紫花苜蓿叶枯病病原菌鉴定、生物学特性及防治药剂筛选

doi:10.11733/j.issn.1007-0435.2024.11.006

草地学报 ›› 2024, Vol. 32 ›› Issue (11): 3391-3399.DOI: 10.11733/j.issn.1007-0435.2024.11.006

• 研究论文 • 上一篇

紫花苜蓿叶枯病病原菌鉴定、生物学特性及防治药剂筛选

肖钰莹, 木扎帕尔·吐鲁洪, 徐文, 王柏吉, 刘千宁, 张庆雯, 崔国文

东北农业大学动物科学技术学院, 黑龙江哈尔滨 150036

收稿日期:2024-02-22 修回日期:2024-04-07 发布日期:2024-11-29
通讯作者: 崔国文,E-mail:cgw603@163.com
作者简介:肖钰莹(2001-),女,汉族,黑龙江鹤岗人,硕士研究生,主要从事牧草抗病育种研究,E-mail:18846803061@163.com
基金资助:
耐寒高产苜蓿育种技术创新及新种源创制(2022ZD040120401)资助

Identification, Biological Characteristics and Screening of Control Agents of the Medicago sativa Leaf Blight

XIAO Yu-ying, TULUHONG Mu-zhapaer, XU Wen, WANG Bai-ji, LIU Qian-ning, ZHANG Qing-wen, CUI Guo-wen

College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province 150036, China

Received:2024-02-22 Revised:2024-04-07 Published:2024-11-29

摘要/Abstract

摘要： 为明确紫花苜蓿(Medicago sativa L.) 叶枯病病原菌种类及生物学特性，筛选出有效防治药剂，本试验于2023年8月在黑龙江省肇东市和大庆市分别采集紫花苜蓿叶枯病病样，采用组织分离法分离并纯化病原菌，依据柯赫氏法则验证致病性，通过菌丝生长速率法进行室内药剂筛选。形态学结合分子生物学分析结果表明，引起紫花苜蓿叶枯病的病原菌为黑附球菌(Epicoccum nigrum)。生物学特性研究结果表明，菌丝生长最适温度25℃、最适pH值为10，全光照条件有利于菌丝生长，菌丝在马铃薯蔗糖琼脂(Potato saccharose agar，PSA)培养基上生长速度最快，在水琼脂(Water agar，WA)培养基上生长速度最慢。碳源和氮源选择上，菌丝分别在麦芽糖和磷酸二氢铵上生长速度最快，在蔗糖和硝酸钾上生长速度最慢。室内药剂毒力测定表明，30%吡唑醚菌酯抑菌作用最强，半数效应浓度(Median effect concentration，EC₅₀)为0.2819 μg·mL^-1。

关键词: 紫花苜蓿, 黑附球菌, 病原菌鉴定, 生物学特性, 药剂毒力测定

Abstract: To clarify the species and biological characteristics of pathogenic bacteria of Medicago sativa L. leaf blight,and screen out the effective fungicides to control this disease. Leaf samples of alfalfa leaf blight were collected in Zhaodong City and Daqing City,Heilongjiang Province in August,2023. Tissue isolation method was used to isolate and purify pathogenic bacteria,according to Koch’s rule to verify pathogenicity,and the mycelium growth in plate was used to investigate fungicides toxicity on the pathogen. Combined analyses of morphology and molecular biology indicated that the pathogen causing alfalfa leaf blight was Epicoccum nigrum. The results of studied biological characteristics showed that the optimum temperature for mycelial growth was 25℃,which was favorable for mycelial growth under all light conditions and pH value was 10. The mycelium growth rate was the fastest on PSA medium,but the growth rate was the slowest on WA medium. In the choice of carbon source,mycelium grew fastest on maltose and slowest on glycerol. In the choice of nitrogen source,the mycelium grew fastest on tryptone and slowest on urea. The indoor toxicity test showed that 30% pyraclostrobin had the strongest inhibitory effect,and the EC₅₀ value was 0.2819 μg·mL^-1.

Key words: Medicago sativa, Epicoccum nigrum, Pathogen identification, Biological characteristics, Toxicity test

中图分类号:

S435.4

肖钰莹, 木扎帕尔·吐鲁洪, 徐文, 王柏吉, 刘千宁, 张庆雯, 崔国文. 紫花苜蓿叶枯病病原菌鉴定、生物学特性及防治药剂筛选[J]. 草地学报, 2024, 32(11): 3391-3399.

XIAO Yu-ying, TULUHONG Mu-zhapaer, XU Wen, WANG Bai-ji, LIU Qian-ning, ZHANG Qing-wen, CUI Guo-wen. Identification, Biological Characteristics and Screening of Control Agents of the Medicago sativa Leaf Blight[J]. Acta Agrestia Sinica, 2024, 32(11): 3391-3399.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2024.11.006

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2024/V32/I11/3391

参考文献

[1] 段代祥,赵丽萍,赵凤娟,等. 苜蓿青贮在牛羊饲料中的应用[J]. 饲料研究,2023,46(20):169-172
[2] 张春梅,王成章,胡喜峰,等. 紫花苜蓿的营养价值及应用研究进展[J]. 中国饲料,2005(1):15-17
[3] 袁庆华. 我国苜蓿病害研究进展[J]. 植物保护,2007,33(1):6-10
[4] 陈婧,郭子雯,潘春清,等. 苜蓿病虫草害研究现状[J]. 草学,2022(1):1-14
[5] XU S,LI Y Z,NAN Z B. First report of Verticillium wilt of alfalfa caused by Verticillium alfalfae in China[J]. Plant Disease,2016,100(1):220
[6] 袁玉涛,史娟,马新,等. 紫花苜蓿白粉病病原菌鉴定及其生物学特性[J]. 微生物学通报,2020,47(11):3539-3550
[7] 杨庆森,汤春梅,蔡继增. 苜蓿霜霉病病原及其生物学特性研究[J]. 安徽农业科学,2010,38(1):207-208
[8] 李杨,史娟,崔娜娜,等. 苜蓿褐斑病对紫花苜蓿光合作用及草品质的影响[J]. 草业学报,2017,26(10):149-157
[9] 韩帅,张河庆,吴婕,等. 四川省山药黑斑病病原菌的鉴定[J]. 植物保护,2019,45(2):68-74
[10] 李扬,王亚南,胡同乐,等. 黑附球菌在植物病害生物防治中的研究与应用进展[J]. 安徽农业科学,2010,38(6):2988-2990
[11] LARENA I,DE CAL A,MELGAREJO P. Solid substrate production of Epicoccum nigrum conidia for biological control of brown rot on stone fruits[J]. International Journal of Food Microbiology,2004,94(2):161-167
[12] 夏丽琴,王亚南,苏艳超,等. 黑附球菌XF₁菌株分生孢子产生和保存条件研究[J]. 河北农业大学学报,2012,35(4):75-79
[13] 李扬. 黑附球菌XF1菌株对马铃薯晚疫病的生防作用[D]. 保定:河北农业大学,2010:21-22
[14] 台莲梅,姜小玉,靳学慧,等. 黑龙江省水稻穗褐变病病原菌的分离与鉴定[J]. 微生物学通报,2020,47(6):1776-1786
[15] COLAVOLPE B,EZQUIAGA J,MAIALE S,et al. First report of Epicoccum nigrum causing disease in Lotus corniculatus in Argentina[J]. New Disease Reports,2018,38(1):6
[16] 王瑜,袁庆华,苗丽宏,等. 东北与华北地区紫花苜蓿病害调查与主要病害流行规律研究[J]. 草业学报,2016,25(3):52-59
[17] 沈宝宇,李旭,李维根,等. 辽五味子叶枯病防治药剂筛选[J]. 农药,2023,62(10):763-765,770
[18] 任晓凤,刘苗,周倩,等. 青风藤叶枯病病原菌的分离鉴定及防治药剂的筛选[J]. 湖南农业大学学报(自然科学版),2023,49(1):74-78
[19] 方中达. 植病研究方法[M]. 第三版.北京:中国农业出版社,1998:122-154
[20] 邱小燕,汤智鹏,张敏,等. 一种适用于多数植物病原真菌的单孢分离方法[J]. 安徽农业科学,2011,39(9):5263-5264
[21] 薛婧,侯学萍,姜晓东,等. 藜麦炭疽病病原菌鉴定、生物学特性及室内药剂毒力测定[J/OL]. https://doi.org/10.13926/j.cnki.apps.001638,2024-01-30/2024-05-12
[22] WANG L,LIN K,LIU H,et al. First report of leaf spot disease caused by on smooth bromegrass (Bromus inermis) in China[J]. Plant Disease,2023,107(8):2279-2571
[23] JIN M J,YANG C D,WANG Y D,et al. Isolation and identification of Epicoccum nigrum as the causal agent of brown spot disease in Solanum tuberosum in China[J]. Plant Pathology,2023,72(4):829-838
[24] 张天宁. 中国真菌志[M]. 北京:科学出版社,2009:64-66
[25] CAI Y B,DOU T,GAO F T,et al. Identification and biological characteristics of a pathogen causing leaf blight of Rehmannia glutinosa[J]. China Journal of Chinese Materia Medica,2022,47(7):1824-1830
