丛枝菌根真菌与根瘤菌对3种豆禾混播植物种间互作的影响

doi:10.11733/j.issn.1007-0435.2021.04.002

草地学报 ›› 2021, Vol. 29 ›› Issue (4): 644-654.DOI: 10.11733/j.issn.1007-0435.2021.04.002

丛枝菌根真菌与根瘤菌对3种豆禾混播植物种间互作的影响

潘越, 周冀琼^*, 郭川, 杨喆, 刘琳, 闫艳红, 赵艳兰, 刘珊, 孙飞达

四川农业大学草业科技学院,四川成都 611130

收稿日期:2020-10-21 修回日期:2020-12-06 发布日期:2021-06-02
通讯作者: * E-mail:jiqiong_zhou@sicau.edu.cn
作者简介:潘越(1999-),女,四川德阳人,本科生,主要从事草地管理与生态研究,E-mail:9734309@qq.com
基金资助:
国家自然科学青年基金项目(31901379);四川省科技厅应用基础项目(2019YJ0431);新疆维吾尔自治区区域协同创新专项(科技援疆计划2019E0211)资助

Arbuscular Mycorrhizal Fungi and Rhizobia Regulate Plant Interspecific Interaction of Three Legumes and Grasses Species

PAN Yue, ZHOU Ji-qiong^*, GUO Chuan, YANG Zhe, LIU Lin, YAN Yan-hong, ZHAO Yan-lan, LIU Shan, SUN Fei-da

College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China

Received:2020-10-21 Revised:2020-12-06 Published:2021-06-02
Contact: * E-mail:jiqiong_zhou@sicau.edu.cn

摘要/Abstract

摘要： 丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)和根瘤菌是两类重要的土壤微生物,可与宿主植物形成互利共生关系。本研究通过对多年生黑麦草(Lolium perenne)、鸭茅(Dactylis glomerata)和白三叶(Trifolium repens)构建的混播组合接种AMF和根瘤菌,探讨两种微生物对3种植物竞争关系的调控机制。结果表明,混播处理中鸭茅和多年生黑麦草的株高及生物量显著高于各自的单播处理,白三叶的株高及生物量显著低于其单播处理;接种AMF提高了混播中白三叶的株高、生物量、根系结瘤数,但AMF抑制了群落中优势种鸭茅的生长,缓解了鸭茅对白三叶的竞争排除作用。综上所述,双接种AMF和根瘤菌进一步提高了混播中白三叶的地下生物量及结瘤数,促进白三叶生长固氮并调节了3种植物的种间竞争关系,从而有利于豆禾混播群落功能性和稳定性的维持。

关键词: 豆禾混播, 丛枝菌根真菌, 根瘤菌, 种间竞争

Abstract: Arbuscular mycorrhizal fungi (AMF) and rhizobia are well-known microbes which can influence plant coexistence and grassland productivity,due to their complementary roles in meeting plant nutritional needs. However,the effect of these two microbial symbionts on the component of a plant community varies with the mycorrhizal dependence of different species. Our study was conducted to measure the effects of AMF,rhizobia and their potential interactions on the productivity and interspecific competition of different plant species. Trifolium repens,Lolium perenne and Dactylis glomerata were used to establish legumes-grasses mixture,associated with AMF/rhizobia or both AMF and rhizobia. The plant height and biomass of T.repens were greater in monocultures than polycultures,whereas the plant height and biomass of L.perenne and D.glomerata were greater in polycultures than their monocultures respectively. Compared to non-AMF inoculated treatments,AMF inoculation significantly promoted plant height,aboveground biomass and root nodulation of T.repens,but decreased the biomass of D.glomerata in polycultures. This resulted in the decreased competitive exclusion from dominant grass,D.glomerata. Co-inoculated with AMF and rhizobia typically altered interspecies competitive relationship between legumes and grasses,significantly increased the root development and N₂ fixing capability of T.repens,and enhanced soil nutrient availability for neighbouring grasses. Our study promise that these two microbial symbioses are critical drivers of productivity and stability of legumes-grasses mixture.

Key words: Legumes-grasses mixture, Arbuscular mycorrhizal fungi, Rhizobia, Interspecific competition

中图分类号:

S939.11+4

潘越, 周冀琼, 郭川, 杨喆, 刘琳, 闫艳红, 赵艳兰, 刘珊, 孙飞达. 丛枝菌根真菌与根瘤菌对3种豆禾混播植物种间互作的影响[J]. 草地学报, 2021, 29(4): 644-654.

PAN Yue, ZHOU Ji-qiong, GUO Chuan, YANG Zhe, LIU Lin, YAN Yan-hong, ZHAO Yan-lan, LIU Shan, SUN Fei-da. Arbuscular Mycorrhizal Fungi and Rhizobia Regulate Plant Interspecific Interaction of Three Legumes and Grasses Species[J]. Acta Agrestia Sinica, 2021, 29(4): 644-654.

