草地学报

草地学报 ›› 2013, Vol. 21 ›› Issue (5): 998-1004.DOI: 10.11733/j.issn.1007-0435.2013.05.025

• 研究论文 • 上一篇    下一篇

产几丁质酶放线菌的筛选、鉴定及其对草坪草根腐病真菌的抑制作用

王歧1, 段碧华2, 杨瑞2, 赵耀荣1, 张露1   

  1. 1. 北京农学院园林学院, 北京 102206;
    2. 北京农学院植物科学技术学院 农业应用新技术北京市重点实验室, 北京 102206
  • 收稿日期:2013-03-07 修回日期:2013-04-19 出版日期:2013-10-15 发布日期:2013-10-30
  • 通讯作者: 段碧华
  • 作者简介:王歧(1987- ),男,吉林磐石人,硕士研究生,研究方向为生物防治, E-mail: leibiyijue@126.com
  • 基金资助:

    国家自然科学基金(31000551)(3097012)资助

Screening and Identification of Chitinolytic Actinomycetes and the Inhibitory Activity on Turfgrass Root Rot Disease Fungi

WANG Qi1, DUAN Bi-hua2, YANG Rui2, ZHAO Yao-rong1, ZHANG Lu1   

  1. 1. College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China;
    2. College of Plant Science and Technology, Beijing University of Agriculture, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206, China
  • Received:2013-03-07 Revised:2013-04-19 Online:2013-10-15 Published:2013-10-30

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摘要： 通过对草坪土壤中产几丁质酶放线菌的筛选、鉴定及对4种草坪草根腐病菌的抑制作用研究,为草坪草根腐病的生物防治提供指导。通过菌落法和菌饼法筛选得到1株产几丁质酶活性较强的放线菌菌株Bn035,其降解圈相对直径分别为3.60和3.42;采用DNS法测量几丁质酶活性发现,第6 d时酶活达到最大为1.23 U·mL-1;菌体和粗酶液均对草坪草根腐病菌产生较强的抑制作用;根据形态学特征、培养特征、生理生化特征和16S rDNA序列比对综合分析,菌株Bn035为皱孢链霉菌(Streptomyces scabrisporus)。菌株Bn035产几丁质酶活性较强且抑菌效果较好,表明其在真菌性根腐病生物防治方面具有潜力。

关键词: 几丁质酶, 放线菌, 草坪草, 根腐病, 筛选, 鉴定, 抑制作用

Abstract: Chitinolytic actinomycetes from lawn soil were screened and identified and their inhibitory activities on four types of turfgrass root rot disease were studied. Results showed that screened Bn035 strain had stronger chitinase activity than the other four strains; the relative diameter of chitin-lytic zone was 3.60 and 3.42, respectively. Its chitinase activity in culture broth reached a maximum value of 1.23 U·mL-1 on the 6th day. Crude enzyme solution and thallus of Bn035 strain showed stronger antifungal activity against all tested fungi. The Bn035 strain was identified as Streptomyces scabrisporus by morphological, cultural, physiological, biochemical and phylogenetic characteristics. The Bn035 strain had strong chitinase activity and inhibitory activity that suggested its potential application in the biological control of turfgrass fungal root rot disease.

Key words: Chitinase, Actinomycetes, Turfgrass, Root rot disease, Screening, Identification, Inhibition

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