四倍体‘紫光’狼尾草的诱导及鉴定分析

doi:10.11733/j.issn.1007-0435.2025.10.030

草地学报 ›› 2025, Vol. 33 ›› Issue (10): 3430-3437.DOI: 10.11733/j.issn.1007-0435.2025.10.030

• 技术研发 • 上一篇

四倍体‘紫光’狼尾草的诱导及鉴定分析

党佳旗^1,2, 王鑫², 苏妮妮², 郭一荻², 纪佳慧², 刘凌云², 滕珂², 穆娜², 岳跃森², 范希峰²

1. 北京农学院园林学院, 北京 102206;
2. 北京市农林科学院草业花卉与景观生态研究所, 北京 100097

收稿日期:2025-05-12 修回日期:2025-08-21 发布日期:2025-10-17
通讯作者: 范希峰,E-mail:fanxifeng@baafs.net.cn
作者简介:范希峰,E-mail:fanxifeng@baafs.net.cn
基金资助:
北京市农林科学院科技创新能力建设专项（KJCX20251208）；北京市农林科学院科技创新能力建设专项（KJCX20230119）；云南省科技计划项目（202403AP140045）资助

Induction and Identification Analysis of Tetraploid Pennisetum alopecuroides ‘Ziguang’

DANG Jia-qi^1,2, WANG Xin², SU Ni-ni², GUO Yi-di², JI Jia-hui², LIU Ling-yun², TENG Ke², MU Na², YUE Yue-sen², FAN Xi-feng²

1. College of Landscape and Architecture, Beijing University of Agriculture, Beijing 102206, China;
2. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2025-05-12 Revised:2025-08-21 Published:2025-10-17

摘要/Abstract

摘要： 为获得‘紫光’狼尾草（Pennisetum alopecuroides ‘Ziguang’）四倍体植株并筛选最适诱导条件，本研究以‘紫光’狼尾草幼穗为试验材料，以其诱导出的胚性愈伤组织为外植体，采用秋水仙素浸渍法对胚性愈伤组织进行加倍处理，组织培养后获得再生植株。分别通过流式细胞术鉴定、根尖染色体计数法鉴定、气孔特征分析鉴定出诱导成功的四倍体植株，并对二倍体和四倍体植株的表型特征进行比对。结果表明：在秋水仙素浓度为0.10%，处理时间为48 h的条件下诱导率最高，可达55.56%；流式细胞术显示，四倍体植株的DNA相对含量为二倍体植株的2倍；根尖染色体计数法鉴定到四倍体植株（2n=4x=36）的根尖染色体数目是二倍体植株（2n=2x=18）染色体数目的2倍；通过气孔鉴定和植株表型特征比较，四倍体植株相较二倍体植株表现为叶宽增大、株高降低、气孔增大、气孔密度降低等特点。

关键词: ‘紫光’狼尾草, 秋水仙素, 多倍体诱导, 倍性鉴定

Abstract: In order to obtain tetraploid plants of Pennisetum alopecuroides ‘Ziguang’ and explore the optimal induction conditions， the young-ear of P.alopecuroides ‘Ziguang’ was used as material， and the induced embryogenic callus was used as explants in this study. The colchicine dipping method was used to induce mutagenesis of P. alopecuroides ‘Ziguang’.The induced tetraploid plants were identified through flow cytometry method （FCM） and root tip chromosome counting， and leaf stomatal characteristics and the phenotypic characteristics were compared between diploid and tetraploid plants. The results showed that the induction rate （55.56%） was the highest when the colchicine concentration was 0.10% and the treatment time was 48 hours.The results of FCM showed that the cellular DNA content of the induced tetraploid strain was doubled compared with that of the diploid plants. The root tip chromosome counting method found that the induced tetraploid plants（2n=4x=36） was twice the number of chromosomes in diploid plants （2n=2x=18）.Through stomatal identification and comparison of plant phenotypic characteristics， tetraploid plants showed increased leaf width， decreased plant height， increased stomata， and decreased stomatal density compared with diploid plants.

Key words: Pennisetum alopecuroides ‘Ziguang’, Colchicine, Polyploidy induction, Ploidy identification

中图分类号:

党佳旗, 王鑫, 苏妮妮, 郭一荻, 纪佳慧, 刘凌云, 滕珂, 穆娜, 岳跃森, 范希峰. 四倍体‘紫光’狼尾草的诱导及鉴定分析[J]. 草地学报, 2025, 33(10): 3430-3437.

