草地学报 ›› 2024, Vol. 32 ›› Issue (8): 2419-2427.DOI: 10.11733/j.issn.1007-0435.2024.08.009

• 研究论文 • 上一篇    

紫花苜蓿种子产量与大小相关性状全基因组关联分析

徐明1, 蒋学乾2, 何飞2, 张帆2, 杨天辉3, 龙瑞才2, 杨青川2, 丛丽丽1   

  1. 1. 青岛农业大学草业学院, 山东 青岛 266109;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
    3. 宁夏农林科学院动物科学研究所, 宁夏 银川 750002
  • 收稿日期:2024-01-29 修回日期:2024-03-22 发布日期:2024-09-07
  • 通讯作者: 杨青川,E-mail:yangqingchuan@caas.cn;丛丽丽,E-mail:congli1985610@126.com
  • 作者简介:徐明(1998-),男,汉族,山东烟台人,硕士研究生,主要从事紫花苜蓿育种方面的研究,E-mail:xuming199836@163.com
  • 基金资助:
    中国农业科学院科技创新工程项目(ASTIP-IAS14);国家牧草产业技术体系项目(CARS-34)资助

Genome-Wide Association Study (GWAS) of Seed Yield and Size-Related Traits of Alfalfa (Medicago sativa L.)

XU Ming1, JIANG Xue-qian2, HE Fei2, ZHANG Fan2, YANG Tian-hui3, LONG Rui-cai2, YANG Qing-chuan2, CONG Li-li1   

  1. 1. College of Grassland Science, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Institute of Animal Science, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, Ningxia 750002, China
  • Received:2024-01-29 Revised:2024-03-22 Published:2024-09-07

摘要: 紫花苜蓿(Medicago sativa L.)作为一种营养价值高、适口性好的豆科牧草,在我国种植面积不断扩大,其优质种子的需求量也逐年增多。因此,提高单位面积的种子产量对我国紫花苜蓿产业的发展具有重要意义。为挖掘调控种子产量与外形的相关基因,本研究中,利用从世界各地收集的220份紫花苜蓿种子材料,对其种子重量(Seed weight,SW)、种子面积(Seed area,SA)、种子周长(Seed perimeter,SP)进行全基因组关联分析(Genome-wide association study,GWAS)。表型分析表明3个性状存在不同程度的变异,变异系数分别为10.96%(SW),8.31%(SA),4.43%(SP)。通过GWAS,共在7条染色体上发现20个与3个性状相关的单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,单倍型分析证实了优异等位基因的数量与SW,SA和SP之间存在正相关性。对这20个显著SNP上下游各20 kb范围内的基因进行搜索注释,最终筛选到4个与种子产量和大小相关的候选基因。本研究的结果可为优化紫花苜蓿种子大小,提高种子产量的分子育种提供新的标记与候选基因。

关键词: 紫花苜蓿, 全基因组关联分析, 种子大小, 种子产量

Abstract: Alfalfa (Medicago sativa L.),as a leguminous forage with high nutritional value and good palatability,has been grown in more areas of China,and the demand of high-quality seeds is increasing year by year. Therefore,increasing the seed yield per unit area is of great significance to the development of alfalfa industry in China. In order to explore the genes related to the regulation of seed yield and shape,in this study,220 alfalfa seed materials collected from all over the world were used to evaluate their seed weight (SW),seed area (SA) and seed perimeter (SP) using Genome-wide association study (GWAS). Phenotypic analysis showed that the three traits had different degrees of variation,and the coefficients of variation were 10.96% (SW),8.31% (SA),and 4.43% (SP),respectively. Through GWAS,a total of 20 single nucleotide polymorphism (SNP) sites associated with three traits were found on 7 chromosomes. Haplotype analysis confirmed that there was a positive correlation between the number of excellent alleles and SW,SA and SP. Genes near these 20 significant SNPs were searched and labeled in the upstream and downstream 20 kb range,and finally 4 candidate genes related to seed yield and size were selected. The results of this study could provide new markers and candidate genes for molecular breeding to optimize alfalfa seed size and increase seed yield.

Key words: Alfalfa, GWAS, Seed size, Seed yield

中图分类号: