,等. 野生鸭儿芹种子休眠特性及破除方法[J]. 生态学报,2012,32(4):1347-1354
[15] 张恩和,陈小莉,方子森,等. 野生羌活种子休眠机理及破除休眠技术研究[J]. 草地学报,2007,15(6):509-514
[16] 王小雪,孙海菁,刘芸,等. 浓硫酸处理对海滨木槿10个家系种子萌发的影响[J]. 应用生态学报,2012,23(11):2968-2974
[17] 李兵兵,魏小红,徐严. 麻花秦艽种子休眠机理及其破除方法[J]. 生态学报,2013,24(6):1621-1626
[18] 张海燕. 模拟酸雨对不同类型玉米种子萌发和幼苗生长的影响[J]. 应用生态学报,2013,24(6):1621-1626
[19] 张艳杰. 南方红豆杉种子休眠机理的研究[D]. 南京:南京林业大学,2007:31
[20] 杨期和,叶万辉,宋孙泉,等. 植物种子休眠的原因及休眠的多形性[J]. 西北植物学报,2003,23(5):837-843
[21] 潘琳,徐程扬. 种子休眠与萌发过程的生理调控机理[J]. 种子,2010,29(6):42-47
[22] Hilhorsthwm. A critical update on seed dormancy. I. Primary dormancy [J]. Seed Science Research,1995,5(2):61-73
[23] 山东农业大学. 作物种子科学[M]. 郑州:河南科学技术出版社,1988:47
[24] 颜启传. 种子学[M]. 北京:中国农业出版社,2001:64-68
[25] 尚旭岚. 青钱柳种子休眠机理及其解除休眠方法的研究[D]. 南京:南京林业大学,2007:5
[26] 郭继元,彭励,徐青,等. 伏毛铁棒锤种子休眠与破除休眠方法研究[J]. 中国农学通报,2011,27(10):139-143
[27] 李勐,张杰,熊英,等. 穿龙薯蓣种子休眠机理的初步研究[J]. 中国野生植物资源,2009,28(4):48-50,53
[28] Bewley J D. Seed germination and dormancy [J]. Plant Cell,1997,9(7):1055-1066
[29] Koornneef M, Bentsink L, Hilhorst H. Seed dormancy and germination [J]. Current Opinion in Plant Biology,2002,5(1):33-36
[30] Yamaguchi S, Kamiya Y. Gibberellins and light-stimulated seed germination [J]. Journal of Plant Growth Regulation,2002,20(4):369-376
[31] Li W Q, Liu X J, Khan M A, et al. Hormonal and environmental regulation of seed germination in flixweed (Descurainia sophia) [J]. Plant Growth Regulation,2005,45(3):199-207
[32] 周建荣,李仕友. 油松种子休眠特性研究[J]. 阴山学刊,2009,23(1):37-39
[33] 何丽萍,李龙根,庞祖云. 野生泸定百合种子休眠和萌发习性的研究[J]. 种子,2010,29(4):46-49
[34] Bethke P C, Libourel I G L, Jones R L. Nitric oxide reduces seed dormancy in Arabidopsis [J]. Journal of Experimental botany,2006,57(3):517-526
[35] Guo F Q, Crawford N M. Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence [J]. Plant Cell,2005,17(12):3436-3450
[36] He Y K, Tang R H, Yi H, et al. Nitric oxide represses the Arabidopsis floral transition [J]. Science,2004,305(5962):1968-1971
[37] Delledonne M, Xia Y, Dixon R A, et al. Nitric oxide functions as a signal in plant disease resistance [J]. Nature,1998,394(6693):585-588
[38] Zhang H, Shen W B, Zhang W, et al. A rapid response of β:Amylase to nitric oxide but not gibberellin in wheat seeds during the early stage of germination [J]. Planta,2005,220(5):708-716
[39] 刘建新,王鑫,雷蕊霞. 外源一氧化氮供体SNP对黑麦草种子萌发和幼苗生长的影响[J]. 生态学杂志,2007,26(3):393-398
[40] 凌腾芳,宣伟,樊颖瑞,等. 外源葡萄糖、果糖和NO供体(SNP)对盐胁迫下水稻种子萌发的影响[J]. 植物生理与分子生物学学报,2005,31(2):205-212
[41] 张秀玮,董元杰,邱现奎,等. 外源NO 对不同作物种子萌发、幼苗生长及抗氧化酶活性的影响[J]. 植物营养与肥料学报,2012,18(2):397-404

蒺藜苜蓿种子休眠机制及其破除方法研究

Study on Dormancy Property and Breaking Approaches of Medicago truncatula Seed

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