瑞香狼毒根提取物对荞麦桃蚜的毒力及其作用机制

doi:10.11733/j.issn.1007-0435.2022.05.017

草地学报 ›› 2022, Vol. 30 ›› Issue (5): 1165-1170.DOI: 10.11733/j.issn.1007-0435.2022.05.017

瑞香狼毒根提取物对荞麦桃蚜的毒力及其作用机制

高玉莲¹, 王贻卉¹, 常静¹, 康鹏¹, 马崇勇², 韩志勇³, 李海平¹

1. 内蒙古农业大学园艺与植物保护学院, 内蒙古呼和浩特 010019;
2. 内蒙古自治区草原工作站, 内蒙古呼和浩特 010020;
3. 内蒙古自治区乌拉特前旗林业和草原局草原工作站, 内蒙古乌拉特前旗 014400

收稿日期:2021-11-04 修回日期:2021-12-19 出版日期:2022-05-15 发布日期:2022-06-06
通讯作者: 李海平,E-mail:lihaiping5820@hotmail.com
作者简介:高玉莲(1995-),女,汉,山西省平遥县人,硕士研究生,主要从事农业昆虫与害虫防治研究,E-mail:287391458@qq.com
基金资助:
草原生态保护修复治理——毒害草监测与防控项目资助

Mechanism and Toxicity of Root Extract from Stellera chamaejasme on Myzus persicaein Buckwheat

GAO Yu-lian¹, WANG Yi-hui¹, CHANG Jing¹, KANG Peng¹, MA Chong-yong², HAN Zhi-yong³, LI Hai-ping¹

1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Inner Mongolia Grassland Station, Hohhot, Inner Mongolia 010020, China;
3. Urad Front Banner Grassland Station in Inner Mongolia, Urad Front Banner, Inner Mongolia 014400, China

Received:2021-11-04 Revised:2021-12-19 Online:2022-05-15 Published:2022-06-06

摘要/Abstract

摘要： 蚜虫是荞麦(Fagopyrum esculentumMoench)花期的主要害虫，对荞麦产量影响很大，本论文研究了不同浓度瑞香狼毒(Stellera chamaejasme L.)根提取物对荞麦桃蚜的杀虫活性以及对其体内2种靶标酶和3种解毒酶活性的影响。结果表明：瑞香狼毒根石油醚提取物对桃蚜有较强的毒杀作用，24 h对桃蚜的致死中浓度LC₅₀为1 243.7 mg·L^-1。桃蚜经提取物处理24 h后，其体内乙酰胆碱酯酶活性受到抑制，最高抑制率为21.7%；腺苷三磷酸酶活性升高，Na⁺-K⁺-ATPase和Ca²⁺-Mg²⁺-ATPase活性在提取物浓度为4 000 mg·L^-1时最大，显著高于未处理桃蚜两种酶活性，分别是未处理的12.0和1.8倍。瑞香狼毒根提取物提高了桃蚜体内解毒酶——谷胱甘肽-S-转移酶和细胞色素P₄₅₀的活性，降低了羧酸酯酶活性。综上，瑞香狼毒根石油醚提取物对桃蚜有很好的毒杀作用，提取物可影响桃蚜的靶标酶和解毒酶活性，干扰其正常生理生化活动。

关键词: 荞麦, 桃蚜, 瑞香狼毒, 毒力, 作用机制

Abstract: Aphid (Myzus persicae) is the main pest in buckwheat (Fagopyrum esculentum) flowering period,and the yield of buckwheat can be affected greatly. The toxicity of root extract from Stellera chamaejasme L. on aphids were tested in this paper. Effect of root extract from S. chamaejasme on two target enzyme activities and three detoxification enzymes of M. persicae were measured in the same time. The results showed that the petroleum ether extract of S. chamaejasme root had toxic effect on M. persicae seriously,and the median lethal concentration (LC₅₀) was 1 243.7 mg·L^-1 in 24 h. The activity of acetylcholinesterase(AChE) in M. persicaewas inhibited after treatment for 24 h,and the highest inhibition rate was 21.7%. However,the activity of ATPase in M. persicae increased,the activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase were the largest at the extract concentration of 4 000 mg·L^-1. The activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase were higher than that of the control significantly,which was 12.0 and 1.8 times of the control,respectively in 4 000 mg·L^-1. The activities of detoxification enzymes glutathione-S-transferase(GSTs) and cytochrome P₄₅₀ were both induced by the extract. The activity of carboxylesterase (CarE) was inhibited. In conclusion,the root petroleum ether extract of S. chamaejasme was toxic to M. persicae in buckwheat,and the target enzymes and detoxification enzymes of M. persicae could be affected by the extract. The normal physiological and biochemical activities of M. persicae was interfered by the extract.

Key words: Buckwheat, Myzus persicae, Stellera chamaejasme, Toxicity, Mechanism

中图分类号:

S435

高玉莲, 王贻卉, 常静, 康鹏, 马崇勇, 韩志勇, 李海平. 瑞香狼毒根提取物对荞麦桃蚜的毒力及其作用机制[J]. 草地学报, 2022, 30(5): 1165-1170.

GAO Yu-lian, WANG Yi-hui, CHANG Jing, KANG Peng, MA Chong-yong, HAN Zhi-yong, LI Hai-ping. Mechanism and Toxicity of Root Extract from Stellera chamaejasme on Myzus persicaein Buckwheat[J]. Acta Agrestia Sinica, 2022, 30(5): 1165-1170.

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瑞香狼毒根提取物对荞麦桃蚜的毒力及其作用机制

Mechanism and Toxicity of Root Extract from Stellera chamaejasme on Myzus persicaein Buckwheat

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