柱花草SgALMT1基因克隆与表达分析

doi:10.11733/j.issn.1007-0435.2024.04.010

草地学报 ›› 2024, Vol. 32 ›› Issue (4): 1078-1086.DOI: 10.11733/j.issn.1007-0435.2024.04.010

• 研究论文 • 上一篇

柱花草SgALMT1基因克隆与表达分析

缪野^1,2,3, 刘怀锦¹, 刘莉婷¹, 罗丽娟¹, 陈志坚^2,3

1. 海南大学热带农林学院, 海南海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室, 海南海口 571101;
3. 热带作物生物育种全国重点实验室, 海南三亚 572024

收稿日期:2023-12-24 修回日期:2024-02-23 发布日期:2024-05-07
通讯作者: 陈志坚,E-mail:jchen@scau.edu.cn
作者简介:缪野(1999-)，女，汉族，四川内江人，硕士研究生，主要从事热带牧草抗逆研究，E-mail：3326848781@qq.com
基金资助:
国家自然科学基金项目(31861143013，31672483)；财政部和农业农村部：国家现代农业产业技术体系(CARS-34)资助

Cloning and Expression Analysis of SgALMT1 in Stylosanthes guianensis

MIAO Ye^1,2,3, LIU Huai-jin¹, LIU Li-ting¹, LUO Li-juan¹, CHEN Zhi-jian^2,3

1. College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan Province 570228, China;
2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affair, Haikou, Hainan Province 571101, China;
3. National Key Laboratory for Tropical Crop Breeding, Sanya, Hainan Province 572024, China

Received:2023-12-24 Revised:2024-02-23 Published:2024-05-07

摘要/Abstract

摘要： 为探索铝激活苹果酸转运蛋白(Aluminum-activated malate transporter，ALMT)在植物适应酸性土壤铝毒害中的重要作用，本研究以柱花草(Stylosanthes guianensis)为研究材料，克隆了柱花草SgALMT1基因，并进行了生物信息学、亚细胞定位及基因表达分析。结果表明：SgALMT1基因全长为1 350 bp，编码450个氨基酸残基，蛋白分子量为50.6 kDa，包含6个跨膜结构域；亚细胞定位分析表明，SgALMT1蛋白定位在拟南芥原生质体细胞膜上，为膜蛋白。定量PCR结果表明，SgALMT1基因在柱花草叶中的表达量高于根中的表达量；铝处理增强了SgALMT1基因在柱花草根中的表达，表明SgALMT1基因可能参与了柱花草应对铝胁迫；另外，缺氮处理增强了SgALMT1基因在柱花草叶中的表达，而缺磷处理增强了SgALMT1在根中的表达。本研究结果为解析柱花草响应铝毒害和养分缺乏的机理提供了候选基因资源。

关键词: 柱花草, 铝毒害, 基因表达, 苹果酸, 亚细胞定位

Abstract: To investigate the important role of aluminum (Al)-activated malate transporter (ALMT) in plant adaptation to Al toxicity in acidic soils,SgALMT1 gene was cloned from stylo (Stylosanthes guianensis),its bioinformation,subcellular localization and expression pattern were subsequently analyzed in this study. The results showed that the SgALMT1 gene was 1 350 bp in length,encoding 450 amino acids. The SgALMT1 protein possessed a molecular weight of 50.6 kDa and was predicted to include six transmembrane domains. Subcellular localization analysis in Arabidopsis protoplasts showed that SgALMT1 was localized on the plasma membrane,suggesting it was a membrane protein. RT-qPCR analysis demonstrated that the expression of SgALMT1 in leaves was higher than that in roots. Al treatment enhanced the expression of SgALMT1 in roots of S. guianensis,suggesting that SgALMT1 may be involved in the response of S. guianensis to Al toxicity. In addition,the transcripts of SgALMT1 in leaves was increased by nitrogen deficient treatment,while its expression was up-regulated in roots by phosphorus deficiency. Taken together,the study provides a candidate gene for dissecting the adaptative mechanism of S. guianensis to Al toxicity and nutrient deficiency.

Key words: Stylosanthes guianensis, Aluminium toxicity, Gene expression, Malate, Subcellular localization

中图分类号:

S541

缪野, 刘怀锦, 刘莉婷, 罗丽娟, 陈志坚. 柱花草SgALMT1基因克隆与表达分析[J]. 草地学报, 2024, 32(4): 1078-1086.

MIAO Ye, LIU Huai-jin, LIU Li-ting, LUO Li-juan, CHEN Zhi-jian. Cloning and Expression Analysis of SgALMT1 in Stylosanthes guianensis[J]. Acta Agrestia Sinica, 2024, 32(4): 1078-1086.

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柱花草SgALMT1基因克隆与表达分析

Cloning and Expression Analysis of SgALMT1 in Stylosanthes guianensis

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