山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定

doi:10.11733/j.issn.1007-0435.2021.06.027

草地学报 ›› 2021, Vol. 29 ›› Issue (6): 1350-1356.DOI: 10.11733/j.issn.1007-0435.2021.06.027

山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定

邢会琴¹, 王春明², 金社林², 周天旺², 郭成²

1. 河西学院农业与生态工程学院, 甘肃张掖 734000;
2. 甘肃省农业科学院植物保护研究所, 甘肃兰州 730070

收稿日期:2020-12-12 修回日期:2021-01-26 出版日期:2021-06-15 发布日期:2021-07-05
通讯作者: 王春明,E-mail:wchm179@126.com;金社林,E-mail:jinshelin@163.com
作者简介:邢会琴(1974-)，女，甘肃山丹人，博士，副教授，主要从事植物病理学教学与科学研究，E-mail:gszyxhq@163.com
基金资助:
绿肥产业技术体系旱地病虫害综合防控岗位（CARS-22-G-20）；甘肃省科技计划（20JR5RA108）；嘉峪关市科技计划（20-17）资助

Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot

XING Hui-qin¹, WANG Chun-ming², JIN She-lin², ZHOU Tian-wang², GUO Cheng²

1. College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu Province 734000, China;
2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China

Received:2020-12-12 Revised:2021-01-26 Online:2021-06-15 Published:2021-07-05

摘要/Abstract

摘要： 为了明确山黧豆（Lathyrus sativus）根腐病病原菌的分类地位，本研究于2018年8月在四川省南充市采集山黧豆根腐病病样，经组织分离法进行分离纯化，采用形态学和翻译延伸因子（Translation elongation factor 1-agene，EF-1α）基因序列分析相结合的方法对致病菌进行鉴定。结果表明：从采集的山黧豆根腐病病样上共分离获得39株分离物，其中3株分离物Shan-27，Shan-28和Shan-29对山黧豆具有强致病性，分离频率为7.69%；这3株致病菌在形态学上均接近于禾谷镰孢菌复合种（Fusarium graminearum species complex），而对其翻译延伸因子（EF-1α）序列分析发现，其均与亚洲镰孢菌聚在同一分支上，同源性达99%以上。因此，根据菌株形态学特征和EF-1α基因序列分析结果，将引致山黧豆根腐病的病原菌确定为亚洲镰孢菌（Fusarium asiaticum）。

关键词: 山黧豆, 根腐病, 亚洲镰孢菌, 病原鉴定, EF-1α

Abstract: In order to identify the pathogen causing root rot on Lathyrus sativus,the infected plants were collected during August of 2018 in Nanchong city,Sichuan province. The pathogen was isolated from tissue， single spore was purified,and pathogenicity was tested in the laboratory. Pathogenic isolates were identified by morphological characteristics and EF-1α gene sequence analysis. The results showed that 39 isolates were obtained from infected plant,of which three isolates including Shan-27,Shan-28 and Shan-29 had strong pathogenicity to L.sativus,accounting for 7.69%. The above three strains were all close to Fusarium graminearum species complex in morphology. Their translation elongation factor (EF-1α) were amplified by PCR,sequenced,and compared with Blast in GenBank. The results showed that their EF-1α were clustered in the same group with F. asiaticum, and their homology were more than 99%. Therefore,according to the morphological characteristics of the isolates and the sequence analysis of EF-1α gene,the three pathogenic isolates causing L.sativus root rot were identified as F. asiaticum.

Key words: Lathyrus sativus, Root rot, Fusarium asiaticum, Identification of pathogen, Translation elongation factor

中图分类号:

S432.4

邢会琴, 王春明, 金社林, 周天旺, 郭成. 山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定[J]. 草地学报, 2021, 29(6): 1350-1356.