DANG Jia-qi, WANG Xin, SU Ni-ni, GUO Yi-di, JI Jia-hui, LIU Ling-yun, TENG Ke, MU Na, YUE Yue-sen, FAN Xi-feng. Induction and Identification Analysis of Tetraploid Pennisetum alopecuroides ‘Ziguang’[J]. Acta Agrestia Sinica, 2025, 33(10): 3430-3437.

参考文献

[1] 杨春华，张建，张璐. 观赏草资源学[M]. 北京:中国林业出版社，2015:4
[2] WU J Y. Ornamental grasses:injecting new vitality into garden design[J]. China Flowers Horticulture，2003（15）:38-39 武菊英. 观赏草:为花园设计注入新的活力[J]. 中国花卉园艺，2003（15）:38-39
[3] 刘建秀. 草坪·地被植物·观赏草[M]. 南京:东南大学出版社，2011:117
[4] YANG A Q. Study on the application of Ornamental grass in Beijing Chaoyang district park landscape[D]. Hohhot:Inner Mongolia Agricultural University，2022:1-9 杨安琪. 观赏草在北京朝阳区公园景观中的应用研究[D]. 呼和浩特:内蒙古农业大学，2022:1-9
[5] CHEN C，YUAN X H，YANG X J，et al. Research summary and landscape application of ornamental grasses[J]. Chinese Agricultural Science Bulletin，2015，31（19）:135-143 陈超，袁小环，杨学军，等. 观赏草的研究概况和园林应用[J]. 中国农学通报，2015，31（19）:135-143
[6] TIAN H，LIU Y，ZHANG H S，et al. Studies on drought resistance of 6 Pennisetum alopecuroides （L.） Spreng at seed germination stage under PEG-6000 stress[J]. Hubei Agricultural Sciences，2013，52（24）:6115-6119 田宏，刘洋，张鹤山，等. PEG-6000胁迫下6份狼尾草萌发期抗旱性研究[J]. 湖北农业科学，2013，52（24）:6115-6119
[7] WU J Y. Pennisetum alopecuroides with excellent ecological and ornamental effects[J]. China Flowers Horticulture，2003（21）:32 武菊英. 生态和观赏效果俱佳的狼尾草[J]. 中国花卉园艺，2003（21）:32
[8] NIU C M. The aesthetic value of ornamental grasses and their application in landscape design in Zhengzhou city[J]. Contemporary Horticulture，2022，45（12）:102-105 牛长明. 观赏草的美学价值及在郑州市园林景观中的应用[J]. 现代园艺，2022，45（12）:102-105
[9] YANG X J，WU J Y，TENG W J，et al. Study on the photosynthetic characteristics of five Pennisetum speices[J]. Chinese Agricultural Science Bulletin，2013，29（1）:122-126 杨学军，武菊英，滕文军，等. 5种狼尾草属观赏草光合特性研究[J]. 中国农学通报，2013，29（1）:122-126
[10] HU Y F，FAN X F，TENG K，et al. Effects of cutting position， time and IBA concentrate on shoot cuttings propagation survival of Chinese fountaingrass （Pennisetum alopecuroides ‘Ziguang’）[J]. Acta Agrestia Sinica，2018，26（4）:928-934 胡耀芳，范希峰，滕珂，等. 扦插部位、时间和IBA浓度对‘紫光’狼尾草（Pennisetum alopecuroides ‘Ziguang’）茎秆扦插成活的影响[J]. 草地学报，2018，26（4）:928-934
[11] JIANG S L，LI Z B，XU L J，et al. Research progress on polyploid breeding of ornamental plants[J]. Journal of Hebei Forestry Science and Technology，2015（6）:82-85 蒋淑磊，李志斌，徐立军，等. 观赏植物多倍体育种研究进展[J]. 河北林业科技，2015（6）:82-85
[12] YAN C G. Polyploidy and applications of plants[J]. Bulletin of Biology，2007（4）:14-18 晏春耕. 植物多倍体及其应用[J]. 生物学通报，2007（4）:14-18
[13] WANG Q，QIAN R，WEN S Q，et al. Study on chromosome doubling technology of peppermint induced by colchicine[J]. Journal of Hebei Agricultural Sciences，2023，27（4）:83-87，95 王倩，钱荣，温赛群，等. 秋水仙素诱导薄荷染色体加倍技术研究[J]. 河北农业科学，2023，27（4）:83-87，95
[14] SONG Y，ZHANG M，ZHANG C W，et al. Creation of a new material of high yield and disease resistant autotetraploid non-heading Chinese cabbage Huangxinwu[J]. Journal of Nuclear Agricultural Sciences，2022，36（7）:1285-1292 宋莹，张咪，张昌伟，等. 高产、抗病同源四倍体不结球白菜黄心乌新材料的创制[J]. 核农学报，2022，36（7）:1285-1292