[26] 梁嘉俊,李芳红,高鸿鹏,等. 杨凌区紫花苜蓿叶枯病病原分离鉴定及其生物学特性[J]. 草地学报,2018,26(5):1208-1214
[27] 孙雪艳,蒋晶晶,杜蕙,等. 甘肃陇西黄芩灰霉病病原菌分离鉴定及田间药剂防治[J]. 草地学报,2023,31(2):349-357
[28] 马桂花,段晓明,徐文华,等. 蒙古黄芪根腐病病原鉴定及防治药剂室内筛选[J]. 草地学报,2022,30(5):1122-1130
[29] QADER M M,HAMED A A,SOLDATOU S,et al. Antimicrobial and antibiofilm activities of the fungal metabolites isolated from the marine endophytes Epicoccum nigrum M13 and Alternaria alternata 13A[J]. Marine Drugs,2021,19(4):232
[30] YIN Q X,JIANG S L,LI D X,et al. First report of Epicoccum nigrum causing brown leaf spot in tea in Guizhou Province,China[J]. Plant Disease,2022,106(1):321
[31] DEEPIKA Y S,MAHADEVAKUMAR S,AMRUTHESH K N,et al. First report of Epicoccum nigrum associated with leaf spot disease of cowpea (Vigna unguiculata) from India[J]. Journal of Plant Pathology,2021,103(1):391-392
[32] KOU Z A,ZHANG Z K,ZHANG W X,et al. First report of Epicoccum nigrum causing leaf spot of endive (Cichorium endivia L.) in China[J]. Crop Protection,2024,177:106534
[33] BERNARDI C,REY M S,JUNIOR A W,et al. First report of Epicoccum nigrum causing leaf spot of Eugenia involucrata in Brazil[J]. Plant Disease,2023,107(1):230
[34] BERNARDI C, REY M D S,WAGNER A Jr,et al. First report of Epicoccum nigrum causing leaf spots on Campomanesia guazumifolia (Camb.) Berg in Brazil[J]. Plant Disease,2024,108(1):214
[35] MAHADEVAKUMAR S,JAYARAMAIAH K M,JANARDH-ANA G R. First report of leaf spot disease caused by Epicoccum nigrum on Lablab purpureus in India[J]. Plant Disease,2014,98(2):284
[36] 谭万忠. 论作物病害田间诊断与实验室检验原理和技术[C]//重庆市植物保护学会. 庆祝重庆市植物保护学会成立10周年暨植保科技论坛论文集.重庆:中国农业科学技术出版社,2007:41-48
[37] WU D,ZHANG D H,TIMKO M P,et al. First report of Epicoccum nigrum causing brown leaf spot of loquat in Southwestern China[J]. Plant Disease,2017,101(8):1553
[38] 王俊丽,任建国,刘红美. 一种引起火龙果茎病害的病原菌鉴定[J]. 中国农学通报,2014,30(10):307-311
[39] KILPATRICK J A,CHILVERS G A. Variation in a natural population of Epicoccum purpurascens[J]. Transactions of the British Mycological Society,1981,77(3):497-508
[40] ARENAL F,PLATAS G,MARTÍN J,et al. Evaluation of different PCR-based DNA fingerprinting techniques for assessing the genetic variability of isolates of the fungus Epicoccum nigrum[J]. Journal of Applied Microbiology,1999,87(6):898-906
[41] 闫浩浩. 黑龙江省蓝靛果叶斑病病原黑附球菌Epicoccum nigrum分离鉴定及生物学特性[J/OL]. https://doi.org/10.13926/j.cnki.apps.000903,2023-12-02/2024-05-12
[42] 李柘柘,崔凌霄,魏立娟,等. 云杉叶枯病新病原的鉴定及其生物学特性测定[J]. 西北农业学报,2023,32(5):791-798
[43] 陈仕江. 四川大蒜叶枯病病原、流行动态及防治药剂研究[D]. 雅安:四川农业大学,2013:27-30
[44] 安可良. 日照市苹果炭疽菌叶枯病发生情况及防治[J]. 农技服务,2014,31(6):164-165

