外源褪黑素对盐胁迫下燕麦幼苗生长及抗氧化系统的影响

doi:10.11733/j.issn.1007-0435.2023.02.011

草地学报 ›› 2023, Vol. 31 ›› Issue (2): 396-403.DOI: 10.11733/j.issn.1007-0435.2023.02.011

• 研究论文 • 上一篇

外源褪黑素对盐胁迫下燕麦幼苗生长及抗氧化系统的影响

李嘉文, 麻冬梅, 苏立娜, 张玲, 侯汶君, 杭嘉慧

宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地/西北退化生态系统恢复与重建教育部重点实验室, 宁夏大学生态环境学院, 宁夏银川 750021

收稿日期:2022-09-23 修回日期:2022-11-07 发布日期:2023-02-28
通讯作者: 麻冬梅,E-mail:576494584@qq.com
作者简介:李嘉文(1997-),女,汉族,四川南充人,硕士研究生,主要从事牧草分子育种,E-mail:1176819350@qq.com
基金资助:
国家自然科学基金项目(32160328)；国家自然科学基金项目(31760698)；自治区重点研发项目课题(2019BBF02022)资助

Effects of Exogenous Melatonin on Oat Seedling Growth and Antioxidant System under Salt Stress

LI Jia-wen, MA Dong-mei, SU Li-na, ZHANG Ling, HOU Wen-jun, HANG Jia-hui

Cultivation Base of State Key Laboratory of Land Degradation and Ecosystem Restoration in Northwest China, Key Laboratory of Restoration and Restoration of Degraded Ecosystems in Northwest China, Ministry of Education, College of Eco-Environment, Ningxia University, Yinchuan, Ningxia 750021, China

Received:2022-09-23 Revised:2022-11-07 Published:2023-02-28

摘要/Abstract

摘要： 褪黑素作为一种吲哚胺类生长激素，具有多种生物学功能，能够调节植物的逆境胁迫中的防御反应。为探究外源褪黑素对盐胁迫下耐盐饲用燕麦(Avena sativa L.)幼苗'白燕7号'的表型和生理的调节作用。本研究在人工气候室进行，采用根灌水培培养，比较在单独盐胁迫和盐与褪黑素复合处理下，'白燕7号'表型指标、渗透调节物质、膜脂过氧化程度、抗氧化系统活性、编码抗氧化物酶相关基因的表达量变化。研究结果表明：与盐胁迫处理相比，添加外源褪黑素后'白燕7号'幼苗的株高和根长显著增高，氧化胁迫造成的膜损伤减轻，且脂氧合酶相关基因LOX表达水平下调，同时渗透失衡得到有效缓解；在清除活性氧方面，编码抗氧化物酶的相关基因表达量上调，酶活性显著提高，抗氧化系统活性显著提升，外源褪黑素最为适宜的浓度为150 μmol·L^-1。这些结果说明外源褪黑素在缓解燕麦盐胁迫方面起重要作用。

关键词: 燕麦, 外源褪黑素, 抗氧化系统

Abstract: Melatonin is an indoleamine growth hormone with multiple biological functions,as well as has an ability to regulate defense responses in plants under stress of adversity. The present study was conducted to explore the effects of exogenous melatonin on the phenotype,physiological indicators,enzyme activities and their related genes in oats (Avena sativa L.) seedlings under salt stress. This study was conducted in an artificial climate chamber and cultured by root irrigation to compare the changes in phenotypic indicators,osmotic regulatory substances,degree of membrane lipid peroxidation,antioxidant system activity,and expression levels of genes related to enzymes encoding antioxidants in 'Baiyan7' under the seven treatments of the single salt stress and combined treatment with salt and melatonin.The results showed that compared with salt stress,the plant height and root length of 'Baiyan 7' seedlings were significantly increased after the addition of exogenous melatonin,the membrane damage caused by oxidative stress was reduced,the expression level of lipoxygenase-related genes LOX was down-regulated,while osmotic imbalance was effectively alleviated;in terms of scavenging reactive oxygen species,the expression of related genes encoding antioxidant enzymes was up-regulated,the enzyme activity was significantly increased,and the activity of antioxidant system was significantly enhanced,which was most suitable at the added concentration of 150 μmol ·L^-1.These results suggest that exogenous melatonin has an important role in alleviating salt stress to oats seedlings.

Key words: Oat, Exogenous melatonin, Antioxidant system

中图分类号:

S544.9

李嘉文, 麻冬梅, 苏立娜, 张玲, 侯汶君, 杭嘉慧. 外源褪黑素对盐胁迫下燕麦幼苗生长及抗氧化系统的影响[J]. 草地学报, 2023, 31(2): 396-403.

LI Jia-wen, MA Dong-mei, SU Li-na, ZHANG Ling, HOU Wen-jun, HANG Jia-hui. Effects of Exogenous Melatonin on Oat Seedling Growth and Antioxidant System under Salt Stress[J]. Acta Agrestia Sinica, 2023, 31(2): 396-403.

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外源褪黑素对盐胁迫下燕麦幼苗生长及抗氧化系统的影响

Effects of Exogenous Melatonin on Oat Seedling Growth and Antioxidant System under Salt Stress

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