[15] ZHANG X Y，ZHANG H L，MU T，et al. Effects of external hormones on induction and differentiation of callus of Chinese Pennisetum[J]. Pratacultural Science，2012，29（7）:1066-1071 张晓莹，张瀚俪，牟彤，等. 外源激素对紫穗狼尾草愈伤组织诱导及分化的影响[J]. 草业科学，2012，29（7）:1066-1071
[16] YOU X T，JING F L，ZHANG M Q，et al. Occurrence and control measures of browning in plant tissue culture[J]. Journal of Mountain Agriculture and Biology，2024，43（2）:34-39 尤小婷，经福林，张曼其，等. 植物组织培养褐化现象的发生及防治措施[J]. 山地农业生物学报，2024，43（2）:34-39
[17] JIA M，DONG S H，CHAO L M，et al. Polyploid induction and identification of Carex breviculmis ‘Siji’[J]. Northern Horticulture，2024（15）:52-59 贾明，董双慧，潮洛濛，等. ‘四季’青绿苔草多倍体诱导及鉴定[J]. 北方园艺，2024（15）:52-59
[18] WU H Y，YUE Y S，WU J Y，et al. Induction and phenotypic analysis of tetraploid Miscanthus sinensis ‘Variegatus’[J]. Molecular Plant Breeding，2020，18（9）:3018-3023 吴海燕，岳跃森，武菊英，等. ‘细叶’芒（Miscanthus sinensis ‘Variegatus’）四倍体诱导与表型分析[J]. 分子植物育种，2020，18（9）:3018-3023
[19] WU H Y. Phenotypic Analysis and physiological study of lowland octoploid Panicum virgatum L.[D]. Hohhot:Inner Mongolia Normal University，2019:1-22 吴海燕. 低地型八倍体柳枝稷（Panicum virgatum L.）的表型分析及生理研究[D]. 呼和浩特:内蒙古师范大学，2019:1-22
[20] MIAO S，FAN J H. Callus induction and plant regeneration from young spike of Pennisetum setaceum[J]. Journal of Anhui Agricultural University，2011，38（2）:255-258 缪珊，范继红. 绒毛狼尾草幼穗的愈伤组织诱导与植株再生[J]. 安徽农业大学学报，2011，38（2）:255-258
[21] DOLEZEL J，GREILHUBER J，SUDA J. Estimation of nuclear DNA content in plants using Flow Cytometry[J]. Nature Protocols，2007，2（9）:2233-2244
[22] LIU Y，ZHAO C R，WANG L F，et al. Wheat root-tip chromosome preparation and plant ploidy identification[J]. Journal of Anhui Agricultural Sciences，2012，40（25）:12349-12350 刘莹，赵翠荣，王立峰，等. 小麦根尖染色体制片及植株倍性鉴定[J]. 安徽农业科学，2012，40（25）:12349-12350
[23] 郑甲成，詹秋文，李杰勤，等. 一种禾本科植物叶片表皮层气孔密度的估测方法[P]. 安徽:CN201710384484.8，2017-09-19
[24] HE J. Study on tetraploid induction of 9501 Paulownia and Platanus orientalis L.[D]. Zhengzhou:Henan Agricultural University，2010:24-34 何佳. 9501泡桐和悬铃木四倍体诱导的研究[D]. 郑州:河南农业大学，2010:24-34
[25] ZHANG S H. Inducement and comparison of cold tolerance in polyploid Betula platyphylla[D]. Harbin:Northeast Forestry University，2023:1-21 张姝慧. 白桦多倍体获得及耐寒性比较[D]. 哈尔滨:东北林业大学，2023:1-21
[26] XIE T W. Research on polyploid induction by colchicine in Zamioculcas Zamiifolia Engl.[D]. Haikou:Hainan University，2012:1-34 谢添伟. 秋水仙素诱导金钱树染色体加倍的技术研究[D]. 海口:海南大学，2012:1-34
[27] BHUSARE B P，JOHN C K，BHATT V P，et al. Colchicine induces tetraploids in in vitro cultures of Digitalis lanata Ehrh.: Enhanced production of biomass and cardiac glycosides[J]. Industrial Crops Products，2021，174:114167
[28] BLAKESLEE A F，AVERY A G. Methods of inducing doubling of chromosomes in plants[J]. Journal of Heredity， 1937，28（12）:393-411
[29] YUE Y，ZHU Y，FAN X，et al. Generation of octoploid Switchgrass in three cultivars by colchicine treatment[J]. Industrial Crops Products，2017，107:20-21
