草地学报

草地学报 ›› 2017, Vol. 25 ›› Issue (2): 442-444.DOI: 10.11733/j.issn.1007-0435.2017.02.034

• 研究专报 • 上一篇    

草坪灰斑病的田间诊断与控制

张家齐1, 赵云梦2, 韩志勇2, 赵美琦2   

  1. 1. 河北省农林科学院植物保护研究所, 河北 保定 071000;
    2. 朝向集团草坪服务与研究中心, 北京 100193
  • 出版日期:2017-04-15 发布日期:2017-06-25
  • 通讯作者: 赵美琦,E-mail:zhaomeiqi2010@126.com
  • 作者简介:张家齐(1988-),女,浙江诸暨人,硕士,主要从事植物病害的研究,E-mail:jiaqi_321@yeah.net
  • 基金资助:

    973国家重点基础研究计划课题（2014CB138706）；教育部创新团队"草地农业系统耦合与管理"（IRT13019）；公益性行业（农业）科研专项（201403071-6）资助

Identification and Control of Turf Gray Leaf Spot

ZHANG Jia-qi1, ZHAO Yun-meng2, HAN Zhi-yong2, ZHAO Mei-qi2   

  1. 1. Plant Protection Institute, Hebei Academy of Agricutral and Forestry Science, Baoding, Hebei Province 071000, China;
    2. Forwardgroup Turf Service & Research Center, Beijing 100193, China
  • Online:2017-04-15 Published:2017-06-25

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摘要：

为了明确河南郑州某高尔夫球场的多年生黑麦草（Lolium perenne）上发生的病害种类，通过田间调查对草坪病害症状进行描述，对病样进行保湿培养并对分离到的病原物进行形态学观察、核糖体DNA-ITS序列分析。结果表明：该病原真菌的形态学与已报道的稻梨孢菌（Pyricularia grisea）相同，核糖体DNA-ITS序列测定分析该菌与稻梨孢菌的同源性达到100%，可以确定该球场的多年生黑麦草上的病害为稻梨孢菌引起的草坪灰斑病。

关键词: 草坪草, 多年生黑麦草, 稻梨孢菌, 灰斑病

Abstract:

This study was to define the symptom and causal agent of the Lolium perenne samples taken from a golf course in Zhengzhou, Henan Province. We observed the symptom of the disease and isolated the pathogen. The morphological characteristics and the DNA sequence of ribosomal ITS of the pathogen were studied. The pathogen was morphologically identified as Pyricularia grisea. The DNA sequence of ribosomal ITS analysis showed that it was 100% similar to that of P. grisea downloaded from Genbank. Thus we could confirm that the disease on Lolium perenne samples was gray leaf spot caused by P. Grisea.

Key words: Turfgrass, Lolium perenne, Pyricularia grisea, Gray leaf spot

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