假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析

doi:10.11733/j.issn.1007-0435.2023.09.007

草地学报 ›› 2023, Vol. 31 ›› Issue (9): 2628-2641.DOI: 10.11733/j.issn.1007-0435.2023.09.007

假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析

蒋宇佳^1,2, 于元平^1,2, 孙向一^1,2, 周敏^1,2, 吴春妍^1,2, 李玉华^1,2, 刘明稀^1,2

1. 湖南农业大学农学院草业科学系, 湖南长沙 410128;
2. 湖南省草地研究中心, 湖南长沙 410128

收稿日期:2023-03-20 修回日期:2023-04-27 出版日期:2023-09-15 发布日期:2023-10-07
通讯作者: 刘明稀,E-mail:Lmx75@hunau.edu.cn
作者简介:蒋宇佳(1996-),女,汉族,湖南宁乡人,硕士研究生,主要从事草坪草分子生物学研究,E-mail:1754071305@qq.com
基金资助:
湖南省自然科学基金项目面上项目(2018 JJ2180)；湖南农业大学教改项目(XJJG-2020-033)资助

Identification of R2R3-MYB Gene Family and Analysis of its Expression Pattern in Centipedegrass under Drought Stress

JIANG Yu-Jia^1,2, YU Yuan-Ping^1,2, SUN Xiang-Yi^1,2, ZHOU Min^1,2, WU Chun-Yan^1,2, LI Yu-Hua^1,2, LIU Ming-Xi^1,2

1. Department of Pratacultural Science, College of Agronomy, Hunan Agricultural University, Changsha, Hunan Province 410128, China;
2. Hunan Grassland Research Center, Changsha, Hunan Province 410128, China

Received:2023-03-20 Revised:2023-04-27 Online:2023-09-15 Published:2023-10-07

摘要/Abstract

摘要： R2R3-MYB家族是植物中最大的转录因子家族之一,广泛参与各种生理响应和分子调控。本研究利用假俭草(Eremochloa ophiuroides (Munro.))基因组数据和生物信息学手段鉴定了R2R3-MYB转录因子,并对系统进化、结构、顺式作用元件等进行分析;同时对R2R3-MYB转录因子响应干旱胁迫的表达模式进行分析。结果显示,假俭草中有113个R2R3-MYB成员;蛋白二级结构和三级结构都表明R2R3-MYBs蛋白都含有螺旋-转角-螺旋结构;顺式作用元件分析发现启动子区含有大量植物激素响应和胁迫响应元件,为R2R3-MYB基因进化分析提供了依据。基因表达模式分析表明,与野生型相比,抗旱突变体叶中有39个基因、根中有58个基因在一个或多个干旱时期表达量提高,推测这些R2R3-MYB基因在响应干旱胁迫过程中发挥着重要作用。本研究结果为进一步深入研究假俭草R2R3-MYB基因家族的功能奠定了基础。

关键词: 假俭草, R2R3-MYB基因家族, 生物信息学, 干旱胁迫, 表达模式

Abstract: The R2R3-MYB family,one of the largest transcription factor families in plants,is involved in a wide range of physiological responses and molecular regulation. In this study,R2R3-MYB transcription factors were identified using Eremochloa ophiuroides (Munro.) Genomic data and bioinformatics methods,and the phylogeny,structure and cis-regulatory element were analyzed. At the same time,the expression patterns of R2R3-MYB transcription factor in response to drought stress were analyzed. The results showed that there were 113 R2R3-MYB members in centipedegrass,and the secondary and tertiary structures of the proteins indicated that both R2R3-MYBs proteins contained Helix-turn-helix structure. The cis-regulatory element analysis showed that the promoter region contained a large number of plant hormone response and stress response elements,which provided a basis for the evolutionary analysis of R2R3-MYB gene. Gene expression pattern analysis showed that compared with the wild type,39 genes in leaves and 58 genes in roots of the drought-resistant mutants were more highly expressed in one or more drought periods,these R2R3-MYB genes might play an important role in response to drought stress. The results of this study laid a foundation for further studying the function of the R2R3-MYB gene family in centipedegrass.