[30] SHI Q H，LIU P，LIU M J， et al. In vivo fast induction of homogeneous autopolyploids via callus in sour jujube （Ziziphus acidojujuba Cheng et Liu）[J]. Horticultural Plant Journal，2016，2（3）:147-153
[31] LIN J，ZHANG B，ZOU J，et al. Induction of tetraploids in paper mulberry （Broussonetia papyrifera （L.） L’Hér. ex Vent.） by colchicine[J]. BMC Plant Biology.2023，23（1）:574
[32] TANG Z，CHEN D，SONG Z，et al. In vitro induction and identification of tetraploid plants of Paulownia tomentosa[J]. Plant Cell，Tissue and Organ Culture （PCTOC），2010，102（2）:213-220
[33] YANG J H，YANG X H. Research on Mulberry radiation breeding III.Induction and identification of polyploid mutants[J]. Acta Sericologica Sinica，1984（1）:9-12 杨今后，杨新华. 桑辐射育种的研究Ⅲ.多倍突变体的诱导及其鉴定[J]. 蚕业科学，1984（1）:9-12
[34] CHEN J，LI D K，LI T B. Methods for inducing grape polyploids and ploidy identification[J]. Chinese Agricultural Science Bulletin，1994（3）:5-8，41 陈俊，李登科，李太保. 葡萄多倍体诱导方法及倍性鉴定[J]. 中国农学通报，1994（3）:5-8，41
[35] FREDERICK G，GMITTER J，XU B L，et al. Colchicine induced polyploidy in Citrus embryogenic cultures，somatic embryos，and regenerated Plantlets[J]. Plant Science，1991，74（1）:135-141
[36] WEI W，XUE S B. Internal reference standards for DNA determination by Flow Cytometry （literature review）[J]. Military Medical Sciences，1988（4）:260-264 魏文，薛绍白. 流式细胞术测定DNA的内参考标准（文献综述）[J]. 军事医学科学院院刊，1988（4）:260-264
[37] KRUTHIKA H S，RUKMANGADA M S，NAIK V G. Genome size，chromosome number variation and its correlation with stomatal characters for assessment of ploidy levels in a core subset of mulberry（Morus spp.） germplasm[J]. Gene，2023，881:147637
[38] ENG W S，HO W S. Polyploidization using colchicine in horticultural plants:A review[J]. Scientia Horticulturae，2019，246:604-617
[39] MO L，CHEN J H，CHEN F，et al. Induction and characterization of polyploids from seeds of Rhododendron fortunei L.[J]. Journal of Integrative Agriculture，2020，19（8）:2016-2026
[40] ZHANG H H. Construction and evaluation of polyploid induction system for Betula pendula ‘Dalecarlica’[D]. Harbin:Northeast Forestry University，2024:1-17 张行行. 裂叶桦多倍体诱导体系构建及评价[D]. 哈尔滨:东北林业大学，2024:1-17
[41] BHATTARAI K，KAREEM A， DENG Z. In vivo Induction and characterization of polyploids in Gerbera Daisy[J]. Scientia Horticulturae，2021:282
[42] WU T，JIA R D，YANG S H，et al. Research advances and prospects on Phalaenopsis polyploid breeding[J]. Acta Horticulturae Sinica，2022，49（2）:448-462 吴婷，贾瑞冬，杨树华，等. 蝴蝶兰多倍体育种研究进展与展望[J]. 园艺学报，2022，49（2）:448-462
[43] HUY N P，TAM D T T，LUAN V Q，et al. In vitro polyploid induction of Paphiopedilum Villosum using colchicine[J]. Scientia Horticulturae，2019，252:283-290

四倍体‘紫光’狼尾草的诱导及鉴定分析

Induction and Identification Analysis of Tetraploid Pennisetum alopecuroides ‘Ziguang’

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	王晶, 伏兵哲. 基于文献计量分析的禾本科牧草染色体加倍研究进展[J]. 草地学报, 2021, 29(3): 413-424.
[2]	杨宇晨, 陈智勇, 夏亚男, 蒋建雄, 易自力. 不同诱变处理对芒多倍体诱导效果的研究[J]. 草地学报, 2014, 22(5): 1069-1074.