外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响

doi:10.11733/j.issn.1007-0435.2021.04.001

草地学报 ›› 2021, Vol. 29 ›› Issue (4): 635-643.DOI: 10.11733/j.issn.1007-0435.2021.04.001

• 研究论文 • 下一篇

外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响

朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲^*

甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2020-10-18 修回日期:2020-11-05 发布日期:2021-06-02
通讯作者: * E-mail:mahl@ gsau.edu.cn
作者简介:朱瑞婷(1995-),女,甘肃天水人,硕士研究生,主要从事草坪生物学研究,E-mail:1258151161@qq.com
基金资助:
甘肃省高校创新基金项目(2020-054A)资助

Effect of Exogenous 2,4-Epibrassinolide on the Chlorophyll Metabolism of Kentucky Bluegrass under Cd Stress

ZHU Rui-ting, NIU Kui-ju, ZHANG Di-jun, YANG Yang, SONG Yun, MA Hui-ling^*

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Centers for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2020-10-18 Revised:2020-11-05 Published:2021-06-02
Contact: * E-mail:mahl@ gsau.edu.cn

摘要/Abstract

摘要： 本研究以草地早熟禾(Poa pratensis)‘午夜’幼苗为试验材料,研究镉(Cadmium,Cd)胁迫下及不同浓度(5,50,100 nM)的2,4-表油菜素内酯(2,4-Epibrassinolide,EBR)对0.5 mM CdCl₂胁迫下草地早熟禾叶片的叶绿素代谢途径的影响。研究结果表明,Cd胁迫1 d后可诱导PpPBGD,PpCHLD,PpCHLI和PpPOR基因的下调表达和PpCLH,PpPPH,PpPAO和PpRCCR基因的上调表达。50 nM的EBR可显著提高Cd胁迫下原卟啉Ⅳ、镁-原卟啉Ⅳ、原叶绿素酸酯、叶绿素a和总叶绿素的含量,到Cd胁迫6 d时,这些指标与未胁迫对照无显著差异,这可能与外源EBR诱导的叶绿素生物合成基因的上调表达和降解通路的下调表达有关。因此,外源EBR可通过诱导叶绿素生物合成通路基因的上调表达来缓解Cd胁迫引起的叶绿素中间产物转化的受阻程度,以及通过诱导降解通路基因的下调表达来减缓叶绿素的降解,进而使得Cd胁迫下草地早熟禾叶片叶绿素含量维持相对较高的水平。

关键词: 草地早熟禾, 镉胁迫, 表油菜素内酯, 叶绿素代谢, 基因表达

Abstract: In the present study,Kentucky bluegrass ‘Midnight’ seedlings were used as experimental materials to study the chlorophyll metabolism pathways of leaves under Cd stress and different concentrations of 2,4-epibrassinolide (EBR:5,50,100 nM) with 0.5 mM CdCl₂ stress. The results showed that Cd stress induced the down-regulated expression of PpPBGD,PpCHLD, PpCHLI and PpPOR genes and the up-regulated expression of PpCLH,PpPPH,PpPAO and PpRCCR genes after 1 day. The application of 50 nM EBR significantly increased protoporphyrin Ⅳ,Mg-protoporphyrin Ⅳ,protochlorophyllide,chlorophyll a and total chlorophyll contents within Cd stress. After 6 days of Cd stress,the content of these indicators of 50nM EBR-treated seedlings had no significant difference with the control,which might be attributed to the upregulation of chlorophyll biosynthetic genes and degradative genes.The results indicated that the alleviative effect on the decline of chlorophyll content were associated with that the exogenous EBR induced the upregulation expression of genes involved in chlorophyll biosynthetic genes and the downregulation expression of genes involved in chlorophyll degradation.

Key words: Kentucky bluegrass, Cd stress, 2,4-Epibrassinolide, Chlorophyll metabolism, Gene expression

中图分类号:

Q945.78

朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.

ZHU Rui-ting, NIU Kui-ju, ZHANG Di-jun, YANG Yang, SONG Yun, MA Hui-ling. Effect of Exogenous 2,4-Epibrassinolide on the Chlorophyll Metabolism of Kentucky Bluegrass under Cd Stress[J]. Acta Agrestia Sinica, 2021, 29(4): 635-643.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.04.001

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I4/635

参考文献

[1] 綦峥,齐越,杨红,等. 土壤重金属镉污染现状、危害及治理措施[J]. 食品安全质量检测学报,2020,11(7):2286-2294
[2] Rizwan M,Ali S,Adrees M,et al. A critical review on effects,tolerance mechanisms and management of cadmium in vegetables[J]. Chemosphere,2017(182):90-105
[3] 陈亚奎,葛登文,卢滇楠. 镉污染土壤的植物修复技术[J]. 环境生态学,2020,2(9):92-98
[4] Ali H,Khan E,Sajad MA. Phytoremediation of heavy metals—Concepts and applications[J]. Chemosphere,2013(91):869-881
[5] Hu Y,Xu L,Tian S,et al. Site-specific regulation of transcriptional responses to cadmium stress in the hyperaccumulator,Sedum alfredii:based on stem parenchymal and vascular cells[J]. Plant Molecular Biology,2019,99(4-5):347-362
[6] 曾星,李伟亚,陈章,等. 龙葵修复镉污染土壤的研究进展[J]. 草业科学,2019,36(5):1308-1316
[7] Zhu H,Ai H,Cao L,et al. Transcriptome analysis providing novel insights for Cd-resistant tall fescue responses to Cd stress[J]. Ecotoxicology and Environmental Safety,2018(160):349-356
[8] 鲜靖苹,柴澍杰,王勇,等. 镉胁迫对草地早熟禾生长与生理代谢的影响[J]. 核农学报,2019,33(1):176-186
[9] 徐佩贤. 高羊茅和草地早熟禾对镉的耐受能力和解毒机制研究[D]. 上海:上海交通大学,2014:96-97
[10] Xu P,Wang Z. A comparison study in cadmium tolerance and accumulation in two cool-season turfgrasses and Solanum nigrum L.[J]. Water Air & Soil Pollution,2014,225(5):1938
[11] Planas-Riverola A,Gupta A,Betegón-Putzeet I,et al. Brassinosteroid signaling in plant development and adaptation to stress[J]. Development,2019,146(5):151894
[12] 覃凤飞,李志华,刘信宝,等. 外源2,4表油菜素内酯对越夏期高温与弱光胁迫下紫花苜蓿生长和光合性能的影响[J]. 草业学报,2020,29(9):146-160
[13] 普布卓玛,罗艺岚,高金柱,等. 2,4表-油菜素内酯对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响[J]. 草地学报,2019,27(3):547-552
[14] 陈燕华,王亚梁,朱德峰,等. 外源油菜素内酯缓解水稻穗分化期高温伤害的机理研究[J]. 中国水稻科学,2019,33(5):457-466
[15] 丁丹阳,张璐翔,朱智威,等. 叶面喷施2,4-表油菜素内酯对烟草抗旱性的影响[J]. 中国烟草科学,2018,39(4):50-57
[16] 寇江涛. 外源2,4-表油菜素内酯对NaCl胁迫下燕麦幼苗光合特性的影响[J]. 华北农学报,2020,35(2):79-87
[17] 俞明宏,王力明,刘继,等. 表油菜素内酯对镉胁迫下番茄幼苗生长及镉累积的影响[J].中国土壤与肥料,2020(3):151-156
[18] 刘大林,杨俊俏,刘兆明,等. 镉、铅胁迫对草地早熟禾幼苗生理的影响[J]. 草业科学,2015,32(2):224-230
[19] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000:134-137
[20] Hodgins R R,Van Huystee R B. Rapid Simultaneous Estimation of Protoporphyrin and Mg-Porphyrins in Higher Plants[J]. Journal of Plant Physiology,1986,125(3-4):311-323
[21] 赵春旭,马祥,董文科,等. 低温胁迫下不同青海野生草地早熟禾的转录组比较分析[J]. 草地学报,2020,28(2):305-318
[22] Niu K J,MA H L. The positive effects of exogenous 5-aminolevulinic acid on the chlorophyll biosynthesis,photosystem and calvin cycle of Kentucky bluegrass seedlings in response to osmotic stress[J]. Environmental and Experimental Botany,2018(155):260-271
[23] Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR[J]. Methods,2002,25(4):402-408
[24] 周际海,程坤,郜茹茹,等. 土壤镉污染对香樟幼苗光合和生理特性的影响[J]. 林业科学,2020,56(6):193-201
[25] Anjum S A,Xie X Y,Wang L C,et al. Morphological,physiological and biochemical responses of plants to drought stress[J]. African Journal of Agricultural Research,2011,6(9):2026-2032
[26] Jespersen D,Zhang J,Huang B R. Chlorophyll loss associated with heat-induced senescence in bentgrass[J]. Plant Science,2016(249):1-12
[27] Dalal V K,Tripathy B C. Modulation of chlorophyll biosynthesis by water stress in rice seedlings during chloroplast biogenesis[J]. Plant Cell & Environment,2012,35(9):1685-1703
[28] 向丽霞,胡立盼,孟森,等. 叶面喷施亚精胺对高温胁迫下番茄叶绿素合成代谢的影响[J]. 西北植物学报,2020,40(5):846-851
[29] 张丽,徐志然,胡晓辉,等. 叶面喷施亚精胺对盐碱胁迫下番茄幼苗生长及其叶绿素合成前体含量的影响[J]. 西北植物学报,2015,35(1):125-130
[30] Kaewsuksaeng S. Chlorophyll Degradation in Horticultural Crops[J]. Walailak Journal of Science and Technology,2011,8(1):987-1005
[31] Tanaka R,Tanaka A. Tetrapyrrole Biosynthesis in Higher Plants[J]. Annual Review of Plant Biology,2007,58(1):321
[32] Akram N A,Ashraf M. Regulation in Plant Stress Tolerance by a Potential Plant Growth Regulator,5-Aminolevulinic Acid[J]. Journal of Plant Growth Regulation,2013,32(3):663-679
[33] Turan S,Tripathy B C. Salt-stress induced modulation of chlorophyll biosynthesis during de-etiolation of rice seedlings[J]. Physiologia Plantarum,2015,153(3):477-491
[34] Stefan H,Bernhard K. Chlorophyll breakdown in higher plants[J]. Biochimica Et Biophysica Acta,2010,1807(8):977-988
[35] Gan L,Han L,Yin S,et al. Chlorophyll Metabolism and Gene Expression in Response to Submergence Stress and Subsequent Recovery in Perennial Ryegrass Accessions Differing in Growth Habits[J]. Journal of Plant Physiology,2020(251):153195
[36] Schelbert,Silvia,Aubry,et al. Pheophytin Pheophorbide Hydrolase (Pheophytinase) Is Involved in Chlorophyll Breakdown during Leaf Senescence in Arabidopsis[J]. Plant Cell,2009,21(3):767-785
[37] Ma N,Ma X,Li A,et al. Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO[J]. Plant Molecular Biology Reporter,2012,30(5):1237-1245
[38] Guo S,Shu S,Zhou H,et al. Exogenous spermidine delays chlorophyll metabolism in cucumber leaves (Cucumis sativus L.) under high temperature stress[J]. Acta Physiologiae Plantarum,2016,38(9):224
[39] Xiao H J,Liu K K,Li D W,et al. Cloning and characterization of the pepper CaPAO gene for defense responses to salt-induced leaf senescence[J]. BMC biotechnology,2015(15):100
[40] Peng R,Sun W,Jin X,et al. Analysis of 2,4-epibrassinolide created an enhancement tolerance on Cd toxicity in Solanum nigrum L[J]. Environmental Science and Pollution Research,2020,27(14):16784-16797
[41] Planas-Riverola A,Gupta A,Betegón-Putze I,et al. Brassinosteroid signaling in plant development and adaptation to stress[J]. Development,2019,146(5):151894
[42] Wang Y,Cao J J,Wang K X,et al. BZR1 mediates brassinosteroid-induced autophagy and nitrogen starvation tolerance in tomato[J]. Plant Physiology,2018,172(2):671-685
[43] Lucas M D,Salomé P. PIFs get brright:Phytochrome interacting factors as integrators of light and hormonal signals[J]. New Phytologist,2014,202(4):1126-1141
[44] Moon J,Zhu L,Shen H,et al. PIF1 directly and indirectly regulates chlorophyll biosynthesis to optimize the greening process in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2008,105(27):9433-9438

