棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探

doi:10.11733/j.issn.1007-0435.2023.05.005

草地学报 ›› 2023, Vol. 31 ›› Issue (5): 1314-1321.DOI: 10.11733/j.issn.1007-0435.2023.05.005

棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探

唐若怡¹, 王柏森², 董纯辛¹, 牛启尘¹, 尹淑霞¹

1. 北京林业大学草业与草原学院, 北京 100083;
2. 北京林业大学园林学院, 北京 100083

收稿日期:2022-11-25 修回日期:2023-01-22 出版日期:2023-05-15 发布日期:2023-05-31
通讯作者: 尹淑霞,E-mail:yinsx369@bjfu.edu.cn
作者简介:唐若怡(1997-)，女，汉族，山东枣庄人，硕士研究生，主要从事木霉生防作用研究，E-mail：TRY669@163.com
基金资助:
雄安新区科技创新专项(2022XAGG0100);国家林业和草原局委托项目(2021045001)资助

Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch

TANG Ruo-yi¹, WANG Bai-sen², DONG Chun-xin¹, NIU Qi-chen¹, YIN Shu-xia¹

1. School of Grassland Science, Beijing Forestry University, Beijing 100083, China;
2. School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Received:2022-11-25 Revised:2023-01-22 Online:2023-05-15 Published:2023-05-31

摘要/Abstract

摘要： 为探究棘孢木霉(Trichoderma asperellum)诱导多年生黑麦草(Lolium perenne L.)抗褐斑病的机理,本研究以多年生黑麦草为材料,通过棘孢木霉和玉蜀黍丝核菌(Rhizoctonia zeae)平板对抗、盆栽多年生黑麦草木霉灌根诱导后接种玉蜀黍丝核菌,检测棘孢木霉菌株152-42对玉蜀黍丝核菌引起的褐斑病的防治效果,分析其防病机理。结果表明:共培养7 d时棘孢木霉152-42对玉蜀黍丝核菌的抑制率达到100%;棘孢木霉152-42显著降低了盆栽多年生黑麦草褐斑病的发病率;相较于未经木霉诱导的染病叶片,木霉诱导处理的染病叶片超氧化物歧化酶活性显著升高,丙二醛含量显著降低,抗病相关基因PR-1和PR-5的表达量显著提高。因此,棘孢木霉152-42不仅能显著抑制玉蜀黍丝核菌生长,而且可以诱导多年生黑麦草通过提高抗病相关酶活性,调控抗病相关基因的表达等提高抗病性,是一种极具开发潜力的草坪病害生防真菌。

关键词: 棘孢木霉, 玉蜀黍丝核菌, 多年生黑麦草, 生物防治, 褐斑病

Abstract: In order to explore the resistance mechanism of perennial ryegrass (Lolium perenne L.),induced by Trichoderma asperellum strain 152-42 against to brown patch,this study used both plateco-culture test and pot infection test to check the biocontrol effects of T. asperellum strain 152-42 against perennial ryegrass brown patch caused by Rhizoctonia zeae,and further analyze the resistance mechanism. The results showed that T. asperellum 152-42 could totally inhibit the R. zeae when they co-cultured at 7 days on PDA medium. Moreover,T. asperellum 152-42 significantly reduced the incidence of brown patch to perennial ryegrass. Compared to treatments that inoculated R. zeae but non-treated with T. asperellum 152-42,the diseased leaves treated with T. asperellum 152-42 displayed a significant increment of the superoxide dismutase(SOD) activity and a significant decrease of the Malondialdehyde (MDA) content,and higher expression of disease-resistance genes PR-1 and PR-5. In conclusion,T. asperellum 152-42 was able not only to significantly inhibit the growth of R. zeae,but also induced perennial ryegrass to improve resistance against the brown patch by increasing the activity of related enzymes and regulating related genes of disease-resistance. Therefore,the T. asperellum 152-42 is an excellent potential biocontrol fungus that can be used to control turf diseases of brown patch.

Key words: Trichoderma asperellum, Rhizoctonia zeae, Perennial ryegrass, Biological control, Turf brown patch

中图分类号:

S543+ .6

唐若怡, 王柏森, 董纯辛, 牛启尘, 尹淑霞. 棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探[J]. 草地学报, 2023, 31(5): 1314-1321.

TANG Ruo-yi, WANG Bai-sen, DONG Chun-xin, NIU Qi-chen, YIN Shu-xia. Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch[J]. Acta Agrestia Sinica, 2023, 31(5): 1314-1321.

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棘孢木霉152-42诱导多年生黑麦草抗褐斑病的机理初探

Preliminary Study on Resistance Mechanism of Perennial Ryegrass Induced by Trichoderma asperellum 152-42 Against Brown Patch

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