硒引发对紫花苜蓿幼苗抗氧化特性的影响

doi:10.11733/j.issn.1007-0435.2023.09.033

草地学报 ›› 2023, Vol. 31 ›› Issue (9): 2875-2881.DOI: 10.11733/j.issn.1007-0435.2023.09.033

硒引发对紫花苜蓿幼苗抗氧化特性的影响

夏方山, 王勃, 阴禹舟, 李尹琳, 岑慧芳, 朱慧森

山西农业大学草业学院, 山西太谷 030801

收稿日期:2023-03-01 修回日期:2023-04-03 出版日期:2023-09-15 发布日期:2023-10-07
通讯作者: 夏方山,E-mail:dqlxfs8583@163.com
作者简介:夏方山(1983-),男,汉族,山东潍坊人,副教授,博士,主要从事草类植物育种与种子科学研究,E-mail:dqlxfs8583@163.com;王勃(1998-),女,汉族,山西晋城人,硕士研究生,主要从事草种子科学研究,E-mail:wangbo10898@163.com
基金资助:
山西农业大学“十四五”生物育种工程项目(YZGC134)；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)；山西省高等学校科技创新项目(2021L106)；山西省基础研究计划青年科学基金项目(20210302124607)；晋中市科技重点研发计划(Y202012)资助

Priming Influence of Selenium on the Antioxidant Characteristics of Alfalfa Seedlings

XIA Fang-shan, WANG Bo, YIN Yu-zhou, LI Yin-lin, CEN Hui-fang, ZHU Hui-sen

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2023-03-01 Revised:2023-04-03 Online:2023-09-15 Published:2023-10-07

摘要/Abstract

摘要： 为探讨外源硒引发对紫花苜蓿(Medicago sativa)幼苗抗氧化能力的影响,采用不同浓度(0,0.5,1.0,2.0,4.0和8.0 mmol·L^-1)的亚硒酸钠溶液在20℃黑暗条件下引发紫花苜蓿种子0(CK),3,6,9和12 h后进行标准发芽试验,并分析其幼苗超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)、谷胱甘肽还原酶(Glutathione reductase,GR)活性及丙二醛(Maloddialdehyde,MDA)含量的变化。结果表明:外源硒浓度和引发时间与紫花苜蓿幼苗的抗氧化能力具有密切关系;低浓度(≤ 1.0 mmol·L^-1)硒引发提高了其幼苗SOD,CAT,APX和GR的活性,均显著高于CK(P<0.05),0.5 mmol·L^-1硒引发时显著降低了其MDA含量(P<0.05);高浓度(≥ 4.0 mmol·L^-1)硒引发12 h时,其幼苗SOD,CAT,APX和GR活性均显著低于CK(P<0.05),其幼苗MDA含量则显著高于CK(P<0.05),说明外源硒引发对紫花苜蓿幼苗抗氧化能力的影响具有双重效应。硒浓度为0.5 mmol·L^-1引发12 h时,紫花苜蓿幼苗的抗氧化酶活性最高,MDA含量最低,这可能是进行紫花苜蓿富硒生产的最佳引发处理。

关键词: 紫花苜蓿, 硒, 种子引发, 幼苗, 抗氧化性能

Abstract: In order to investigate the priming effect of exogenous selenium on the antioxidant capacity of alfalfa (Medicago sativa) seedlings,standard germination tests were conducted on alfalfa seeds after primed with different concentrations (0,0.5,1.0,2.0,4.0 and 8.0 mmol·L^-1) of sodium selenite solution for 0(CK),3,6,9 and 12 h,and changes of the activities of superoxide dismutase (SOD),catalase (CAT),ascorbate peroxidase (APX),glutathione reductase (GR) and malondialdehyde (MDA) content in those seedlings were examined. The results showed that the priming effects of exogenous selenium on the antioxidant capacity of alfalfa seedlings were closely related to its concentration and priming time. Primed with a low concentration (≤ 1.0 mmol·L^-1) of selenium improved the activities of SOD,CAT,APX and GR in the seedlings,which was significantly higher than CK (P<0.05);and priming with 0.5 mmol·L^-1 exogenous selenium significantly reduced the MDA content of alfalfa seedlings. However,the activities of SOD,CAT,APX and GR were significantly lower than CK in alfalfa seedlings undergoing high concentration (≥ 4.0 mmol·L^-1) of selenium primed for 12 h (P<0.05),and their MDA content was significantly higher than CK (P<0.05). These results demonstrated that priming with exogenous selenium had a double effect on the antioxidant capacity of alfalfa seedlings. Primed with 0.5 mmol·L^-1 of exogenous selenium for 12 h,the activities of antioxidant enzymes were at the highest level in alfalfa seedlings and their MDA contents at the lowest level,this might be the best priming treatments for selenium-rich production of alfalfa.