紫花苜蓿叶枯病病原菌鉴定、生物学特性及防治药剂筛选

Identification, Biological Characteristics and Screening of Control Agents of the Medicago sativa Leaf Blight

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	武欣, 张芮, 李妙祺, 张小艳, 张真荣, 卢小波. 紫花苜蓿亏缺灌溉栽培的综合管理策略研究[J]. 草地学报, 2024, 32(9): 2677-2685.
[2]	贾雪, 蔡回彩, 于澜, 李沛, 徐博. 紫花苜蓿不育系与保持系线粒体全基因组比较分析[J]. 草地学报, 2024, 32(9): 2737-2748.
[3]	苏小港, 额尔德木图, 成蓉蓉, 陈艳红, 巩泽琳, 张晓琳, 翟鹏辉. 增水和施肥对苜蓿-小麦轮作系统冬小麦叶片水分利用效率的影响[J]. 草地学报, 2024, 32(9): 2891-2898.
[4]	陈辰, 滑少波, 李杰勤, 姜华, 黄鹤平, 姜婉琦, 姚敏学, 李向飞, 初晓辉, 刘秦华. 紫花苜蓿青贮饲料对草鱼生长性能和肌肉品质的影响[J]. 草地学报, 2024, 32(9): 2990-2998.
[5]	徐明, 蒋学乾, 何飞, 张帆, 杨天辉, 龙瑞才, 杨青川, 丛丽丽. 紫花苜蓿种子产量与大小相关性状全基因组关联分析[J]. 草地学报, 2024, 32(8): 2419-2427.
[6]	邹苇鹏, 翟佳兴, 李迪娜, 黄洁琼, 郭康杰, 岑慧芳, 朱慧森, 许涛. 紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析[J]. 草地学报, 2024, 32(8): 2440-2458.
[7]	李婕娜, 冯甘霖, 庞晓攀, 魏小星, 刘慧霞. 水磷配施对紫花苜蓿叶片特征及氮磷计量比的影响[J]. 草地学报, 2024, 32(8): 2484-2497.
[8]	黄洁琼, 翟佳兴, 王颖, 岑慧芳, 朱慧森, 许涛, 夏方山. 褪黑素对干旱胁迫下紫花苜蓿种子萌发及幼苗生理特性的影响[J]. 草地学报, 2024, 32(8): 2575-2583.
[9]	刘泽宇, 袁梓桐, 隋可, 张玉霞, 刘庭玉, 单新河, 张智勇, 王显国. 基于抗寒系数对“WL”系列苜蓿品种抗寒性的综合评价[J]. 草地学报, 2024, 32(8): 2592-2598.
[10]	陈露, 刘佳欣, 李思宇, 陈宁, 包凤轩, 王凤, 曲善民, 刘国富. 耐盐碱根际促生细菌对盐碱胁迫下紫花苜蓿生长和根系生理特性的影响[J]. 草地学报, 2024, 32(7): 2323-2329.
[11]	王之杰, 李明序, 张万军. 根癌农杆菌介导的紫花苜蓿遗传转化体系的优化[J]. 草地学报, 2024, 32(6): 1665-1671.
[12]	代先林, 马晓颖, 仝宗永, 王瑜, 李向林, 柴旭旭, 何峰. 羊尿氮对苜蓿和高羊茅混播草地生物量和氮利用的影响[J]. 草地学报, 2024, 32(6): 1864-1871.
[13]	王龙然, 王伟, 蒲小剑, 魏希杰, 傅云洁, 徐成体. 青海东部干旱区不同紫花苜蓿品种饲用品质和生产性能比较[J]. 草地学报, 2024, 32(6): 1936-1943.
[14]	匡倩, 宗亚倩, 杨文, 黄鹤平, 何承刚, 姜华. 空间诱变对‘德钦’紫花苜蓿农艺性状和营养品质的影响[J]. 草地学报, 2024, 32(6): 1962-1967.
[15]	孙铁男, 李茂娜, 陈芬, 张玉霞, 王显国. 不同灌溉下限对科尔沁沙地紫花苜蓿生产性能的影响[J]. 草地学报, 2024, 32(6): 1968-1973.