草地学报

草地学报 ›› 2019, Vol. 27 ›› Issue (3): 620-627.DOI: 10.11733/j.issn.1007-0435.2019.03.014

• 研究论文 • 上一篇    下一篇

铝胁迫对紫花苜蓿不同种质材料的影响及综合评价

姜娜1, 唐敏2, 韩博1, 任健1, 许文花1, 毕玉芬1, 马向丽1   

  1. 1. 云南农业大学动物科学技术学院, 云南 昆明 650201;
    2. 云南农业大学园林园艺学院, 云南 昆明 650201
  • 收稿日期:2019-01-18 修回日期:2019-05-21 出版日期:2019-06-15 发布日期:2019-07-19
  • 通讯作者: 马向丽, 毕玉芬
  • 作者简介:姜娜(1995-),女,安徽滁州人,硕士研究生,主要从事牧草种质资源与利用研究,Email:18655035016@163.com
  • 基金资助:
    国家自然科学基金项目（31601997）；云南农业大学研究生课程案例库建设项目（A2001225）；云南省重点研发计划项目子课题（2018BB001-03）资助

Effects of Aluminum Stress on Different Germplasm Materials of Alfalfa and Its Comprehensive Evaluation

JIANG Na1, TANG Min2, HAN Bo1, REN Jian1, XU Wen-hua1, BI Yu-fen1, MA Xiang-li1   

  1. 1. College of Animal Science and Technology of Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
    2. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China
  • Received:2019-01-18 Revised:2019-05-21 Online:2019-06-15 Published:2019-07-19

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摘要： 铝毒是紫花苜蓿（Medicago sativa）在南方酸性土壤地区种植的主要限制性因素之一。筛选能够在酸性富铝化土壤中生长优良的苜蓿材料，对于紫花苜蓿在南方大面积推广具有重要意义。本研究选用27份云南紫花苜蓿为材料，研究100 mg·kg-1浓度铝胁迫处理下供试材料株高、脯氨酸、根系活力以及有机酸含量的变化。结果表明，铝对苜蓿生长具有明显的抑制作用，且不同材料反应敏感性不同。铝对供试苜蓿株高和根系活力有明显抑制作用；铝处理下苜蓿叶片中游离脯氨酸、有机酸含量显著积累（P<0.05），所有供试材料的相对脯氨酸含量均大于100%，但不同材料相对有机酸含量表现差异较大。采用聚类分析及隶属函数法综合评价供试苜蓿材料的耐受性，以15，12，14，13号材料表现较优，可用于选育适应酸性富铝化土壤的苜蓿品种。

关键词: 苜蓿, 耐铝性, 有机酸, 综合评价

Abstract: Aluminum toxicity is one of the main limiting factors for alfalfa (Medicago sativa) planting in acid soil areas in the South. It is very important for alfalfa to be widely used in southern China to select alfalfa materials which can grow well in acid-aluminized soil. In this study,27 Yunnan alfalfa resources were selected as experimental materials to study the changes of plant height,proline,root activity and organic acid content under 100 mg·kg-1 aluminum concentration. The results showed that aluminum inhibited the growth of alfalfa,and different materials have different sensitivities. Aluminum inhibited plant height and root activity of alfalfa.The content of free proline and organic acid in alfalfa leaves under aluminum treatment was significantly increased (P<0.05),The relative proline content of all tested materials was greater than 100%,but the relative organic acid content of different materials is quite different. Clustering analysis and membership function method were used to comprehensively evaluate the tolerance of the tested materials. The results showed that NO.15,NO.12,NO.14 and NO.13 were superior and could be used to breed alfalfa varieties suitable for acid-aluminized soil.

Key words: Alfalfa, Aluminum stress, Organic acids, Comprehensive assessment

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