Key words: Centipedegrass, R2R3-MYB transcription factor, Bioinformatics, Drought stress, Expression pattern

中图分类号:

Q943.2

蒋宇佳, 于元平, 孙向一, 周敏, 吴春妍, 李玉华, 刘明稀. 假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2628-2641.

JIANG Yu-Jia, YU Yuan-Ping, SUN Xiang-Yi, ZHOU Min, WU Chun-Yan, LI Yu-Hua, LIU Ming-Xi. Identification of R2R3-MYB Gene Family and Analysis of its Expression Pattern in Centipedegrass under Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(9): 2628-2641.

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参考文献

[1] RIECHMANN J L,HEARD J,MARTIN G,et al. Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes[J]. Science,2000,290(5499):2105-2110
[2] LIPSICK J S. One billion years of Myb[J]. Oncogene,1996,13(2):223-235
[3] DUBOS C,STRACKE R,GROTEWOLD E,et al. MYB transcription factors in Arabidopsis[J]. Trends in Plant Science,2010,15(10):573-581
[4] STRACKE R,WERBER M,WEISSHAAR B. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current Opinion in Plant Biology,2001,4(5):447-456
[5] KLEMPNAUER K H,GONDA T J,BISHOP J M. Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb:the architecture of a transduced oncogene[J]. Cell,1982,31(2 Pt 1):453-463
[6] WESTON K M. The myb genes[J]. Seminars in Cancer Biology,1990,1(6):371-382
[7] PAZ-ARES J,GHOSAL D,WIENAND U,et al. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators[J]. The Embo Journal,1987,6(12):3553-3558
[8] KATIYAR A,SMITA S,LENKA S K,et al. Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis[J]. BMC Genomics,2012(13):544
[9] SMITA S,KATIYAR A,CHINNUSAMY V,et al. Transcriptional Regulatory Network Analysis of MYB Transcription Factor Family Genes in Rice[J]. Frontiers in Plant Science,2015(6):1157
[10] DU H,YANG S S,LIANG Z,et al. Genome-wide analysis of the MYB transcription factor superfamily in soybean[J]. BMC Plant Biology,2012(12):106
[11] XIE R,LI Y,HE S,et al. Genome-wide analysis of citrus R2R3MYB genes and their spatiotemporal expression under stresses and hormone treatments[J]. PLoS One,2014,9(12):e113971
[12] 冉舵.油桐MYB转录因子家族的鉴定与功能特征研究[D].长沙:中南林业科技大学,2019:11-12
[13] 居利香,雷欣,赵成志,等.辣椒MYB基因家族的鉴定及与辣味关系分析[J].园艺学报,2020,47(5):875-892
[14] MA D,CONSTABEL C P. MYB Repressors as Regulators of Phenylpropanoid Metabolism in Plants[J]. Trends in Plant Science,2019,24(3):275-289
[15] 潘新怡,史昆,龚攀,等.紫花苜蓿花青苷合成的转录组分析[J].草地学报,2021,29(5):866-875
[16] SCHUBERT R,DOBRITZSCH S,GRUBER C,et al. Tomato MYB21 Acts in Ovules to Mediate Jasmonate-Regulated Fertility[J]. Plant Cell,2019,31(5):1043-1062
[17] CAO X,XU L,LI L,et al. TcMYB29a,an ABA-Responsive R2R3-MYB Transcriptional Factor,Upregulates Taxol Biosynthesis in Taxus chinensis[J]. Frontiers in Plant Science,2022(13):804593
[18] 李春艳,钟华,杜利霞,等.白羊草BiMYB52基因的克隆及转基因拟南芥抗旱性表达分析[J].草地学报,2023,31(1):29-39
[19] LIU M,ZHANG Z,XU Z,et al. Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato[J]. Plant Cell Report,2021,40(1):43-58
[20] LI C N,NG C K Y,FAN L M. MYB transcription factors,active players in abiotic stress signaling[J]. Environmental and Experimental Botany,2015(114):80-91
[21] MEHRTENS F,KRANZ H,BEDNAREK P,et al. The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis[J]. Plant Physiology,2005,138(2):1083-1096
[22] SEO P J,PARK C M. MYB96-mediated abscisic acid signals induce pathogen resistance response by promoting salicylic acid biosynthesis in Arabidopsis[J]. New Phytologist,2010,186(2):471-483
[23] DING Z,LI S,AN X,et al. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana[J]. Journal of Genetics and Genomics,2009,36(1):17-29
[24] XU C,FU X,LIU R,et al. PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis[J]. Tree Physiology,2017,37(12):1713-1726
[25] SONG Q,KONG L,YANG X,et al. PtoMYB142,a poplar R2R3-MYB transcription factor,contributes to drought tolerance by regulating wax biosynthesis[J]. Tree Physiology,2022,42(10):2133-2147
[26] JIAN L,KANG K,CHOI Y,et al. Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis[J]. Plant Journal,2022,112(2):339-351
[27] 周寿荣.草坪地被与人类环境[M].成都:四川科技出版社,1996:82
[28] HANNA W W. Centipedegrass diversity and vnlnerabablity[J]. Crop Science,1995,35(2):332-334
[29] LI J,GUO H,ZONG J,et al. Genetic diversity in centipedegrass[Eremochloa ophiuroides (Munro) Hack.] [J]. Horticulture Research,2020(7):4
[30] 樊丛梅,孙洪海.优良草坪品种一假俭草[J].江苏林业科技,1990,17(4):49-50
[31] 任健,毛凯,范彦.假俭草的抗性[J].草业科学,1998,15(5):62-65
[32] 刘明稀,卢少云,郭振飞.假俭草抗旱变异体的筛选及其生理鉴定[J].草业学报,2012,21(1):126-132
[33] WANG J,ZI H,WANG R,et al. A high-quality chromosome-scale assembly of the centipedegrass[Eremochloa ophiuroides (Munro) Hack.]genome provides insights into chromosomal structural evolution and prostrate growth habit[J]. Horticulture Research,2021,8(1):201
[34] 鲁泽东.假俭草突变体抗旱生理分析及抗旱基因筛选[D].长沙:湖南农业大学,2020:14
[35] STRACKE R,ISHIHARA H,HUEP G,et al. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling[J]. The Plant Journal,2007,50(4):660-677
[36] 樊锦涛,蒋琛茜,邢继红,等.拟南芥R2R3-MYB家族第22亚族的结构与功能[J].遗传,2014,36(10):985-994
[37] 赵盼盼.番茄R2R3MYB转录因子家族鉴定及SlMYB41和SlMYB64基因功能研究[D].泰安:山东农业大学,2017:40-41
[38] 任明辉,张雨蓬,许涛,等.紫花苜蓿R2R3-MYB亚家族鉴定与干旱胁迫下的表达分析[J].草地学报,2023,31(4):972-983
[39] ZHONG R,LEE C,ZHOU J,et al. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis[J]. The Plant Cell,2008,20(10):2763-2782
[40] PARK M Y,KANG J Y,KIM S Y. Overexpression of AtMYB52 confers ABA hypersensitivity and drought tolerance[J]. Molecules and Cells,2011,31(5):447-454
[41] AOYAGID L N,LOPES-CAITAR V S,DE CARVALHO MCCG,et al. Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi[J]. Plant Science,2014,229(12):32-42
[42] COMINELLI E,SALA T,CALVI D,et al. Over-expression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability[J]. The Plant Journal,2008,53(1):53-64

假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析

Identification of R2R3-MYB Gene Family and Analysis of its Expression Pattern in Centipedegrass under Drought Stress

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