XING Hui-qin, WANG Chun-ming, JIN She-lin, ZHOU Tian-wang, GUO Cheng. Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot[J]. Acta Agrestia Sinica, 2021, 29(6): 1350-1356.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.06.027

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I6/1350

参考文献

[1] 熊俊兰,白雪,Asfa Batool,等. 山黧豆毒素β-ODAP的生态学功能及应用[J]. 应用生态学报,2014,25(4):1197-1205
[2] Girma D,Korbu L. Genetic improvement of grass pea (Lathyrus sativus) in Ethiopia:An unfulfilled promise[J]. Plant Breeding,2012,131(2):231-236
[3] 董滢,周庆安. 山黧豆研究进展[J]. 饲料工业,2005,26(3):50-53
[4] 曲瑞红,刘凤娟,毕春晓,等. 山黧豆种子萌发特异性蛋白酶的分离纯化及酶学性质研究[J]. 草地学报,2018,26(6):1497-1502
[5] Hanbury C D,Siddique K H M,Galwey N W,et al. Genotype-environment interaction for seed yield and ODAP concentration of Lathyrus sativus L. and L. cicera L. in Mediterranean-type environments[J]. Euphytica,1999,110:45-60
[6] Calderón F J,Vigil M F,Nielsen D C,et al. Water use and yields of no-till managed dryland grasspea and yellow pea under different planting configurations[J]. Field Crops Research,2012,125:179-185
[7] Campbell C G,Mehra R B,Agrawal S K,et al. Current status and future strategy in breeding grasspea (Lathyrus sativus)[J]. Euphytica,1994,73(1):167-175
[8] 张明科,刘凤娟,陶英杰,等. 山黧豆CASase基因的克隆及RNAi载体的构建[J]. 草地学报,2016,24(5):1146-1149
[9] Vaz Patto M C,Skiba B,Pang E C K,et al. Lathyrus improvement for resistance against biotic and abiotic stresses:From classical breeding to marker assisted selection[J]. Euphytica,2006,147(1):133-147
[10] 张大伟,邢更妹,熊友才,等. 山黧豆毒素ODAP的生物合成及与抗逆性关系研究进展[J]. 生态学报,2011,31(9):2621-2630
[11] 董晓霞,刘兆辉,李志禄,等. 豆科牧草对滨海盐渍土壤盐分特性和肥力影响的研究[J]. 安徽农业科学,2008,36(14):6060-6062
[12] 方中达. 植病研究方法[M]. 北京:中国农业出版社,1998:122-124
[13] Burgess L W,Summerell B A,Bullock A,et al. Laboratory mannual for Fusarium research[M]. Sydney:The University of Sydney,1994:12-16
[14] 中华人民共和国农业部.NY/T1248.7-2016.玉米抗病虫性鉴定技术规范第7部分:镰孢茎腐病[S]. 北京:中国标准出版社,2016:1-4
[15] Leslie J F,Summerell B A. The Fusarium laboratory manual[M]. New Jersey:Blackwell Publishing,2006:121-278
[16] Irina S D,Alexei G K,Monika K. An oligonucleotide barcode for species identification in Trichoderma and Hypocrea[J]. Fungal Genetics and Biology,2005,42(10):813-828
[17] Geiser D M,Lewis-Ivey M L,Hakiza Q,et al. Gibberella xylarioides(anamorph Fusarium xylarioides),a causative agent of coffee wilt disease in Africa,is a previously unrecognized member of the G.fujikuroi species complex[J]. Mycologia,2005,97(1):191-201
[18] 王春华,孙超,刘淑燕. 新疆花芸豆根腐病病原及防治的初步研究[J]. 干旱区研究,2010,27(3):380-384
[19] 郑培娥. 大豆根腐病多种病原菌的分子检测及复合侵染规律研究[D]. 乌鲁木齐:新疆农业大学,2012:11-16
[20] 吴仁锋,杨绍丽,万鹏,等. 豇豆根腐病病原分离鉴定及其核糖体rDNA-ITS序列分析[J]. 湖北农业科学,2011,50(24):5107-5110
[21] 张河庆,席亚东,陈玲,等. 一种新的豇豆根腐病病原菌鉴定及室内药剂筛选[J]. 植物保护,2018,44(2):177-183
[22] 杨帅,王健生,马振川,等. 引致大豆根腐病的层出镰孢菌分离鉴定及其特性[J]. 植物保护学报,2012,39(2):187-188
[23] 闫文雪,石延霞,柴阿丽,等. 豇豆根腐病病原菌鉴定[J]. 植物保护学报,2019,46(3):717-718
[24] 从丽丽,康俊梅,张铁军,等. 苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J]. 草地学报,2017,25(4):857-865
[25] Suleiman M N,Emua S A. Efficacy of four plant extracts in the control of root rot disease of cowpea (Vigna unguiculata[L.] Walp)[J]. African Journal of Biotechnology,2009,8(16):3806-3808
[26] Satish L,Uday B,Lodha S,et al. Efficacy of composts on nitrogen fixation,dry root rot (Macrophomina phaseolina) intensity and yield of legumes[J]. Indian Journal of Agriculture Science,2000,70(12):846-849
[27] 罗舜文. 亚洲镰孢菌(Fusarium asiaticum)β-微管蛋白基因的点突变及其内含子对多菌灵药敏性的调控机制研究[D]. 南京:南京农业大学,2018:1-4
[28] 高先悦,高淑敏,刘少杰,等. 山东省小麦赤霉病菌的种群组成及毒素化学型分析[J]. 华北农学报,2017,32(增刊):37-42
[29] 王宝宝,毕四刚,肖明纲,等. 黑龙江省玉米穗腐病致病镰孢菌分离鉴定及产毒基因型分析[J]. 草业学报,2020,29(1):163-174

