铝胁迫对狗牙根根系生长和营养元素的影响

doi:10.11733/j.issn.1007-0435.2022.04.019

草地学报 ›› 2022, Vol. 30 ›› Issue (4): 936-942.DOI: 10.11733/j.issn.1007-0435.2022.04.019

• 研究论文 • 上一篇

铝胁迫对狗牙根根系生长和营养元素的影响

段宏利^1,2, 蒋凌雁¹, 陈志坚², 黄春琼²

1. 海南大学热带作物学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室, 海南海口 571101

收稿日期:2021-09-27 修回日期:2021-11-14 发布日期:2022-04-25
通讯作者: 蒋凌雁,E-mail:lyjiang@hainanu.edu.cn;黄春琼,E-mail:huangchunqiong44@163.com
作者简介:段宏利(1997-),女,新疆乌鲁木齐人,硕士研究生,主要从事植物抗逆研究,E-mail:496379001@qq.com
基金资助:
2019年海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(No.2019RC313);科技基础资源调查专项"中国南方草地牧草资源调查"(No.2017FY100600);现代产业技术体系牧草岗位科学家经费(CARS-34)资助

Effects of Aluminum Stress on Root Growth and Contents of Nutrient Elements of Cynodon dactylon

DUAN Hong-li^1,2, JIANG Ling-yan¹, CHEN Zhi-jian², HUANG Chun-qiong²

1. Hainan University, Haikou, Hainan Province 570228, China;
2. Tropical Crops Genetic Resources Institute/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan Province 571101, China

Received:2021-09-27 Revised:2021-11-14 Published:2022-04-25

摘要/Abstract

摘要： 为了探究狗牙根(Cynodon dactylon(L.) Pers.)耐铝种质及敏铝种质根系生长和营养元素对铝胁迫的响应。本研究以狗牙根耐铝种质A22和敏铝种质A326作为试验材料,在500 μM AlCl₃(pH值4.0)溶液中设计了3个处理时间(0 h,12 h,24 h),在24 h处理时间点设计了4个铝处理浓度,分别为0(CK),250,500,750 μM AlCl₃(pH值4.0)。通过对相对根长、根系苏木精染色、根系铝含量和10种营养元素的测定,结果表明,在不同铝胁迫下,A22根系伸长受抑制的程度均小于A326;A326根系苏木精染色深于A22,且根系铝含量高于A22;营养元素P,Ca,Mg,K,Fe,Mn,Cu,Zn和B的含量在A22和A326中都受到了不同程度的抑制,且与两个种质中根系铝含量呈负相关。初步了解铝胁迫下狗牙根在营养生理上的耐铝机制,有利于在酸性土壤中更好地利用狗牙根,为培育耐酸铝的草品种提供初步的理论基础。

关键词: 铝胁迫, 狗牙根, 营养元素

Abstract: In this study,aluminum-tolerant germplasm A22 and aluminum-sensitive germplasm A326 were used as experimental materials.The plants of Cynodon dactylon(L.) Pers.were treated in 500 μ M AlCl₃ solution for 0 h,12 h and 24 h or in 0,250,500,750 μ M AlCl₃ for 24 h.The stress responses of Al-tolerant and Al-sensitive C.dactylon were studied by measuring relative root length,hematoxylin staining and contents of Al and other 10 nutrient elements.The results showed that the inhibition of root elongation of A22 was less than that of A326 under different aluminum stress treatments.The roots of A326 were stained deeper than that of A22 by hematoxylin;and the aluminum content in roots of A326 was higher than that of A22.The accumulation of P,Ca,Mg,K,Fe,Mn,Cu,Zn and B were inhibited by Al stress and negatively correlated with Al in both germplasms.This study provides data to understand the mechanism of Al tolerance of C.dactylon under Al stress,which is helpful to make better use of C.dactylon in acid soil and provide theoretical basis for the development of al tolerance cultivars.

Key words: Aluminum stress, Cynodon dactylon (L.) Pers., Nutrient elements

中图分类号:

S311

段宏利, 蒋凌雁, 陈志坚, 黄春琼. 铝胁迫对狗牙根根系生长和营养元素的影响[J]. 草地学报, 2022, 30(4): 936-942.

DUAN Hong-li, JIANG Ling-yan, CHEN Zhi-jian, HUANG Chun-qiong. Effects of Aluminum Stress on Root Growth and Contents of Nutrient Elements of Cynodon dactylon[J]. Acta Agrestia Sinica, 2022, 30(4): 936-942.

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铝胁迫对狗牙根根系生长和营养元素的影响

Effects of Aluminum Stress on Root Growth and Contents of Nutrient Elements of Cynodon dactylon

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