Key words: Alfalfa, Selenium, Seed priming, Seedlings, Oxidation resistance

中图分类号:

S512.6

夏方山, 王勃, 阴禹舟, 李尹琳, 岑慧芳, 朱慧森. 硒引发对紫花苜蓿幼苗抗氧化特性的影响[J]. 草地学报, 2023, 31(9): 2875-2881.

XIA Fang-shan, WANG Bo, YIN Yu-zhou, LI Yin-lin, CEN Hui-fang, ZHU Hui-sen. Priming Influence of Selenium on the Antioxidant Characteristics of Alfalfa Seedlings[J]. Acta Agrestia Sinica, 2023, 31(9): 2875-2881.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2023.09.033

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I9/2875

参考文献

[1] WANG M,PENG Q,ZHOU F,et al. Uptake kinetics and interaction of selenium species in tomato (Solanum lycopersicum L.) seedlings[J]. Environmental Science and Pollution Research,2019,26(10):9730-9738
[2] YANG W J,HUANG G L,CHEN F,et al. Extraction/synthesis and biological activities of selenopolysaccharide[J]. Trends in Food Science and Technology,2021(109):211-218
[3] YIN H,QI Z,LI M,et al. Selenium forms and methods of application differentially modulate plant growth,photosynthesis,stress tolerance,selenium content and speciation in Oryza sativa L[J]. Ecotoxicology and Environmental Safety,2019(169):911-917
[4] DU B,LUO H,HE L,et al. Rice seed priming with sodium selenate:Effects on germination,seedling growth and biochemical attributes[J]. Scientific Reports,2019,9(1):4311
[5] 侯青光,韦林汕,卢亚妮,等. 硒肥不同喷施时期和种类对玉米产量、品质及硒和重金属含量的影响[J]. 西南农业学报,2021,34(9):1900-1907
[6] 贾亚琴,董飞,杨峰,等. 喷施硒肥对黑小麦籽粒产量及硒含量的影响[J]. 麦类作物学报,2021,41(10):1266-1271
[7] LIU T,LIU X T,ZHOU R R,et al. De novo transcriptome assembly and comparative analysis highlight the primary mechanism regulating the response to selenium stimuli in oats (Avena sativa L.)[J]. Frontiers in Plant Science,2021(12):625520
[8] WANG M,ALI F,WANG M K,et al. Understanding boosting selenium accumulation in wheat (Triticum aestivum L.) following foliar selenium application at different stages,forms,and doses.[J]. Environmental Science and Pollution Research,2020,27(1):717-728
[9] 张祎雯,董畅茹,刘小强,等. 硒对水稻吸收转运镉、砷和汞影响机制的研究进展[J]. 生物学杂志,2021,38(5):101-104,109
[10] ADHIKARY S,BISWAS B,CHAKRABORTY D,et al. Seed priming with selenium and zinc nanoparticles modifies germination,growth,and yield of direct-seeded rice (Oryza sativa L.)[J]. Scientific reports,2022(12):7103
[11] WANG M,WANG Y X,GE C H,et al. Foliar selenium nanoparticles application promotes the growth of maize (Zea mays L.) seedlings by regulating carbon,nitrogen and oxidative stress metabolism[J]. Scientia Horticulturae,2023(311):111816
[12] HAO S N,LIU P F,QIN J,et al. Effects of applying different doses of selenite to soil and foliar at different growth stage on selenium content and yield of different oat varieties[J]. Plants,2022,11(14):1810
[13] 施和平,张晓元,许晟. 水培条件下硒对花生幼苗生长和开花及抗氧化酶活性的影响[J]. 热带作物学报,2022,43(12):2489-2495
[14] 王华,张雅娟,周武先,等. 外源硒对白术种子萌发及幼苗生理特性的影响[J]. 分子植物育种,2019,17(22):7551-7558
[15] 郭坤元,吴育中,杨春惠,等. 外源硒对黄精种子萌发及幼苗生理特性的影响[J]. 分子植物育种,2020,18(22):7568-7576
[16] 宁娜,张惠敏,安姝嫔,等. 外源硒对葡萄叶片活性氧代谢及果实贮藏品质的影响[J]. 北方园艺,2022(15):89-97
[17] 成凯,何茂,黄志,等. 硒、硫互作对青花菜幼苗渗透调节物质和抗氧化酶的影响[J]. 西北农业学报,2018,27(1):98-107
[18] 张士敏,朱慧森,赵娇阳,等. 不同硒源及浓度对偏关苜蓿种子萌发及物质转化的影响[J]. 草地学报,2022,30(1):100-107
[19] ETTEIEB S,MAGDOULI S,ZOLFAGHARI M,et al. Monitoring and analysis of selenium as an emerging contaminant in mining industry:A critical review[J]. Science of the Total Environment,2020(698):134339
