根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究

doi:10.11733/j.issn.1007-0435.2024.01.021

草地学报 ›› 2024, Vol. 32 ›› Issue (1): 198-206.DOI: 10.11733/j.issn.1007-0435.2024.01.021

根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究

张玲¹, 麻冬梅¹, 刘晓霞¹, 马巧利², 侯汶君¹, 李嘉文¹, 苏立娜¹, 杭嘉慧¹

1. 宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地/西北退化生态系统恢复与重建教育部重点实验室, 宁夏大学生态环境学院, 宁夏银川 750021;
2. 宁夏大学林业与草业学院, 宁夏银川 750021

收稿日期:2023-05-25 修回日期:2023-08-07 出版日期:2024-01-15 发布日期:2024-01-30
通讯作者: 麻冬梅,E-mail:576494584@qq.com
作者简介:张玲(1997-),女,汉族,山东济南人,硕士研究生,主要从事牧草分子育种研究,E-mail:1962457361@qq.com
基金资助:
国家自然科学基金项目（32160328）；自治区重点研发重点项目（2022BBF02030）；自治区重点研发重点项目课题（2022BDE92070）；宁夏牧草育种专项课题（2019NYYZ0401）资助

The Effects of Exogenous Melatonin on Seedling Physiological Characteristics of Alfalfa under Drought Stress

ZHANG Ling¹, MA Dong-mei¹, LIU Xiao-xia¹, MA Qiao-li², HOU Wen-jun¹, LI Jia-wen¹, SU Li-na¹, HANG Jia-hui¹

1. Cultivation Base of State Key Laboratory of Land Degradation and Ecosystem Restoration in Northwest China, Key Laboratory of Restoration and Reconstruction of Degraded Ecosystems in northwestern China of Ministry of Education, College of Eco-Environment, Ningxia University, Yinchuan, Ningxia 750021, China;
2. College of Forestry and Pratacalltture, Ningxia University Yinchuan, Ningxia 750021, China

Received:2023-05-25 Revised:2023-08-07 Online:2024-01-15 Published:2024-01-30

摘要/Abstract

摘要： 为探究褪黑素对干旱胁迫下紫花苜蓿（Medicago sativa L.）生长的影响，本研究以‘中苜3号’紫花苜蓿为试验材料，设置正常供水和干旱2个水分梯度，采用盆栽试验根灌不同浓度褪黑素，分析不同条件下幼苗表型、生理生化指标的变化。结果表明：90 μmol·L^-1褪黑素处理能够显著提高紫花苜蓿幼苗株高、茎粗、相对含水量、相对叶绿素含量，此外，90 μmol·L^-1褪黑素处理显著增加干旱胁迫下紫花苜蓿幼苗叶片抗氧化酶活性及酶合成相关基因表达水平，并降低脯氨酸、可溶性糖含量及丙二醛、相对电导率、过氧化氢含量。综上所述，褪黑素提高干旱胁迫下紫花苜蓿幼苗抗氧化酶生物合成基因表达水平，增强抗氧化酶活性，清除过量活性氧，缓解膜损伤，提高幼苗抗旱性。

关键词: 紫花苜蓿, 干旱, 褪黑素, 渗透调节, 抗氧化酶

Abstract: To investigate the effects of exogenous melatonin (MT) on the growth of alfalfa (Medicago sativa) under drought stress, this study used the cultivar ‘Zhongmu 3’ alfalfa as experimental material. Under drought stress treatments, potted alfalfa seedlings were root-irrigated with different concentrations of melatonin. Phenotypic and physiological-biochemical indicators of seedlings were measured to further explore the optimal concentration of MT for alleviating drought stress. The results showed that treatment with 90 μmol·L^-1 melatonin significantly increased the plant height, stem thickness, relative water content, and relative chlorophyll content of the alfalfa seedlings. In addition, 90 μmol·L^-1 MT treatment significantly increased the antioxidant enzyme activities and the gene expression levels of the enzymes in the leaves of alfalfa seedlings under drought stress. The treatment with 90 μmol·L^-1 MT also reduced the contents of proline, soluble sugar, malondialdehyde, electrolyte leakage, and hydrogen peroxide content. Therefore, root irrigation of 90 μmol·L^-1 MT is helpful to improve drought resistance of alfalfa seedlings. This study provides certain theoretical support for planting efficient forage alfalfa in arid areas.

Key words: Alfalfa, Drought, Melatonin, Osmotic adjustment, Antioxidase

中图分类号:

Q945.78

张玲, 麻冬梅, 刘晓霞, 马巧利, 侯汶君, 李嘉文, 苏立娜, 杭嘉慧. 根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究[J]. 草地学报, 2024, 32(1): 198-206.

ZHANG Ling, MA Dong-mei, LIU Xiao-xia, MA Qiao-li, HOU Wen-jun, LI Jia-wen, SU Li-na, HANG Jia-hui. The Effects of Exogenous Melatonin on Seedling Physiological Characteristics of Alfalfa under Drought Stress[J]. Acta Agrestia Sinica, 2024, 32(1): 198-206.

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根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究

The Effects of Exogenous Melatonin on Seedling Physiological Characteristics of Alfalfa under Drought Stress

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