草地学报

草地学报 ›› 2015, Vol. 23 ›› Issue (6): 1310-1316.DOI: 10.11733/j.issn.1007-0435.2015.06.026

• 技术研发 • 上一篇    下一篇

陕西省苜蓿茎点霉叶斑病病原菌的鉴定

李洪建1, 李芳红1, 史健2, 呼天明1, 程积民1, 钱永华1, 焦锋1   

  1. 1. 西北农林科技大学动物科技学院, 陕西 杨凌 712100;
    2. 彬县西坡乡动物卫生监督所, 陕西 彬县 713500
  • 收稿日期:2014-09-01 修回日期:2015-01-14 出版日期:2015-12-15 发布日期:2016-02-01
  • 通讯作者: 钱永华, 焦锋
  • 作者简介:李洪建(1988-),男,四川遂宁人,硕士研究生,主要研究方向为牧草植物病理学,E-mail:suplihongjian@126.com
  • 基金资助:

    国家公益性行业(农业)科研专项经费项目(201303157);国家现代农业牧草产业技术体系(CRAS-35)资助

Identification of Pathogen Causing Phoma Leaf Spot on Medicago sativa in Shaanxi Province

LI Hong-jian1, LI Fang-hong1, SHI Jian2, Hu Tian-ming1, Cheng Ji-ming1, QIAN Yong-hua1, JIAO Feng1   

  1. 1. College of Animal Science and Technology, North West Agriculture and Forestry University, Yangling, Shaanxi Province 712100, China;
    2. Animal health inspection of Xipo country, Binxian, Shaanxi Province 713500, China
  • Received:2014-09-01 Revised:2015-01-14 Online:2015-12-15 Published:2016-02-01

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摘要：

近期发现了陕西省紫花苜蓿(Medicago sativa)一种苜蓿茎点霉叶斑病,为了准确鉴定该病害,开展了一系列病原鉴定试验。病叶片典型症状为叶尖上的深褐色斑点融合扩大呈“轮纹状”病斑;病原物的分生孢子无色透明,圆柱形,无隔单胞,大小为7×1.25 μm;以病原菌rDNA-ITS(核糖体DNA 内转录间隔区)序列的系统发育树的分析表明,病原菌与Phoma medicaginis var. Medicaginis(DQ109960)亲缘关系最近,NJ法中的相似性达到99%,UPGMA法中的相似性为98%;离体叶片接种验证病原菌具有致病性,结果表明:苜蓿茎点霉叶斑病的病原菌为苜蓿茎点霉(Phoma medicaginis var. Medicaginis)。

关键词: 苜蓿, 病原菌, rDNA-ITS, 苜蓿茎点霉, 离体叶片接种

Abstract:

Phoma Leaf spot is a serious leaf disease in alfalfa, which has happened in Shaanxi Province in recently. In order to find out the pathogen of this disease exactly, the diseased leaf typical symptom was that some sepia spots together expand like a "annulation" scab, and the conidia were hyaline, cylindrical, amerospore and unicellular, and the size was 7×1.25 μm. The phylogenetic tree of the pathogen nucleotide sequence of internal transcribed spacer (ITS) shared 99% similarity with that of Phoma medicaginis var. Medicaginis(DQ109960) in Neighbour-Joining(NJ) tree, or 98% similarity using unweighted pair-group method in arithmetic means(UPGMA ) tree. The inoculation of excised leaf tissue verified that the pathogen had pathogenicity. The results indicated that it was caused by Phoma medicaginis var. Medicaginis.

Key words: Medicago sativa, Pathogen, rDNA-ITS, Phoma Leaf spot, Excised leaf tissue inoculation

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