硒引发对不同品种紫花苜蓿种子抗氧化特性的影响

doi:10.11733/j.issn.1007-0435.2022.08.013

草地学报 ›› 2022, Vol. 30 ›› Issue (8): 2037-2044.DOI: 10.11733/j.issn.1007-0435.2022.08.013

• 研究论文 • 上一篇

硒引发对不同品种紫花苜蓿种子抗氧化特性的影响

王勃, 王聪聪, 夏方山, 陈奕霖, 赵萍, 朱慧森

山西农业大学草业学院, 山西太谷 030801

收稿日期:2021-12-27 修回日期:2022-03-16 发布日期:2022-09-01
通讯作者: 夏方山,E-mail:dqlxfs8583@163.com
作者简介:王勃(1998-),女,汉族,山西晋城人,硕士研究生,主要从事草种子科学研究,E-mail:wangbo10898@163.com
基金资助:
山西农业大学“十四五”生物育种工程项目（YZGC134）；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题（2021KF0303）；晋中市科技重点研发计划（Y202012）资助

Influence of Selenium Priming on the Antioxidant Characteristics in Different Varieties of Alfalfa Seeds

WANG Bo, WANG Cong-cong, XIA Fang-shan, CHEN Yi-lin, ZHAO Ping, ZHU Hui-sen

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2021-12-27 Revised:2022-03-16 Published:2022-09-01

摘要/Abstract

摘要： 为探讨外源硒引发对不同品种紫花苜蓿(Medicago sativa)种子抗氧化能力的影响，试验采用浓度为0.5 mmol·L^-1的亚硒酸钠溶液对12个品种的紫花苜蓿种子进行了0(CK)，3，6，9和12 h的引发处理后，分析了其丙二醛含量及超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶活性的变化规律。结果表明：12个品种的紫花苜蓿种子抗氧化能力对外源硒引发的响应存在明显差异，大多数品种的紫花苜蓿种子在短时间(3~6 h)硒引发下仍保持较高的抗氧化能力，而仅有'偏关苜蓿’种子的抗氧化能力在长时间(12 h)硒引发时仍显著高于CK(P<0.05)，其他品种紫花苜蓿种子的抗氧化能力则均显著低于CK(P<0.05)。从抗氧化能力变化来看，12个紫花苜蓿品种中以'偏关苜蓿’最适宜采用引发技术进行富硒草产品生产，而'WL343HQ’，'WL363HQ’，'WL656HQ’，'WL319HQ’和'WL903HQ’紫花苜蓿品种则不适宜。

关键词: 紫花苜蓿, 硒, 种子引发, 品种, 抗氧化性能

Abstract: To explore the influence of exogenous selenium on the antioxidant ability of different cultivar alfalfa (Medicago sativa) seed,seeds of 12 alfalfa cultivars were primed with sodium selenite solution for 0 (control),3,6,9 and 12 h with 0.5 mmol·L^-1,then malondialdehyde content,the activity of superoxide dismutase,catalase,ascorbic acid peroxidase and glutathione reductase were analyzed. The results indicated that the antioxidant ability of seed was obviously different in response to priming with exogenous selenium in the 12 varieties of alfalfa seed. After selenium priming a short time (3~6 h),most varieties of alfalfa seed could maintain a high level of antioxidant ability. However,after selenium priming a long time (12 h),the antioxidant ability of alfalfa 'Pianguan' was still significantly higher than control (P<0.05),while other varieties of alfalfa seeds were significantly lower than control (P<0.05). In the terms changes in their antioxidant ability,alfalfa 'Pianguan' was the most suitable to use the technology of seed priming in producing selenium-rich products,but the alfalfa varieties of 'WL343HQ','WL363HQ','WL656HQ','WL319HQ' and 'WL903HQ' were not unsuitable.

Key words: Alfalfa, Selenium, Seed priming, Variety, Oxidation resistance

中图分类号:

S541+.9

王勃, 王聪聪, 夏方山, 陈奕霖, 赵萍, 朱慧森. 硒引发对不同品种紫花苜蓿种子抗氧化特性的影响[J]. 草地学报, 2022, 30(8): 2037-2044.

