外源一氧化氮对遮阴胁迫下高羊茅抗氧化系统的调控机理

doi:10.11733/j.issn.1007-0435.2014.01.017

›› 2014, Vol. 22 ›› Issue (1): 107-115.DOI: 10.11733/j.issn.1007-0435.2014.01.017

外源一氧化氮对遮阴胁迫下高羊茅抗氧化系统的调控机理

付娟娟, 孙永芳, 褚希彤, 许岳飞, 呼天明

西北农林科技大学动物科技学院草业科学系, 陕西杨凌 712100

收稿日期:2013-05-13 修回日期:2013-06-24 出版日期:2014-02-15 发布日期:2014-01-28
通讯作者: 许岳飞，E-mail：xuyfgrass@gmail.com;呼天明，E-mail：hutianming@126.com
作者简介:付娟娟（1987-），女，蒙古族，内蒙古赤峰人，硕士研究生，研究方向为植物逆境生理生态，E-mail：fujuanjuan1234@126.com
基金资助:
西北农林科技大学基本科研业务费专项基金项目（QN2011100）；国家科技支撑计划项目（2011BAD17B05）资助

Physiological Regulation of Nitric Oxide (NO) to the Antioxidant System of Tall Fescue Leaves under Shade Stress

FU Juan-juan, SUN Yong-fang, CHU Xi-tong, XU Yue-fei, HU Tian-ming

Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2013-05-13 Revised:2013-06-24 Online:2014-02-15 Published:2014-01-28

摘要/Abstract

摘要： 在不同遮阴胁迫下（20%遮阴、60%遮阴和90%遮阴），采用叶面喷施外源一氧化氮（NO）方法，研究了外源NO对遮阴胁迫下高羊茅瑞德3号（Festuca arundinacea ‘Arid 3’）的形态指标、叶绿素含量、膜透性、丙二醛（MDA）含量、抗氧化酶活性及脯氨酸含量的影响。结果表明：20%遮阴对高羊茅植株几乎没有伤害，而60%和90%遮阴的伤害作用较严重，并且随着遮阴时间的增加高羊茅受伤害程度加强。外源NO可以显著提高遮阴胁迫下高羊茅叶片的叶绿素含量，降低质膜相对透性的增加，减少膜质过氧化产物MDA含量的升高，促进脯氨酸的积累，提高超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性。外源NO的保护作用随着遮阴时间与遮阴强度的增加而变化，外源NO对20%遮阴和60%遮阴的高羊茅效果较好，而对90%遮阴高羊茅效果较差；此外，随着遮阴时间增加，外源NO对不同遮阴强度高羊茅作用效果不同，处理14 d效果较好。综上表明，外源NO对遮阴胁迫下的高羊茅具有一定的保护作用，但是这种保护作用随着遮阴时间与遮阴强度的增加而下降。

关键词: 高羊茅, 遮阴胁迫, 一氧化氮, 抗氧化系统, 调控机理

Abstract: The effect of NO on oxidative damage caused by shade was investigated in tall fescue (Festuca arundinacea‘Arid 3’) leaves. Tall fescue seedlings sprayed with NO donor (sodium nitroprusside, SNP) were subjected to 0%, 20%, 60%, 90% shade stress. Treatment of tall fescue leaves with 0.1 mM SNP before shade stress alleviated shade-induced membrane permeability, malondialdehyde contents. However, the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), as well as chlorophyll (Chl) content and proline accumulation in tall fescue leaves increased in the presence of SNP under shade stress. Moreover, the protective effect of NO varied according to shade time and intensity. Exogenous NO showed better protective effects under 20% and 60% shade, but a less influence under 90% shade. On the other hand, there was a significant difference between different exposure times. Treatments with SNP under 20%, 60% and 90% shade for two weeks showed the best effect. It could be concluded that NO might act as a signaling molecule to enhance antioxidant enzyme activities, thereby protecting tall fescue leaves from oxidative injures under shade stress. However, the protective effect of NO declined with the increases of treatment time and intensity.

Key words: Tall fescue, Shade stress, Nitric oxide, Antioxidant enzyme, Regulation mechanisim

中图分类号:

付娟娟, 孙永芳, 褚希彤, 许岳飞, 呼天明. 外源一氧化氮对遮阴胁迫下高羊茅抗氧化系统的调控机理[J]. , 2014, 22(1): 107-115.

FU Juan-juan, SUN Yong-fang, CHU Xi-tong, XU Yue-fei, HU Tian-ming. Physiological Regulation of Nitric Oxide (NO) to the Antioxidant System of Tall Fescue Leaves under Shade Stress[J]. , 2014, 22(1): 107-115.

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参考文献

[1] 马鲁沂, 孙小玲, 郝俊, 等.遮阴对高羊茅和沿阶草生活力及抗氧化酶体系的影响[J].草地学报, 2009, 17(2):187-192
[2] Jiang Y W, Duncan R R, Carrow R N. Assessment of low light tolerance of seashore paspalum and bermudagrass[J]. Crop Science, 2004, 44(2):587-594
[3] 许岳飞, 金晶炜, 陈浩, 等.草坪植物耐弱光机理研究进展[J].草地学报, 2011, 19(6):1064-1069
[4] Beligni M V, Lamattina L. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants[J]. Planta, 2000, 210(2):215-221
[5] Xu J, Yin H X, Liu X J, et al. Nitric oxide alleviates Fe deficiency-induced stress in Solanum nigrum [J]. Biologia Plantarum, 2009, 53(4):784-788
[6] Xue L G, Li S W, Sheng H M, et al. Nitric oxide alleviates oxidative damage induced by enhanced ultraviolet-B radiation in cyanobacterium[J]. Current Microbiology, 2007, 55(4):294-301
[7] Song L L, Ding W, Shen J, et al. Nitric oxide mediates abscisic acid induced thermotolerance in the calluses from two ecotypes of reed under heat stress[J]. Plant Science, 2008, 175(6):826-832
[8] Arasimowicz M, Floryszak-Wieczorek J. Nitric oxide as a bioactive signalling molecule in plant stress responses[J]. Plant Science, 2007, 172(5):876-887
[9] 许岳飞, 金晶炜, 孙小玲, 等. 一氧化氮对强光胁迫下高羊茅叶片抗氧化系统的调节效应[J]. 生态学杂志, 2009, 28(12):2471-2476
[10] 熊雪, 周禾, 高伟平.遮阴胁迫下外源一氧化氮对高羊茅的生理调控作用[J]. 草地学报, 2012, 20(2):331-335
[11] Song L L, Ding W, Zhao M G, et al. Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed[J]. Plant Science, 2006, 171(4):449-458
[12] 张殿忠, 汪沛洪, 赵会贤.测定小麦叶片游离脯氨酸含量的方法[J].植物生理学通讯, 1990, 26(4):62-65
[13] Beauchamp C, Fridovich I. Superoxide dismutase improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry, 1971, 44(1):276-287
[14] Hammerschmidt R, Nuckles E M, Kuc J. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrchum lagenarium[J]. Physiological and Molecular Plant Pathology, 1982, 20(1):73-82
[15] Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant physiology, 1992, 98(4):1222-1227
[16] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1/2):248-254
[17] Bell G E, Danneberger T K. Temporal shade on creeping bentgrass turf[J]. Crop Science, 1999, 39(4):1142-1146
[18] Koh K J, Bell G E, Martin D L, et al. Shade and airflow restriction effects on creeping bentgrass golf greens[J]. Crop Science, 2003, 43(6):2182-2188
[19] Beligini M V, Lamattina L. Is nitric oxide toxic or protective?[J]. Trends in Plant Science, 1999, 4(8):299-300
[20] Beligini M V, Lamattina L. Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues[J]. Planta, 1999, 208(3):337-344
[21] Logan B A, Demming-Adams B, Adams Ⅲ W W, et al. Antioxidants and xanthophylls cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field[J]. Journal of Experimental Botany, 1998, 49(328):1869-1879
[22] Zhao L, He J X, Wang X M, et al. Nitric oxide protects against polyethylene glycol-induced oxidative damage in two ecoypes of reed suspension cultures[J]. Journal of Plant Physiology, 2008, 165(2):182-191
[23] Wendehenne D, Pugin A, Klessig D, et al. Nitric oxide: Comparative synthesis and signaling in animal and plant cells[J]. Trends in Plant Science, 2001, 6(4):177-183
[24] Kishor P B K, Sangam S, Amrutha R N, et al. Regulation of praline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance[J]. Current Science, 2005, 88(3):424-438
[25] Liu C C, Liu Y G, Gao K, et al. Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China[J]. Environmental and Experimental Botany, 2011, 71(2):174-183
[26] Farooq M, Basra S M A, Wahid A, et al. Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.)[J]. Journal of Agronomy and Crop Science, 2009, 195(4):254-261

外源一氧化氮对遮阴胁迫下高羊茅抗氧化系统的调控机理

Physiological Regulation of Nitric Oxide (NO) to the Antioxidant System of Tall Fescue Leaves under Shade Stress

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