冷胁迫对2种垂穗披碱草生长和生理特性的影响

doi:10.11733/j.issn.1007-0435.2014.04.017

草地学报 ›› 2014, Vol. 22 ›› Issue (4): 789-795.DOI: 10.11733/j.issn.1007-0435.2014.04.017

冷胁迫对2种垂穗披碱草生长和生理特性的影响

付娟娟¹, 刘建¹, 孙永芳¹, 褚希彤¹, 苗彦军², 呼天明¹, 许岳飞¹

1. 西北农林科技大学动物科技学院, 陕西杨凌 712100;
2. 西藏农牧学院植物科学学院, 西藏林芝 860000

收稿日期:2013-10-21 修回日期:2013-12-16 出版日期:2014-08-15 发布日期:2014-08-04
通讯作者: 呼天明, 许岳飞
作者简介:付娟娟（1987-），女，蒙古族，内蒙古赤峰人，硕士研究生，研究方向为植物逆境生理生态，E-mail：fujuanjuan1234@126.com
基金资助:
西北农林科技大学基本科研业务费专项（QN2011100）；国家科技支撑计划（2011BAD17B05）（2011BAC09B03）资助

Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties

FU Juan-juan¹, LIU Jian¹, SUN Yong-fang¹, CHU Xi-tong¹, MIAO Yan-jun², HU Tian-ming¹, XU Yue-fei¹

1. Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Plant Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China

Received:2013-10-21 Revised:2013-12-16 Online:2014-08-15 Published:2014-08-04

摘要/Abstract

摘要：

低温是影响植物生长的外界限制因子之一。采用沙培法研究了冷胁迫对2种垂穗披碱草（Elymus nutans）（当雄垂穗披碱草（西藏野生）和甘南垂穗披碱草）生长与生理特性的影响。结果表明：冷胁迫处理5d显著抑制2种垂穗披碱草的生长和叶绿素的合成，特别是甘南垂穗披碱草发生严重氧化损伤，电解质渗透率和丙二醛（MDA）含量分别比对照增加114.7%和124.6%，而当雄垂穗披碱草受伤害较轻，分别比对照增加18.2%和10.8%。冷胁迫也显著增加了甘南垂穗披碱草中H₂O₂和自由基的积累，而当雄垂穗披碱草与对照相比差异不显著。冷胁迫处理5d后，当雄垂穗披碱草的类胡萝卜素含量较甘南增加72.3%，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性分别较对照增加80.3%，27.3%和65.9%；甘南垂穗披碱草的SOD和POD活性分别较对照增加11.4%和28.9%，而其CAT活性下降25.5%。相关分析表明，垂穗披碱草在冷胁迫下细胞膜受损伤程度与抗氧化酶活性负相关。当雄垂穗披碱草通过提高抗氧化酶活性，可有效清除体内积累的活性氧，减轻细胞膜系统受损伤的程度从而提高其抗寒性。

关键词: 冷胁迫, 膜脂过氧化, 活性氧, 抗氧化酶, 垂穗披碱草

Abstract:

Low temperature is one of the limiting factors affecting plant growth. The growths and physiological characteristics of Tibetan wildElymus nutans and Gannan E. nutans were studied under cold stress with sand culture in this paper. The results showed that cold stress significantly inhibited the seedling growths and chlorophyll syntheses of two E. nutans varieties. Gannan E. nutans leaves were more liable to suffer oxidative damage than the wild E. nutans leaves. The electrolyte leakages and MDA contents of tested varieties increased by 114.7%, 124.6% and 18.2%, 10.8% comparing to control, respectively. During the period of cold stress, the accumulations of hydrogen peroxide (H₂O₂) and superoxide radical in E. nutans increased compared with the control. Damxung E. nutans maintained significantly higher carotenoids (Car) content and the activities of antioxidant enzyme, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were 80.3%, 27.3% and 65.9% higher than the control. Correlation analyses indicated there were significant correlations between growth and physiology charaterisitics, as well as lipid peroxidation and antioxidant enzyme activities in E. nutans leaves.It can be concluded that Dangxiong specices exhibits a better tolerance to cold stress than Gannan.

Key words: Cold stress, Lipid peroxidation, ROS accumulation, Antioxidant enzyme, Elymus nutans

中图分类号:

付娟娟, 刘建, 孙永芳, 褚希彤, 苗彦军, 呼天明, 许岳飞. 冷胁迫对2种垂穗披碱草生长和生理特性的影响[J]. 草地学报, 2014, 22(4): 789-795.

FU Juan-juan, LIU Jian, SUN Yong-fang, CHU Xi-tong, MIAO Yan-jun, HU Tian-ming, XU Yue-fei. Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties[J]. Acta Agrestia Sinica, 2014, 22(4): 789-795.

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冷胁迫对2种垂穗披碱草生长和生理特性的影响

Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties

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