WANG Bo, WANG Cong-cong, XIA Fang-shan, CHEN Yi-lin, ZHAO Ping, ZHU Hui-sen. Influence of Selenium Priming on the Antioxidant Characteristics in Different Varieties of Alfalfa Seeds[J]. Acta Agrestia Sinica, 2022, 30(8): 2037-2044.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2022.08.013

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I8/2037

参考文献

[1] SILVA V M,BOLETA E H M,MARTINS J T,et al. Agronomic biofortification of cowpea with selenium:effects of selenate and selenite applications on selenium and phytate concentrations in seeds[J]. Journal of the Science of Food and Agriculture,2019,99(13):5969-5983
[2] 程贝,韩如冰,刘家齐,等.不同品种紫花苜蓿种子耐硒能力研究[J].江苏农业科学,2020,48(12):150-155
[3] DU B,LUO H,HE L,et al. Rice seed priming with sodium selenite:Effects on germination,seedling growth and biochemicalattributes[J]. Scientific Reports,2019,9(1):4311
[4] 侯青光,韦林汕,卢亚妮,等.硒肥不同喷施时期和种类对玉米产量、品质及硒和重金属含量的影响[J].西南农业学报,2021,34(9):1900-1907
[5] GU Y,QIU Y,WEI X,et al. Characterization of seleniumcontaining polysaccharides isolated from seleniumenriched tea and its bioactivities[J]. Food Chemistry,2020(316):126371
[6] 贾亚琴,董飞,杨峰,等.喷施硒肥对黑小麦籽粒产量及硒含量的影响[J].麦类作物学报,2021,41(10):1-6
[7] 张祎雯,董畅茹,刘小强,等.硒对水稻吸收转运镉、砷和汞影响机制的研究进展[J].生物学杂志,2021,38(5):101-109
[8] 蒋曦龙,乔丹彤,李晓靖,等.叶面过量施肥对玉米产量、硒和矿物质营养元素含量的影响[J].核农学报,2021,35(12):2841-2849
[9] ETTEIEB S,MAGDOULI S,ZOLFAGHARI M,et al. Monitoring and analysis of selenium as an emerging contaminant in mining industry:A critical review[J]. Science of the Total Environment,2020(698):134339
[10] FAROOQ M,WAHID A,SIDDIQUE K H M. Micronutrient application through seed treatments-areview[J]. Journal of Soil Science and Plant Nutrition,2012,12(1):125-142
[11] IQBAL S,FAROOQ M,CHEEMA S A,et al. Boron seed priming improves the seedling emergence,growth,grain yield and grain biofortification of bread wheat[J]. International Journal of Agriculture and Biology,2017,19(1):177-182
[12] CHEN K,ARORA R. Priming memory invokes seed stress-tolerance[J]. Environment and Experimental Botany,2013(94):33-45
[13] MOULICK D,SANTRA S C,GHOSH D. Effect of selenium induced seed priming on arsenic accumulation in rice plant and subsequent transmission in human food chain[J]. Ecotoxicology and Environmental Safety,2018(152):67-77
[14] 夏方山,王聪聪,李红玉,等.硒引发对紫花苜蓿种子抗氧化性能的影响[J].草地学报,2021,29(3):472-477
[15] ARUN M N,HEBBAR S S,BHANUPRAKAS K,et al. Seed priming improves irrigation water use efficiency,yield and yield components of summer cowpea under limited water conditions[J]. Legume Research,2017,40(5):864-871
[16] 郭孝,胡华锋,介晓磊,等.日粮中添加富硒钴苜蓿草粉对家兔饲料转化率和肉品质的影响[J].家畜生态学报,2017,38(12):29-33
