接种丛枝菌根真菌对不同生育期牧草生长的影响

doi:10.11733/j.issn.1007-0435.2024.04.004

草地学报 ›› 2024, Vol. 32 ›› Issue (4): 1012-1020.DOI: 10.11733/j.issn.1007-0435.2024.04.004

• 研究论文 • 上一篇

接种丛枝菌根真菌对不同生育期牧草生长的影响

李春玥, 秘一先, 杨高文, 刘楠, 张英俊

中国农业大学草业科学与技术学院, 北京 100193

收稿日期:2023-12-27 修回日期:2024-02-23 发布日期:2024-05-07
通讯作者: 张英俊,E-mail:zhangyj@cau.edu.cn
作者简介:李春玥(1998-)，女，汉族，山东济南人，硕士研究生，主要从事退化草地修复研究，E-mail:1149019081@qq.com
基金资助:
国家自然科学基金重大项目(32192462)；内蒙古自治区科技计划项目(2022YFDZ0101-01)；内蒙古自治区科技计划项目(2022YFDZ0101)；财政部和农业农村部：国家现代农业产业技术体系资助

Growth Response of Reseeding Species at Different Period to Mycorrhizal Inoculation

LI Chun-yue, BI Yi-xian, YANG Gao-wen, LIU Nan, ZHANG Ying-jun

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Received:2023-12-27 Revised:2024-02-23 Published:2024-05-07

摘要/Abstract

摘要： 为探究接种丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)对不同生育期牧草生长的影响，本研究选取无芒雀麦(Bromus inermis)、冰草(Agropyron cristatum)、羊草(Leymus chinensis)和草地早熟禾(Poa pratensis)4种牧草进行盆栽试验，分别在苗期、拔节期和刈割后拔节期进行取样，测定植物生物量、菌根侵染率和菌丝密度等指标。结果表明：与不接种AMF相比，接种AMF显著提高了无芒雀麦和羊草的地上与地下生物量，但对刈割后拔节期冰草和草地早熟禾的地上和地下生物量无显著影响；4种牧草在不同生育期下菌根响应均为正效应，但是刈割后拔节期冰草、羊草和早熟禾菌根生长响应显著降低；4种牧草根系的菌根侵染率随生育期均呈增加趋势；刈割后拔节期土壤菌丝密度达到最高。因此，AMF与植物的共生关系具有物种特异性，共生作用随着生育期发生改变，随着生育期根内和根外AMF丰度增加，但菌根生长响应下降，说明AMF对植物幼苗有更强的促生作用，而这种促生作用与AMF丰度无关。

关键词: 丛枝菌根真菌, 退化草地, 草地修复, 无芒雀麦, 冰草, 羊草, 草地早熟禾

Abstract: To uncover the effects of arbuscular mycorrhizal fungi (AMF) on reseeding species at different growth period,a microcosm experiment was carried out using Bromus inermis, Agropyron cristatum,Leymus chinensis,and Poa pratensis as test material. This experiment was set up as a factorial design containing two AMF inoculation treatments (with AMF,without AMF) and three growth period (seedling,jointing,jointing after mowing). We measured above-ground and below-ground biomass,root colonization,soil hyphal density,mycorrhizal growth response,and plant-available phosphorus. Our results showed that AMF inoculation increased the above biomass and below biomass of B. inermis and L. chinensis,compared with none inoculation. However,AMF inoculation did not affect the above biomass and below biomass of A. cristatum and P. pratensis at jointing after mowing period,compared with none inoculation. Mycorrhizal growth response (MGR) of four species was positive at each period,and the lowest MGR of A. cristatum,L. chinensis,and P. pratensis was at jointing period after mowing. From seedling to jointing period,AMF root colonization of these four species significantly increased. Soil hyphal density in B. inermis, A. cristatum,and L. chinensis reached the maximum level at jointing period after mowing. Therefore,the symbiotic relationships between AMF and plants depend on growth periods of plants and were species specific. Overall,from seedlings to adults,the abundance of AMF in roots and outside roots increased,while the response of MGR showed a decreasing trend. This indicated that AMF had a strong growth-promoting effect on plant seedlings,and this growth-promoting effect was not related to AMF abundance.

Key words: AMF, Degraded grassland, Grassland restoration, Bromus inermis, Agropyron cristatum, Leymus chinensis, Poa pratensis

中图分类号:

S812.6

李春玥, 秘一先, 杨高文, 刘楠, 张英俊. 接种丛枝菌根真菌对不同生育期牧草生长的影响[J]. 草地学报, 2024, 32(4): 1012-1020.

LI Chun-yue, BI Yi-xian, YANG Gao-wen, LIU Nan, ZHANG Ying-jun. Growth Response of Reseeding Species at Different Period to Mycorrhizal Inoculation[J]. Acta Agrestia Sinica, 2024, 32(4): 1012-1020.

