草地学报

草地学报 ›› 2023, Vol. 31 ›› Issue (4): 1057-1063.DOI: 10.11733/j.issn.1007-0435.2023.04.014

• 研究论文 • 上一篇    

紫花苜蓿胚性愈伤组织诱导与增殖的研究

谭璐娜, 彭嘉文, 许环宇, 张瀚文, 夏厚胤, 苗愉祺, 崔祎琳, 苑旺, 马志超, 同雨涛, 高景慧   

  1. 西北农林科技大学草业与草原学院, 陕西 杨陵 712100
  • 收稿日期:2022-09-26 修回日期:2022-12-02 发布日期:2023-04-28
  • 通讯作者: 高景慧,E-mail:gaojinghui@nwsuaf.edu.cn
  • 作者简介:谭璐娜(2000-),女,汉族,重庆万州人,硕士研究生,主要从事遗传资源与牧草育种研究,E-mail:tln0905@163.com;彭嘉文(2000-),女,汉族, 河南南阳人,硕士研究生,主要从事遗传资源与牧草育种研究,E-mail:pengjiawen2021@nwsuaf.edu.cn
  • 基金资助:
    陕西省重点研发一般项目(2019NY-074)资助

Induction and Proliferation of Embryogenic Callus of Alfalfa

TAN Lu-na, PENG Jia-wen, XU Huan-yu, ZHANG Han-wen, XIA Hou-yin, MIAO Yu-qi, CUI Yi-lin, YUAN Wang, MA Zhi-chao, TONG Yu-tao, GAO Jing-hui   

  1. College of Grass and Grassland, Northwest A & F University, Yangling, Shaanxi Province 712100, China
  • Received:2022-09-26 Revised:2022-12-02 Published:2023-04-28

PDF

摘要： 为在不同栽培苜蓿(Medicago sativa)品种上，建立稳定、快速且高效的组织培养体系，本试验以品种'游客’、'Paola’和'Sardi7’为研究材料，探究了苜蓿外植体、激素配比及金属离子(Cu2+、Ag+)，对其胚性愈伤组织的诱导效果。结果表明：下胚轴为诱导愈伤组织的最佳外植体；MS+1 mg·L-12,4-D+0.2 mg·L-1 KT为培养基时'游客’和'Paola’胚性愈伤诱导率分别为24.7%，16.7%；MS+2 mg·L-12,4-D+0.2 mg·L-1 KT为培养基时'Sardi7’胚性愈伤诱导效果最佳为27.3%；1 μmol·L-1 和5 μmol·L-1 Cu2+均能显著促进'Sardi7’'游客’和'Paola’愈伤组织的诱导；5 μmol·L-1 Ag+显著诱导'游客’和'Paola’形成胚性愈伤组织，诱导率分别提高25.3%,46.7%(P<0.05)。本研究探索了诱导苜蓿愈伤组织的最佳外植体及其培养条件、金属离子促进不同苜蓿品种再生的作用，为突破同源四倍体栽培苜蓿品种基因型上的限制、提高胚性愈伤组织出愈率提供了思路。

关键词: 紫花苜蓿, 下胚轴, 金属离子, 胚性愈伤, 显微观察

Abstract: In order to establish a stable,rapid and efficient tissue culture system on different cultivated alfalfa (Medicago sativa) varieties,this experiment took the varieties 'Tourist’'Paola’ and 'Sardi7’ as the research materials,investigated the effects of alfalfa explants,hormones ratio and metal ions (Cu2+,Ag+) on induction of embryonic callus. The results showed that hypocotyl was the best explants to induce callus. When MS+1 mg·L-1 2,4-D+0.2 mg·L-1 KT were cultured,embryogenic callus induction rates of 'Tourist’ and 'Paola’ were 24.7% and 16.7% respectively. And the embryogenic callus induction effect of 'Sardi7’ was 27.3% when MS+2 mg·L-1 2,4-D +0.2 mg·L-1 KT were cultured. 1 μmol·L-1 and 5 μmol·L-1 Cu2+ could promote callus induction of 'Sardi7’,'Tourist’ and 'Paola’ significantly. 5 μmol·L-1 Ag+ significantly induced embryogenic callus of 'Tourist’ and 'Paola’,and the induction rates increased by 25.3% and 46.7%,respectively (P<0.05). In this study,we explored the best explants to induce alfalfa callus and their culture conditions,as well as the role of metal ions in promoting regeneration of different alfalfa varieties,which provided a solution for breaking through the genotypic restriction of autotetraploid cultivated alfalfa varieties and improving the healing rate of embryonic callus.

Key words: Alfalfa (Medicago sativa), Hypocotyl, Metal ions, Embryogenic callus, Microscopic observation

中图分类号: