土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响

doi:10.11733/j.issn.1007-0435.2017.04.006

草地学报 ›› 2017, Vol. 25 ›› Issue (4): 724-731,748.DOI: 10.11733/j.issn.1007-0435.2017.04.006

土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响

师生波^1,3, 李天才¹, 王伟², 德科加², 徐先英³, 王祺³, 马剑平³, 李爱德³, 康才周³

1. 中国科学院西北高原生物研究所/高原生物适应与进化重点实验室/青海省作物分子育种重点实验室, 青海西宁 810001;
2. 青海大学/畜牧兽医科学院, 青海西宁 810016;
3. 甘肃省治沙研究所/甘肃省沙生植物工程技术研究中心, 甘肃兰州 730070

收稿日期:2016-06-20 修回日期:2017-06-15 出版日期:2017-08-15 发布日期:2017-11-01
作者简介:师生波(1963-),男,青海西宁人,博士,研究员,主要从事高山植物生理生态适应性研究,E-mail:sbshi@nwipb.cas.cn
基金资助:
青海省重大科技专项“耗牛提质增效技术集成与产业化示范”（2016-NK-A7）；国家自然科学基金项目（31660237）；甘肃省基础研究创新群体项目（1506RJIA155）；青海省自然科学基金面上项目（2016-ZJ-906）；青海省创新平台建设专项项目-青海省作物分子育种重点实验室（2017-ZJ-Y14）资助

Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau

SHI Sheng-bo^1,3, LI Tian-cai¹, WANG Wei², DE Ke-jia², XU Xian-ying³, WANG Qi³, MA Jian-ping³, LI Ai-de³, KANG Cai-zhou³

1. Key Laboratory of Adaptation and Evolution of Plateau Biology/Key Laboratory of Crop Molecular Breeding of Qinghai Province/Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, China;
2. Qinghai Academy of Animal Science and Veterinary Medicine/Qinghai University, Xining, Qinghai Province 810016, China;
3. Research Center of Engineer Technique for Desert Plants in Gansu/Gansu Desert Control Research Institute, Lanzhou, Gansu Province 730070, China

Received:2016-06-20 Revised:2017-06-15 Online:2017-08-15 Published:2017-11-01

摘要/Abstract

摘要：

植物对单一环境因子的响应常会因另一因子存在而被修饰。本文主要采用叶绿素荧光图像分析技术，研究了土壤干旱对高山嵩草（Kobresia pygmaea）叶片光合功能的影响及与强光的交互效应。结果表明：土壤干旱胁迫可引起叶绿素a（Chl a）含量和Chl a/b比值的降低，对总叶绿素（Chl a+b）含量无影响。高光强可导致PSⅡ最大效率（F_v'/F_m'）、PSⅡ运行效率（F_q'/F_m'）和PSⅡ效率因子（F_q'/F_v'）的显著降低，且F_v'/F_m'和F_q'/F_m'的降低为土壤干旱所加剧；非光化学猝灭系数（NPQ）和光化学效率的相对限制（L_（PFD））随光强增大显著增加，也为干旱胁迫所加剧；PSⅡ反应中心开放比率（q_L）随光强的增大而降低，土壤干旱时降低趋势较小。方差分析表明，光强是影响光合机构光能利用和耗散的主要因素；PSⅡ非光化学能量耗散存在着两种胁迫因子的交互效应，但F_q'/F_m'和L_（PFD）则无。证实，高山嵩草能快速响应环境光强的变化，并对土壤干旱胁迫具有较强的耐受性，非光化学能量耗散的加强是光合功能有效维系的保护策略。

关键词: 叶绿素荧光, 青藏高原, 交互效应, 高山嵩草, 土壤干旱胁迫

Abstract:

Plant response to a single environmental factor is often modified by another factor. Kobresia pygmaea was collected from the Alpine Grassland Ecosystem Research Station in the region of Three-River-Source. The chlorophyll fluorescence imaging of leaves was used to determine the interaction effects between soil drought and strong light besides measurement of photosynthetic pigments. The results showed that there was no difference in total chlorophyll contents (Chl a+b), and that chlorophyll a contents (Chl a) and the ratio of chlorophyll a to b (Chl a/b) decreased in soil drought treatment. The PSⅡ maximum and operating efficiency (F_v'/F_m' and F_q'/F_m') and the PSⅡ efficiency factor (F_q'/F_v') were significantly influenced by actinic light intensity, and the decrease of F_v'/F_m' and F_q'/F_m' were intensified by the treatment of soil drought. Non-photochemical quenching coefficient (NPQ) and relative limitation of PSⅡ photochemical efficiency (L_(PFD)) increased with the enhancement of actinic light intensity, and these trends were aggravated in soil drought treatment. GLM univariate analysis indicated that light intensity was the main factor, and excepting F_q'/F_m' and L_(PFD), the fluorescence parameters of NPQ and F_v'/F_m' exhibited interaction effect among actinic light and soil drought treatments. The results demonstrated that K. pygmaea could quickly respond to environmental light variations and had a strong tolerance to soil drought stress. The improvement of dissipation of non-photochemical energy was one of the important strategies for K. pygmaea to maintain effective performance of photosynthetic apparatus in alpine environments.

Key words: Chlorophyll fluorescence, Qinghai-Tibetan Plateau, Interaction effect, Kobresia pygmaea, Soil drought stress

中图分类号:

Q945.11

师生波, 李天才, 王伟, 德科加, 徐先英, 王祺, 马剑平, 李爱德, 康才周. 土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响[J]. 草地学报, 2017, 25(4): 724-731,748.

SHI Sheng-bo, LI Tian-cai, WANG Wei, DE Ke-jia, XU Xian-ying, WANG Qi, MA Jian-ping, LI Ai-de, KANG Cai-zhou. Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau[J]. Acta Agrestia Sinica, 2017, 25(4): 724-731,748.

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土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响

Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau

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