[20] 王勃,王聪聪,夏方山,等. 硒引发对不同品种紫花苜蓿种子抗氧化特性的影响[J]. 草地学报,2022,30(8):2037-2044
[21] ARUN M N,HEBBAR S S,BHANUPRAKAS K,et al. Seed priming improves irrigation water use efficiency,yield and yield components of summer cowpea under limited water conditions[J]. Legume Research,2017,40(5):864-871
[22] RAJORA N,VATS S,RATURI G,et al. Seed priming with melatonin:A promising approach to combat abiotic stress in plants[J]. Plant Stress,2022(4):100071
[23] MOULICK D,SANTRA S C,GHOSH D. Effect of selenium induced seed priming on arsenic accumulation in rice plant and subsequent transmission in human food chain[J]. Ecotoxicology and Environmental Safety,2018(152):67-77
[24] 韩颜隆,刘晓静,王静,等. 施氮对不同品种紫花苜蓿根系特性和生产性能的影响[J]. 草地学报,2022,30(2):379-384
[25] HORVAT D,VILJEVAC V M,ANDRIC' L,et al. Characterization of forage quality,phenolic profiles,and antioxidant activity in alfalfa (Medicago sativa L.)[J]. Plants,2022,11(20):2735
[26] 昝看卓,刘丛,张燕燕,等. 关中地区紫花苜蓿,小麦及玉米根际土壤理化性质研究[J]. 草地学报,2022,30(5):1246-1252
[27] 郭孝,胡华锋,介晓磊,等. 日粮中添加富硒钴苜蓿草粉对家兔饲料转化率和肉品质的影响[J]. 家畜生态学报,2017,38(12):29-33
[28] 胡华锋,王彦华,李明,等. 叶面施肥对紫花苜蓿产草量及吸硒特性的影响[J]. 草地学报,2015,23(6):1347-1350
[29] 田春丽,李斌,刘芳,等. 硒、锌元素配施对紫花苜蓿产量、植株体内硒锌积累和氨基酸含量的影响[J].草业学报,2019,28(3):142-153
[30] 胡华锋,刘太宇,郭孝,等. 基施硒肥对不同生育期紫花苜蓿吸收、转化及利用硒的影响[J]. 草地学报,2015,23(1):101-106
[31] 夏方山,王聪聪,李红玉,等. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报,2021,29(3):472-477
[32] ALLEN E,ALVAREZ S,DAMMEN S,et al. International rules for seed testing:The germination test[M]. Zurich:International Seed Testing Association,2019:25
[33] KIBINZA S,VINEL D,CÔME D,et al. Sunflower seed deterioration as related to moisture content during ageing,energy metaboli sm and active oxygen species scavenging[J]. Physiologia Plantarum,2006,128(3):496-506
[34] RAO K V M,SRESTY T V S. Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses[J]. Plant Science,2000(157):113-128
[35] AEBI H E. Catalase. in Bergmeyer H U (Ed.),Methods of Enzymatic Analysis[M]. Weinheim:Verlag Chemie,1986:273-286
[36] NAKANO Y,ASADA K. Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplast[J]. Plant and Cell Physiology,1981(22):867-880
[37] MADAMANCHI N R,ALSCHER R G. Metabolic bases for differences in sensitivity of two pea cultivars to sulfur dioxide[J]. Plant Physiology,1991,97(1):88-93
[38] BAILLY C,BENAMAR A,CORBINEAU F,et al. Changes in malondialdehyde content and superoxide dismutase,catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging[J]. Physiologia Plantarum,1996(97):104-111
[39] WOCH W,HAWRYLAK-NOWARK B. Selected antioxidant properties of alfalfa,radish,and white mustard sprouts biofortified with selenium[J]. Acta Agrobotanica,2019,72(2):1768
[40] 于章龙,赵智勇,刘瑞,等. 运黑14207硒赋存形态及抗氧化特性研究[J]. 麦类作物学报,2022,42(3):1-7
[41] XIA F S,WANG M Y,CHEN L L,et al. Responses of mitochondrial ultrastructure and physiological variations to PEG-priming on ultra-dried oat (Avena sativa L.) seeds after ageing[J]. Seed Science and Technology,2017,45(3):622-637
[42] BAI B Q,WANG Z,GAO L M,et al. Effects of selenite on the growth of alfalfa (Medicago sativa L. cv. Sadie 7) and related physiological mechanisms[J]. Acta Physiologiae Plantarum,2019(41):78
[43] 代惠萍,崔科飞,贾根良,等. 紫花苜蓿叶片对硒胁迫的生理响应研究[J]. 北方园艺,2016(17):64-67
[44] PILON-SMITS E A H. On the ecology of selenium accumulation in plants[J]. Plants,2019,8(7):1-17