[17] 赵娇阳,李小艳,朱慧森,等.偏关苜蓿种子萌发及植株再生体系优化[J].草地学报,2020,28(4):947-955
[18] 姚泽英,李军,宋连昭,等.垂穗披碱草与紫花苜蓿不同时期交叉混播效果研究[J].草地学报,2020,28(5):1454-1459
[19] 田春丽,李斌,刘芳,等.硒锌和富啡酸配施对紫花苜蓿叶片抗氧化酶活性及产量的影响[J].草地学报,2018,26(4):935-941
[20] XIA F,WANG C,LI Y,et al. Influence of priming with exogenous selenium on seed vigour of alfalfa (Medicago sativa L.)[J]. Legume Research,2021,44(9):1124-1127
[21] 胡华锋,介晓磊,郭孝,等.基施硒肥对不同生育期紫花苜蓿营养含量及分配的影响[J].草地学报,2014,22(4):871-877
[22] 胡华锋,刘太宇,郭孝,等.基施硒肥对不同生育期紫花苜蓿吸收、转化及利用硒的影响[J].草地学报,2015,23(1):101-106
[23] 胡华锋,王彦华,李明,等.基施硒肥对不同生育期紫花苜蓿营养含量及分配的影响[J].草地学报,2015,23(6):1347-1350
[24] ANON. International rules for seed testing:The germination test[M]. Zurich:International Seed Testing Association,2019:25
[25] XIA F S,WANG Y C,ZHU H S,et al. Influence of priming with exogenous boron on the seed vigor of alfalfa (Medicago sativa L.)[J]. Legume Research,2019(42):795-799
[26] KIBINZA S,VINEL D,CȎME D,et al. Sunflower seed deterioration as related to moisture content during ageing,energy metaboli sm and active oxygen species scavenging[J]. Physiologia Plantarum,2006(128):496-506
[27] RAO K V M,SRESTY T V S. Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses[J]. Plant Science,2000(157):113-128
[28] AEBI H E. Catalase. In methods of enzymatic analysis[M]. Weinheim:Verlag Chemie,1986:273-286
[29] NAKANO Y,ASADA K. Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplast[J]. Plant and Cell Physiology,1981(22):867-880
[30] MADAMANCHI N R,ALSCHER R G. Metabolic bases for differences in sensitivity of two pea cultivars to sulfur dioxide[J]. Plant Physiology,1991,97(1):88-93
[31] BAILLY C,BENAMAR A,CORBINEAU F,et al. Changes in malondialdehyde content and superoxide dismutase,catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging[J]. Physiologia Plantarum,1996(97):104-111
[32] 张士敏,朱慧森,赵娇阳,等.不同硒源及浓度对偏关苜蓿种子萌发及物质转化的影响[J].草地学报,2022,30(1):100-107
[33] 彭琪,何红花,范陈斌,等.硒对紫花苜蓿种子萌发及幼苗生长的影响[J].种子,2020,39(6):1-8
[34] 于章龙,赵智勇,刘瑞,等.运黑14207硒赋存形态及抗氧化特性研究[J].麦类作物学报,2022,42(3):1-7
[35] WOCH W,HAWRYLAK-NOWARK B. Selected antioxidant properties of alfalfa,radish,and white mustard sprouts biofortified with selenium[J]. Acta Agrobotanica,2019,72(2):1768
[36] 向安妮,许爽,鞠红梅,等.遏蓝菜硒蛋白的纯化、结构表征及抗氧化活性研究[J].西北农业学报,2022,31(3):1-11
[37] 张岩松,黄皓婷,韩莹琰,等.硒浓度对高温胁迫下生菜抗氧化酶活性的影响[J].北京农学院学报,2021,36(4):39-43
[38] BAI B,WANG Z,GAO L,et al. Effects of selenite on the growth of alfalfa(Medicago sativa L. cv. Sadie 7)and related physiological mechanisms[J]. Acta Physiologiae Plantarum,2019,41(6):78
[39] PILON-SMITS E A H. On the ecology of selenium accumulation in plants[J]. Plants,2019,8(7):1-17
[40] 代惠萍,崔科飞,贾根良,等.紫花苜蓿叶片对硒胁迫的生理响应研究[J].北方园艺,2016(17):64-67