外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响

Effect of Exogenous 2,4-Epibrassinolide on the Chlorophyll Metabolism of Kentucky Bluegrass under Cd Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[2]	金一锋, 陈阳, 齐欣, 高岩松, 金忠民, 赵清峰, 王琦. 草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析[J]. 草地学报, 2021, 29(7): 1397-1405.
[3]	朱琨, 郑桂华, 刘丽杰, 周璐, 李珊珊, 范震宇, 钟玥, 尼尼. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报, 2021, 29(7): 1430-1435.
[4]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[5]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[6]	张杨杨, 李希铭, 高鹏, 宋桂龙. 不同浓度镉胁迫下6种草本植物的耐性及富集特征的比较[J]. 草地学报, 2021, 29(6): 1265-1276.
[7]	王文静, 麻冬梅, 赵丽娟, 马巧利. 2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响[J]. 草地学报, 2021, 29(6): 1363-1368.
[8]	其美拉姆, 普布卓玛, 崔彤彤, 张新飞, 姜惠娜, 付娟娟, 呼天明. 外源钙对低温胁迫下西藏野生垂穗披碱草生理及相关基因表达的影响[J]. 草地学报, 2021, 29(5): 919-928.
[9]	许超, 何承刚, 牟兰, 毕玉芬, 姜华. 干热胁迫下紫花苜蓿Rubisco羧化酶和活化酶活性变化及其基因表达的研究[J]. 草地学报, 2021, 29(2): 228-233.
[10]	孔海明, 李开源, 高双红, 孙娈姿. 根瘤菌对紫花苜蓿生物修复镉污染功能的影响[J]. 草地学报, 2021, 29(12): 2763-2768.
[11]	王升升, 段珍, 张吉宇. 发根农杆菌介导的白花草木樨毛状根转化体系的建立[J]. 草地学报, 2021, 29(11): 2591-2599.
[12]	高娅楠, 韩烈保, 许立新. 乙烯利对干旱胁迫下草地早熟禾抗氧化酶基因表达的影响[J]. 草地学报, 2021, 29(10): 2200-2213.
[13]	张然, 马祥, 朱瑞婷, 牛奎举, 赵春旭, 马晖玲. 青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析[J]. 草地学报, 2020, 28(6): 1508-1518.
[14]	赵春旭, 张然, 牛奎举, 朱瑞婷, 王勇, 马祥, 马晖玲. 青海野生草地早熟禾响应低温胁迫的代谢组学研究[J]. 草地学报, 2020, 28(4): 904-914.
[15]	普布卓玛, 其美拉姆, 李丹丹, 多吉顿珠, 张靖, 孙运府, 苗彦军, 付娟娟, 呼天明. 外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响[J]. 草地学报, 2020, 28(3): 589-596.