硒引发对紫花苜蓿幼苗抗氧化特性的影响

Priming Influence of Selenium on the Antioxidant Characteristics of Alfalfa Seedlings

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍. 紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2608-2620.
[2]	何林鑫, 黄荣, 田望军, 靳振海, 赵亮, 陈海雁, 张振粉. 欧文氏菌DE2对三种豆科牧草幼苗生长的影响[J]. 草地学报, 2023, 31(9): 2642-2652.
[3]	李乐, 李丹宁, 杨叶研, 潘新雅, 万懿琦, 张国云, 席杰军, 王亚芳, 杨培志. 干旱胁迫下紫花苜蓿叶片代谢组分析及气孔调节物质的筛选[J]. 草地学报, 2023, 31(9): 2671-2683.
[4]	李红玉, 王聪聪, 夏方山, 杨轩, 李尹琳, 张杰敏. 抗坏血酸引发对燕麦老化种子胚细胞抗氧化性能的影响[J]. 草地学报, 2023, 31(8): 2392-2398.
[5]	阚海明, 徐恒康, 鲁佳男, 孙鑫博, 武菊英. 不同丛枝菌根真菌接种对3种草地植物生长特性的影响[J]. 草地学报, 2023, 31(7): 1922-1930.
[6]	任忠敏, 刘畅, 李珊珊, 李波. 外源激素和⁶⁰Co-γ辐射对苜蓿愈伤组织分化及内源激素水平的影响[J]. 草地学报, 2023, 31(7): 2162-2169.
[7]	岑慧芳, 黄洁琼, 申王晖, 朱慧森, 夏方山, 许涛. 紫花苜蓿MsUGT87A1基因克隆及其对非生物胁迫的响应分析[J]. 草地学报, 2023, 31(6): 1682-1692.
[8]	郁伟杰, 苗一凡, 穆海婷, 贾雪, 闫冬, 徐博. 转录组学解析紫花苜蓿雄性不育系花粉败育机制[J]. 草地学报, 2023, 31(6): 1702-1713.
[9]	于思敏, 罗永忠, 康芳明, 邱应德, 李转桃. 干旱胁迫对紫花苜蓿生长和叶绿素荧光特性的影响[J]. 草地学报, 2023, 31(6): 1762-1771.
[10]	朱琨, 方志坚, 李帅, 王巍, 李波. 冻融及盐碱双重胁迫对苜蓿幼苗营养器官矿质离子吸收及运输的影响[J]. 草地学报, 2023, 31(6): 1818-1825.
[11]	闫星全, 贾玉山, 格根图, 王志军, 赵牧其尔, 刘静怡, 珠娜, 孙鹏波. 原料含水量及添加剂种类对青贮苜蓿品质的影响[J]. 草地学报, 2023, 31(6): 1861-1866.
[12]	王欣怡, 王晓倩, 刘亚玲, 晁跃辉. 紫花苜蓿MsMYB33基因克隆、亚细胞定位及转录自激活检测[J]. 草地学报, 2023, 31(5): 1331-1337.
[13]	陈超超, 李贺洋, 闫雨欣, 宋晴, 黄悠, 热孜亚·艾力, 张铁军. 20份紫花苜蓿种质对黄萎病的抗性评价[J]. 草地学报, 2023, 31(5): 1425-1434.
[14]	王勃, 夏方山, 董秋丽, 岑慧芳, 李尹琳, 张杰敏. 外源油菜素内酯引发对劣变燕麦种子抗氧化特性的影响[J]. 草地学报, 2023, 31(5): 1461-1468.
[15]	路文杰, 吴聪, 齐晋云, 景亚泓. 生物炭和接种剂配施对紫花苜蓿生物量和土壤特征的影响[J]. 草地学报, 2023, 31(5): 1578-1587.