草地学报

草地学报 ›› 2024, Vol. 32 ›› Issue (1): 322-330.DOI: 10.11733/j.issn.1007-0435.2024.01.033

• 技术研发 • 上一篇    下一篇

萎缩芽孢杆菌WL520生物活性及干旱胁迫下促紫花苜蓿生长效应

武玲玲1, 谢永丽1,2,3, 杨杰1, 杨雪1,2, 李俊熹1, 王添1, 高英1, 常斐斐1   

  1. 1. 青海大学农牧学院, 青海 西宁 810016;
    2. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海 西宁 810016;
    3. 青海省青藏高原优良牧草种质资源利用重点实验室, 青海 西宁 810016
  • 收稿日期:2023-06-12 修回日期:2023-09-12 出版日期:2024-01-15 发布日期:2024-01-30
  • 通讯作者: 谢永丽,E-mail:986237342@qq.com
  • 作者简介:武玲玲(1998-),女,汉族,青海门源人,硕士研究生,主要从事微生物资源的研究,E-mail:2736577439@qq.com
  • 基金资助:
    青海省科技厅应用基础研究项目（2023-ZJ-709）；国家自然科学基金项目（32160030）资助

Biological Activity of Bacillus WL520 and Its Growth-promoting Effect on Alfalfa under Drought Stress

WU Ling-ling1, XIE Yong-li1,2,3, YANG Jie1, YANG Xue1,2, LI Jun-xi1, WANG Tian1, GAO Ying1, CHANG Fei-fei1   

  1. 1. College of Agriculture and Animal Husbandry, Qinghai University 810016, China;
    2. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University 810016, China;
    3. Key Laboratory of Use of Forage Germplasm Resources on Tibetan Plateau of Qinghai Province, Xining, Qinghai Province 810016, China
  • Received:2023-06-12 Revised:2023-09-12 Online:2024-01-15 Published:2024-01-30

PDF

摘要： 为增强紫花苜蓿‘新牧1号’（Medicago sativa L.‘Xinmu 1’）的耐旱性，以分离筛选自青海乌兰干旱沙地白刺（Nitraria tangutorum）根际的萎缩芽孢杆菌（Bacillus atrophaeus）WL520对紫花苜蓿进行灌根处理，并测定菌株生物活性。通过平板对峙、透明圈法测定菌株拮抗、抑菌酶活性；测定菌株耐旱性及产IAA、固氮活性；采用16S rDNA及gyrB基因测序对菌株进行分子鉴定；并测定干旱胁迫下，菌株促紫花苜蓿的生长效应。结果表明：菌株WL520对2种病原真菌表现出拮抗活性，可产生4种抑菌水解酶；并表现出较强耐旱性、产IAA及固氮能力；分子鉴定为萎缩芽孢杆菌（Bacillus atrophaeus）。与干旱胁迫（D）相比，菌液灌根（DB）后，紫花苜蓿株高、根长及鲜重提高，叶绿素及SOD含量提高，MDA含量下降。因此，干旱胁迫下，菌株WL520对紫花苜蓿具有显著促生效应，本研究为挖掘高原微生物资源及促牧草生长提供了优异菌株及理论基础。

关键词: 萎缩芽孢杆菌, 生物活性, 干旱胁迫, 紫花苜蓿

Abstract: In order to enhance the drought tolerance of Medicago sativa L. ‘Xinmu 1’, Bacillus atrophaeus WL520 was isolated from the rhizosphere of Nitraria tangutorum grown in the arid sandy land of Wulan, Qinghai Province, and then irrigated Alfalfa. The biological activity of the strain was determined. The antagonistic activity, antibacterial enzyme activity, the drought tolerance, IAA production and nitrogen fixation activity of the strain were measured. Molecular identification of the strain was conducted by 16S rDNA and gyrB gene sequencing. The growth-promoting effect of the strain on Medicago sativa under drought stress was determined. The results showed that the strain WL520 exhibited antagonistic activity against pathogens, produced four antibacterial hydrolytic enzymes, showed strong drought tolerance, IAA production and nitrogen fixation ability, and was identified as Bacillus atrophaeus. Compared with drought stress without the strain, after root irrigation with the bacterial suspension of strain WL520, the plant height, root length and fresh weight of Medicago sativa increased, the chlorophyll and SOD content increased, and the MDA content decreased, indicating that strain WL520 had a significant growth-promoting effect on Medicago sativa under drought stress. This study provided excellent strains and theoretical basis for the exploration of highland microbial resources and growth promoting of pasture.

Key words: Bacillus atrophaeus, Biological activity, Drought stress, Alfalfa

中图分类号: