二月兰种带真菌分离与鉴定

doi:10.11733/j.issn.1007-0435.2020.02.006

草地学报 ›› 2020, Vol. 28 ›› Issue (2): 338-349.DOI: 10.11733/j.issn.1007-0435.2020.02.006

二月兰种带真菌分离与鉴定

肖博文¹, 于丝雨¹, 冯伟², 段廷玉¹

1. 兰州大学草地农业生态系统国家重点实验室, 兰州大学农业农村部草牧业创新重点实验室, 兰州大学草地农业教育部工程研究中心, 兰州大学草地农业科技学院, 甘肃兰州 730020;
2. 河北省农林科学院农业资源环境研究所, 河北石家庄 050051

收稿日期:2019-10-12 修回日期:2020-01-02 出版日期:2020-04-15 发布日期:2020-04-11
通讯作者: 冯伟
作者简介:肖博文(1996-),女,四川仁寿人,硕士研究生,主要从事微生物研究,E-mail:xiaobw15@lzu.edu.cn
基金资助:
国家绿肥产业技术体系（CARS-22）资助

Isolation and Identification of Seed Borne Fungi on Orychophragmus violaceus

XIAO Bo-wen¹, YU Si-yu¹, FENG Wei², DUAN Ting-yu¹

1. State Key Laboratory of Grassland Agro-ecosystems;Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs;Engineering Research Center of Grassland Industry, Ministry of Education;College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China;
2. Institute of Agro-resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei Province 050051, China

Received:2019-10-12 Revised:2020-01-02 Online:2020-04-15 Published:2020-04-11

摘要/Abstract

摘要： 种带真菌是植物病害的重要传播途径，本研究以重要绿肥作物二月兰（Orychophragmus violaceus）为研究对象，通过室内分离培养、形态学和分子学鉴定，旨在明确二月兰种带真菌种类，探讨其种带真菌与田间病害发生的相关性。试验结果获得二月兰种带真菌5属7种，分别为链格孢属链格孢菌（Alternaria alternata），芸薹生链格孢（A. brassicicola），细极链格孢（A. tenuissima），附球菌属黑附球菌（Epicoccum nigrum），镰刀菌属变红镰刀菌（Fusarium incarnatum），哈氏霉属顶孢哈氏霉（Harzia acremonioides），细基格孢属（Ulocladium sp.）真菌；其中芸薹生链格孢是导致田间二月兰叶斑病的病原。通过高通量测序得到二月兰种带真菌25属30种，包括常见致病菌芸苔链格孢、十字花科白粉菌（Erysiphe cruciferarum）、孔状短小茎点霉（Phoma exigua var. exigua）、粉红单端孢（Trichothecium roseum）和大丽轮枝菌（Verticillium dahliae）等。可见，二月兰种带真菌多为常见致病菌，应进一步开展其致病性研究，关注种带真菌与田间病害发生的相关性，据此研发防治技术。

关键词: 二月兰, 绿肥, 种带真菌, 高通量测序技术

Abstract: Seed borne fungus is an important spread way of plant disease. The present study was aimed to identify the seed borne fungi of Orychophragmus violaceus, an important green manure crop,by using morphological and molecular testing methods. The relationship of seed borne fungus and the disease O. violaceus in field were discussed. In this study,seven seed borne species belong to five genera were isolated from the seeds of O. violaceus and identified based on morphological and molecular tests. The fungi are Alternaria alternata,A. brassicicola,A. tenuissima,Epicoccum nigrum,Fusarium incarnatum,Harzia acremonioides and Ulocladium sp. Thirty species belong to twenty-five genera of fungi were identified by high-throughput sequencing method. Including common pathogens such as A. brassicae,Erysiphe cruciferarum,Phoma exigua var. exigua,Trichothecium roseum and Verticillium dahlia,most of the isolated seed borne fungi are common pathogens of many crops,and further pathogenicity research should be carried out. The correlation between the fungi species and field diseases should be pay attention to,and the prevention and control technology should be developed accordingly.

Key words: Orychophragmus violaceus, Green manure, Seed borne fungi, High-throughput sequencing

中图分类号:

S553+.9

肖博文, 于丝雨, 冯伟, 段廷玉. 二月兰种带真菌分离与鉴定[J]. 草地学报, 2020, 28(2): 338-349.

XIAO Bo-wen, YU Si-yu, FENG Wei, DUAN Ting-yu. Isolation and Identification of Seed Borne Fungi on Orychophragmus violaceus[J]. Acta Agrestia Sinica, 2020, 28(2): 338-349.

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二月兰种带真菌分离与鉴定

Isolation and Identification of Seed Borne Fungi on Orychophragmus violaceus

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