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参考文献

[1] O'CONNOR P J,SMITH S E,SMITH F A. Arbuscular mycorrhizas influence plant diversity and community structure in a semiarid herbland[J]. The New Phytologist,2002,154(1):209-218
[2] RILLIG M C. Arbuscular mycorrhizae and terrestrial ecosystem processes[J]. Ecology Letters,2004,7(8):740-754
[3] JOHNSON N C,HOEKSEMA J D,BEVER J D,et al. From lilliput to brobdingnag:extending models of mycorrhizal function across scales[J]. Bioscience,2006,56(11):889-900
[4] DER HEIJDEN,V. M G A,BAKKER R,et al. Symbiotic bacteria as a determinant of plant community structure and plant productivity in dune grassland[J]. Fems Microbiol Ecology,2006(56):178-187
[5] KIERS E T,DUHAMEL M,BEESETTY Y,et al. Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis[J]. Science,2011,333(6044):880-882
[6] WU Q,SRIVASTAVA A K,LI Y. Effects of mycorrhizal symbiosis on growth behavior and carbohydrate metabolism of trifoliate orange under different substrate P levels[J]. Journal of Plant Growth Regulation,2015,34(3):499-508
[7] TRESEDER K K. The extent of mycorrhizal colonization of roots and its influence on plant growth and phosphorus content[J]. Plant and Soil,2013,371(1-2):1-13
[8] HODGE A,STORER K. Arbuscular mycorrhiza and nitrogen:implications for individual plants through to ecosystems[J]. Plant and Soil,2015,386(1-2):1-19
[9] 熊天,梁洁良,李金天,等. 丛枝菌根真菌数个种的基因组和转录组研究概况[J].微生物学报,2021,61(11):3413-3430
[10] LUO N,LI X,CHEN A Y,et al. Does arbuscular mycorrhizal fungus affect cadmium uptake and chemical forms in rice at different growth stages?[J]. Science of the Total Environment,2017,599-600:1564-1572
[11] DER HEIJDEN M G A,HORTON T R. Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems[J]. Journal of Ecology,2009,97(6):1139-1150
[12] BERGER F,GUTJAHR C. Factors affecting plant responsiveness to arbuscular mycorrhiza[J]. Current Opinion in Plant Biology,2021(59):101994
[13] FELLBAUM C R,MENSAH J A,CLOOS A J,et al. Fungal nutrient allocation in common mycorrhizal networks is regulated by the carbon source strength of individual host plants[J]. New Phytol,2014,203(2):646-656
[14] JOHNSON N C,WILSON G,WILSON J A,et al. Mycorrhizal phenotypes and the Law of the Minimum[J]. New Phytol,2015,205(4):1473-1484
[15] HOEKSEMA J D,CHAUDHARY V B,GEHRING C A,et al. A meta-analysis of context-dependency in plant response to inoculation with mycorrhizal fungi[J]. Ecology Letters,2010,13(3):394-407
[16] JIN L,WANG Q,WANG Q,et al. Mycorrhizal-induced growth depression in plants[J]. Symbiosis,2017,72(2):81-88
[17] 资丽媛,林浴霞,傅若楠,等. 植物激素转运研究进展[J].植物生理学报,2022,58(12):2238-2252
[18] 黄文镜,杨树华,葛红,等. AMF对观赏植物生长发育影响的研究进展[J].中国农学通报,2023,39(7):55-63
[19] 岳丽楠,师尚礼,祁娟,等. 免耕补播对北方退化草地生产力及营养品质的影响[J].草地学报,2021,29(11):2583-2590
[20] 孙伟,刘玉玲,王德平,等. 补播羊草和黄花苜蓿对退化草甸植物群落特征的影响[J].草地学报,2021,29(8):1809-1817
[21] 程映杰,李敏,朱慧森,等. 香豆素对草地早熟禾等6种补播材料种子萌发特性的影响[J].草地学报,2022,30(10):2685-2691
[22] 魏学红,杨富裕,孙磊. 补播和施肥对藏北高寒退化草地的改良效果[J].安徽农业科学,2010,38(32):18155-18156
[23] JOHNSON N C,WILSON G W T,BOWKER M A,et al. Resource limitation is a driver of local adaptation in mycorrhizal symbioses[J]. Proceedings of the National Academy of Sciences,2010,107(5):2093-2098
[24] PHILLIPS J M,HAYMAN D S. Improved procedures for clearing and staining parasitic andvesicular-arbuscular mycorrhizal fungi for rapid assessment of infection.[J]. Proceedings of the National Academy of Sciences,2010,107(5):2093-2098
[25] 王思雨,魏涵,陈科宇,等. 丛枝菌根真菌(AMF)孢子、菌丝密度及侵染率定量测定方法[J].Bio-Protocol,2022(1):2104253
[26] 鲁佳男. 丛枝菌根真菌对3种草地植物生长的影响[D]. 保定:河北农业大学,2023:31-33
[27] 杨高文. 菌根真菌与氮磷对针茅草原群落生产力变化的作用机制[D]. 北京:中国农业大学,2014:74-94
[28] WILSON G W T,HARTNETT D C. Interspecific variation in plant responses to mycorrhizal colonization in tallgrass prairie[J]. American Journal of Botany,1998(85):1732-1738
[29] 王浩,吴爱姣,刘保兴,等. 菌根真菌多样性与植物多样性的相互作用研究进展[J].微生物学通报,2020,47(11):3918-3932
[30] 阚海明,徐恒康,鲁佳男,等. 不同丛枝菌根真菌接种对3种草地植物生长特性的影响[J].草地学报,2023,31(7):1922-1930
[31] 李侠,叶诚诚,张俊伶,等. 丛枝菌根真菌侵染指标与植物促生效应相关性分析[J].中国农业大学学报,2021,26(10):41-53
[32] 舒波,李伟才,刘丽琴,等. 丛枝菌根(AM)真菌与共生植物物质交换研究进展[J].植物营养与肥料学报,2016,22(4):1111-1117
[33] 李月灵,金则新,罗光宇,等. 干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响[J].应用生态学报,2022,33(4):963-971
[34] 辜夕容,倪亚兰,江亚男,等. 接种双色蜡蘑对马尾松根际土壤无机磷和活性铝含量的影响[J].土壤学报,2018,55(5):1179-1189

接种丛枝菌根真菌对不同生育期牧草生长的影响

Growth Response of Reseeding Species at Different Period to Mycorrhizal Inoculation

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