紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析

doi:10.11733/j.issn.1007-0435.2023.09.005

草地学报 ›› 2023, Vol. 31 ›› Issue (9): 2608-2620.DOI: 10.11733/j.issn.1007-0435.2023.09.005

紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析

唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍

内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010010

收稿日期:2023-04-26 修回日期:2023-07-10 出版日期:2023-09-15 发布日期:2023-10-07
通讯作者: 唐芳,E-mail:fta223@imau.edu.cn
作者简介:唐芳(1983-),女,汉族,贵州息烽人,博士,副教授,主要从事牧草栽培育种和植物-微生物互作研究,E-mail:fta223@imau.edu.cn
基金资助:
高层次留学人才回国工作资助项目，内蒙古自治区科技重大专项(2021ZD0031)；内蒙古农业大学高层次人才引进科研启动项目(NDGCC2016-18)资助

Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress

TANG Fang, MEI Ting, GAO Jia-he, WANG Jia-ni, SHI Feng-ling, GAO Cui-ping

College of Grassland, Resource and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010010, China

Received:2023-04-26 Revised:2023-07-10 Online:2023-09-15 Published:2023-10-07

摘要/Abstract

摘要： 甘油-3-磷酸酰基转移酶(Glycerol-3-phosphate acyltransferase,GPAT)是甘油生物合成的限速酶,参与多种脂类的生物合成途径,在植物生长发育和抗逆过程中具有重要作用。为了解GPAT基因在紫花苜蓿(Medicago sativa)应对非生物胁迫过程中的作用,本研究利用生物信息学方法共鉴定出73个紫花苜蓿MsGPATs基因。研究表明,MsGPAT基因在染色体上不均匀分布,大多数基因存在大片段复制现象。系统进化树分析将其分为3个亚组。基因结构和蛋白质保守基序分析表明MsGPAT基因家族成员多数含有2~3个外显子或11~12个外显子,至少含有1个保守基序。顺式作用元件分析表明不同MsGPATs基因含有不同的光、生长素和胁迫应答元件。基因表达谱分析显示,分别有10,5,18个MsGPATs基因积极响应高盐、高碱和混合盐碱胁迫,可作为紫花苜蓿耐盐碱研究的候选基因。

关键词: 紫花苜蓿, GPAT基因家族, 盐碱胁迫, 表达分析

Abstract: Glycerol-3-phosphate acyltransferase (GPAT) is the rate-limiting enzyme of glycerol biosynthesis,participates in the biosynthetic pathway of various lipids,and plays important roles in plant growth and stress resistance. To understand the role of GPAT genes in the response of alfalfa (Medicago sativa) to abiotic stress,a total of 73 MsGPATs genes in alfalfa were identified using bioinformatics methods in this study. Studies showed that the MsGPATs gene were unevenly distributed on chromosomes,and most genes exhibited segmental duplication. Phylogenetic tree analysis was classified into three subgroups. Gene structure and protein conservative motif analysis showed that most of the MsGPATs gene family members contained 2~3 exons or 11~12 exons,and at least one conserved motif. Promoter cis-acting elements analysis showed that different MsGPATs genes contained different light,auxin and stress response elements. Gene expression profiling analysis showed that a total of 10,5 and 18 MsGPATs genes actively responded to high salt,high alkali and mixed saline-alkali stress,respectively,which could be used as candidate genes for further research on saline and alkali tolerance in alfalfa.

Key words: Medicago sativa, GPAT gene family, Saline alkali stress, Expression analysis

中图分类号:

S541.9

唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍. 紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2608-2620.

TANG Fang, MEI Ting, GAO Jia-he, WANG Jia-ni, SHI Feng-ling, GAO Cui-ping. Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress[J]. Acta Agrestia Sinica, 2023, 31(9): 2608-2620.

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紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析

Identification and Expression Analysis of the GPAT Gene Family in Medicago sativa under Saline Alkali Stress

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