硒引发对不同品种紫花苜蓿种子抗氧化特性的影响

Influence of Selenium Priming on the Antioxidant Characteristics in Different Varieties of Alfalfa Seeds

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李红玉, 王雅聪, 曾佳, 王聪聪, 董宽虎, 夏方山. 外源H₂O₂引发对燕麦种胚细胞AsA-GSH循环的影响[J]. 草地学报, 2022, 30(7): 1668-1674.
[2]	徐洪雨, 李钰莹. 基于KEGG通路分析紫花苜蓿的冷适应过程[J]. 草地学报, 2022, 30(7): 1721-1730.
[3]	余如刚, 王雪茄, 王国良, 徐智明, 高晴晴, 杜雪玲, 张宇龙. 紫花苜蓿品种苗期耐盐性分析及评价指标筛选[J]. 草地学报, 2022, 30(7): 1781-1789.
[4]	阚海明, 庞卓, 陈超, 邹俊亮, 张国芳, 武菊英. 北京西北浅山区退化草地植被恢复对土壤微生物群落多样性的影响[J]. 草地学报, 2022, 30(6): 1350-1358.
[5]	李莉萍, 李旭漉, 张晓捷, 谷丽丽, 张博. 紫花苜蓿atpA基因的克隆及表达模式分析[J]. 草地学报, 2022, 30(6): 1413-1421.
[6]	马淑敏, 焦婷, 师尚礼, 秦伟娜, 王正文, 赵生国, 祁娟. 混合型乳酸菌制剂对不同品种青饲玉米青贮品质的影响[J]. 草地学报, 2022, 30(6): 1558-1568.
[7]	毛培, 申书恒, 蒲军, 娄可可, 周强, 王增裕, 孙娟, 刘志鹏. 基于SCoT标记分析紫花苜蓿遗传多样性的取样数量[J]. 草地学报, 2022, 30(5): 1110-1121.
[8]	魏江文, 刘磊, 王森山, 陈丽霞, 蒋明君, 王庚浩, 徐亨昊. “抗”“感”苜蓿品种对豌豆蚜适应性及体内酶活的影响[J]. 草地学报, 2022, 30(5): 1171-1177.
[9]	孙延亮, 刘选帅, 李生仪, 赵俊威, 马春晖, 张前兵. 新疆石河子地区不同秋眠级紫花苜蓿生产性能的综合评价[J]. 草地学报, 2022, 30(5): 1227-1236.
[10]	昝看卓, 刘丛, 张燕燕, 阿山江·伊米尼江, 白婕, 龙明秀, 王可珍, 屈洋, 何树斌. 关中地区紫花苜蓿，小麦及玉米根际土壤理化性质研究[J]. 草地学报, 2022, 30(5): 1246-1252.
[11]	张海铉, 霍文婕, 陈雷, 刘强, 张拴林, 王聪, 许庆方, 郭刚. 添加剂对紫花苜蓿青贮品质及小肠可吸收氨基酸的影响[J]. 草地学报, 2022, 30(5): 1302-1309.
[12]	王静, 刘晓静, 童长春, 汪雪. 不同紫花苜蓿品种的氮效率比较及差异机制研究[J]. 草地学报, 2022, 30(4): 889-900.
[13]	刘东霞, 杨莉, 宋连昭, 杨志敏, 王芳, 刘贵河. 三种镰孢菌对不同苜蓿品种致病性的比较研究[J]. 草地学报, 2022, 30(4): 909-918.
[14]	昝看卓, 阿山江·伊米尼江, 刘丛, 谭凯, 白婕, 何树斌, 王可珍, 屈洋, 龙明秀. 不同秋眠类型紫花苜蓿根际与非根际土壤理化性质及化学计量特征[J]. 草地学报, 2022, 30(4): 957-965.
[15]	王芳, 李伟, 刘鑫, 李文辰, 赵金波, 张智慧, 杨曌. 紫花苜蓿根际土壤细菌群落对腐熟牛粪响应[J]. 草地学报, 2022, 30(3): 603-611.