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参考文献

[1] 谢开云,赵云,李向林,等. 豆-禾混播草地种间关系研究进展[J]. 草业学报,2013,22(3):284-296
[2] Bever J D,Dickie I A,Facelli E,et al. Rooting theories of plant community ecology in microbial interactions[J]. Trends in Ecology & Evolution,2010,25(8):468-478
[3] Reynolds,Heather L,Packer,et al. Grassroots ecology:plant-microbe-soil interactions as drivers of plant community structure and dynamics[J]. Ecology,2003,84(9):2281-2291
[4] 何丹,李向林,万里强,等. 施用尿素当年对退化天然草地物种地上生物量和重要值的影响[J]. 草业学报,2009,18(3):154-158
[5] Larimer A L,Bever J D,Clay K. The interactive effects of plant microbial symbionts:a review and meta-analysis[J]. Symbiosis,2010,51(2):139-148
[6] Bolan N S. A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants[J]. Plant and Soil,1991,134(2):189-207
[7] 贾永,宋福强. 丛枝菌根(AM)对根瘤菌趋化作用研究[J]. 微生物学通报,2008(5):743-747
[8] 任爱天,杨洁晶,马春晖,等. 丛枝菌根真菌与豆科植物共生的研究进展[J]. 黑龙江畜牧兽医,2014(11):51-53
[9] Hayman D S. Mycorrhizae of nitrogen-fixing legumes[J]. World Journal of Microbiology and Biotechnology,1986,2(1):121-145
[10] Wagg C,Jansa J,Stadler M,et al. Mycorrhizal fungal identity and diversity relaxes plant-plant competition[J]. Ecology,2011,92(6):1303-1313
[11] Jia Y,Gray V M,Straker C J,et al. The influence of Rhizobium and arbuscular mycorrhizal fungi on nitrogen and phosphorus accumulation by vicia faba[J]. Annals of Botany,2004,94(2):251-258
[12] Ceulemans T,Merckx R,Hens M,et al. A trait-based analysis of the role of phosphorus vs. nitrogen enrichment in plant species loss across North-west European grasslands[J]. Journal of Applied Ecology,2011,48(5):1155-1163
[13] Coskun D,Britto D T,Shi W,et al. How Plant Root Exudates Shape the Nitrogen Cycle[J]. Trends in Plant Science,2017,22(8):661-673
[14] Larimer A L,Clay K,Bever J D. Synergism and context dependency of interactions between arbuscular mycorrhizal fungi and rhizobia with a prairie legume[J]. Ecology,2014,95(4):1045-1054
[15] Marcel G A,Heijden V D,Susanne,et al. A widespread plant-fungal-bacterial symbiosis promotes plant biodiversity,plant nutrition and seedling recruitment[J]. The ISME journal,2016,10(2):389-99
[16] Vogelsang K M,Reynolds H L,Bever J D. Mycorrhizal fungal identity and richness determine the diversity and productivity of a tallgrass prairie system[J]. New Phytologist,2006,172(3):554-562
[17] Hartnett D C,Wilson G W T. The role of mycorrhizas in plant community structure and dynamics:lessons from grasslands[J]. Plant and Soil,2002,244(1-2):319-331
[18] Hartnett D C,Wilson G W T. Mycorrhizae influence plant community structure and diversity in tallgrass prairie[J]. Ecology,1999,80(4):1187-1195
[19] Wilson G W T,Hartnett D C,Rice C W. Mycorrhizal-mediated phosphorus transfer between tallgrass prairie plants Sorghastrum nutans and Artemisia ludoviciana[J]. Functional Ecology,2006,20(3):427-435
[20] Bauer J T,Kleczewski M N,Bever J D,et al. Nitrogen-fixing bacteria,arbuscular mycorrhizal fungi,and the productivity and structure of prairie grassland communities[J]. Oecologia,2012,170(4):1089-1098
[21] Grman E,Allen J,Galloway E,et al. Inoculation with remnant prairie soils increased the growth of three native prairie legumes but not necessarily their associations with beneficial soil microbes[J]. Restoration Ecology,2020,28:S393-S399
[22] 王幼珊,张淑彬,张美庆. 中国丛枝菌根真菌资源与种质资源[M]. 北京:中国农业出版社,2012:153-154
[23] Maltz M R,Treseder K K. Sources of inocula influence mycorrhizal colonization of plants in restoration projects:A meta-analysis[J]. Restoration Ecology,2015,23(5):625-634
[24] Johnson N C,Wilson G W T,Bowker M A,et al. Resource limitation is a driver of local adaptation in mycorrhizal symbioses[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(5):2093-2098
[25] Hoeksema J D,Chaudhary V B,Gehring C A,et al. A meta-analysis of context-dependency in plant response to inoculation with mycorrhizal fungi[J].Ecology Letters,2010,13(3):394-407
[26] 王宏信,冷凝,剧春晖,等. 菌根真菌与根瘤菌联合作用对降香黄檀幼苗根系构型及养分吸收的影响[J]. 江苏农业科学,2018,46(13):120-124
[27] Walder,Niemann,Natarajan,et al. Mycorrhizal networks:common goods of plants shared under unequal terms of trade[J]. Plant Physiology,2012,159(2):789-797
[28] Koske R E,Gemma J N. A modified procedure for staining roots to detect va mycorrhizas[J]. Elsevier,1989,92(4):486-505
[29] Mcgonigle T P,Miller M H,Evans D G,et al. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi[J]. New Phytol,1990,115(3):495-501
[30] 鲍士旦. 土壤农业化学分析方法[M]. 北京:中国农业出版社,2000:264-270
[31] Menge J A,Johnson E L V,Platt R G. Mycorrhizal dependency of several citrus cultivars under three nutrient regimes[J]. New Phytologist,1978,81(3):553-559
[32] 朱红惠,姚青,龙良坤,等. 不同氮形态对AM真菌孢子萌发和菌丝生长的影响[J]. 菌物学报,2004(4):590-595
[33] 俞洪燕,叶贞榜,罗绪强. 不同叶龄施肥对玉米苗期农艺性状的影响[J]. 耕作与栽培,2015(6):1-3,12
[34] Helgason T,Merryweather J W,Denison J,et al. Selectivity and functional diversity in arbuscular mycorrhizas of co-occurring fungi and plants from a temperate deciduous woodland[J]. Journal of Ecology,2002,90(2):371-384
[35] Hage-Ahmed K,Krammer J,Steinkellner S. The intercropping partner affects arbuscular mycorrhizal fungi and fusarium oxysporum f. sp. lycopersici interactions in tomato[J]. Mycorrhiza,2013,23(7):543-550
[36] Bever J D. Negative feedback within a mutualism:host-specific growth of mycorrhizal fungi reduces plant benefit. Proceedings[J]. Biological sciences,2002,269(1509):2595-2601
[37] Stein C,Cornelia Rissmann,Hempel S,et al. Interactive effects of mycorrhizae and a root hemiparasite on plant community productivity and diversity[J]. Oecologia,2009,159(1):191-205
[38] 石伟琦. 丛枝菌根真菌对内蒙古草原大针茅群落的影响[J]. 生态环境,2010,19(2):344-349
[39] Scheublin T R,Richard S.P.Van Logtestijn,Marcel G.A. Van Der Heijden. Presence and identity of arbuscular mycorrhizal fungi influence competitive interactions between plant species[J]. Journal of Ecology,2007,95(4):631-638
[40] Bahadur A,Jin Z,Long X,et al. Arbuscular mycorrhizal fungi alter plant interspecific interaction under nitrogen fertilization[J]. European Journal of Soil Biology,2019,93:103094
[41] Sheng M,Tang M,Chen H,et al. Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress[J]. Mycorrhiza,2008,18(6-7):287-296
[42] Endlweber K,Scheu S. Interactions between mycorrhizal fungi and collembola:effects on root strueture of competing plant species[J]. Biology and Fertility of Soils,2007,43(6):741-749
[43] Wang X,Pan Q,Chen F,et al. Effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on soybean growth as related to root architecture and availability of N and P[J]. Mycorrhiza,2011,21(3):173-181
[44] 韩可,孙彦,张昆,等. 接种不同根瘤菌对紫花苜蓿生产力的影响[J]. 草地学报,2018,26(3):639-644
[45] Lowther W L,Patrick H N. Spread of Rhizobium and Bradyrhizobium in soil[J]. Soil Biology and Biochem,1993,25(5):607-612
[46] Zhang W,Li X G,Sun K,et al. Mycelial network-mediated rhizobial dispersal enhances legume nodulation[J]. The ISME Journal,2020,14(4):1015-1029
[47] Bisseling T,Geurts R. Specificity in legume nodule symbiosis[J]. Science,2020,369(6504):620-62
[48] 张福锁,申建波. 根际微生态系统理论框架的初步构建[J]. 中国农业科技导报,1999(4):15-20
[49] Heijden M G A V D,Bakker R,Verwaal J,et al. Symbiotic bacteria as a determinant of plant community structure and plant productivity in dune grassland[J]. FEMS microbiology ecology,2006,56(2):178-187
[50] 白梨花,斯日格格,曹丽霞,等. 丛枝菌根对牧草与草地生态系统的重要作用及其研究展望[J]. 草地学报,2013,21(2):214-221

丛枝菌根真菌与根瘤菌对3种豆禾混播植物种间互作的影响

Arbuscular Mycorrhizal Fungi and Rhizobia Regulate Plant Interspecific Interaction of Three Legumes and Grasses Species

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