山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定

Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

[1]	徐美蓉, 李敏权, 曹素芳, 蒋晶晶, 李雪萍, 孙新荣, 陈爱昌, 李继平, 漆永红. 根腐病对党参细胞结构和生理特性的影响[J]. 草地学报, 2020, 28(4): 915-922.
[2]	曾亮, 柴继宽, 赵桂琴, 李春杰, 刘文辉, 姚拓. 苜蓿根腐病生防真菌筛选鉴定及其防治效果研究[J]. 草地学报, 2019, 27(4): 825-831.
[3]	曲瑞红, 刘凤娟, 毕春晓, 宋瑶瑶, 胡鑫, 徐全乐. 山黧豆种子萌发特异性蛋白酶的分离纯化及酶学性质研究[J]. 草地学报, 2018, 26(6): 1497-1502.
[4]	漆永红, 曹素芳, 李雪萍, 李敏权. 甘南州临潭县青稞根际土壤养分含量、酶活性和微生物数量与根腐病的关系研究[J]. 草地学报, 2018, 26(4): 877-884.
[5]	丛丽丽, 康俊梅, 张铁军, 龙瑞才, 杨青川. 苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J]. 草地学报, 2017, 25(4): 857-865.
[6]	张明科, 刘凤娟, 陶英杰, 胡鑫, 李荣硕, 徐全乐. 山黧豆CASase基因的克隆及RNAi载体的构建[J]. 草地学报, 2016, 24(5): 1146-1149.
[7]	曾翠云, 朱晓玉, 崔振, 李彦忠. 药用植物提取液对沙打旺黄矮根腐病菌的抑菌活性及对其种子萌发的影响[J]. 草地学报, 2015, 23(2): 366-371.
[8]	王歧, 段碧华, 杨瑞, 赵耀荣, 张露. 产几丁质酶放线菌的筛选、鉴定及其对草坪草根腐病真菌的抑制作用[J]. 草地学报, 2013, 21(5): 998-1004.
[9]	郑莉, 黄俊斌, 向培建. 高羊茅叶斑病病原鉴定及生物学特性[J]. , 2006, 14(4): 315-318,327.
[10]	雷玉明, 张建文. 草本植物三种真菌新病害[J]. , 2006, 14(3): 284-286.
[11]	曾翠云, 何冬云, 王生荣. 西藏牧草黑粉病及其病原鉴定[J]. , 2006, 14(2): 181-183.
[12]	周洪友, 杨合同, 唐文华. 沙打旺根腐病发生及病原菌鉴定[J]. , 2004, 12(4): 285-288,297.