美洲狼尾草种子不同发育时期的转录组分析

doi:10.11733/j.issn.1007-0435.2026.05.003

草地学报 ›› 2026, Vol. 34 ›› Issue (5): 1584-1596.DOI: 10.11733/j.issn.1007-0435.2026.05.003

• 研究论文 • 上一篇

美洲狼尾草种子不同发育时期的转录组分析

刘亚男¹, 田浩琦¹, 梁小玉¹, 胡远彬¹, 张靓¹, 冯光燕², 季杨¹

1. 四川省畜牧科学研究院, 四川成都 610066;
2. 四川农业大学草业科技学院, 四川成都 610000

收稿日期:2025-07-31 修回日期:2025-09-12 发布日期:2026-05-08
通讯作者: 季杨，E-mail:23271042@qq.com
作者简介:刘亚男（1996-），女，汉族，四川营山人，硕士，畜牧师，主要从事牧草遗传育种研究，E-mail:liuyanan0310@163.com；
基金资助:
四川省畜牧科学研究院基本科研业务费（SASA202404）；四川肉羊创新团队项目（SCCXTD-2024-14-3）；宜宾市农业科技创新项目（2024NYHZ013）资助

Transcriptome Analysis of Pennisetum glaucum Seeds at Different Developmental Stages

LIU Ya-nan¹, TIAN Hao-qi¹, LIANG Xiao-yu¹, HU Yuan-bin¹, ZHANG Jing¹, FENG Guang-yan², JI Yang¹

1. Sichuan Animal Science Academy, Chengdu, Sichuan Province 610066, China;
2. College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 610000, China

Received:2025-07-31 Revised:2025-09-12 Published:2026-05-08

摘要/Abstract

摘要： 为更好地了解美洲狼尾草（Pennisetum glaucum L.）种子发育过程中的基因调控网络，本研究以开花期（F）和灌浆期（S₁，S₂，S₃）的美洲狼尾草籽粒为材料进行转录分析，结果显示，S₁ vs F时期差异表达基因数量最多，为7166个。通路富集分析表明，植物激素信号转导、淀粉生物合成及苯丙烷生物合成在种子发育中具有重要作用，分别有160，19，51个差异基因富集到以上途径，它们在种子发育过程中阶段性高表达，其中AUX1和GID1在种子发育前期高表达，而乙烯信号通路基因（ETR，EIN2，EIN3）的表达随种子成熟上调。差异表达基因中鉴定到包含AP2/ERF、bHLH家族成员在内的859个转录因子，其主要参与植物激素信号转导、昼夜节律等与种子发育相关途径。本研究基于不同时期的转录组数据构建了美洲狼尾草种子发育基因表达谱，为进一步探究其分子机制提供参考。

关键词: 美洲狼尾草, 种子发育, 转录组测序, 差异表达基因

Abstract: To better understand the gene regulatory network during seed development in Pennisetum glaucum， this study performed transcriptome analysis using seeds at the flowering stage （F） and grain-filling stages （S₁， S₂， S₃）. The results revealed the highest number of differentially expressed genes （DEGs） in the S₁ vs F comparison， totaling 7166. Pathway enrichment analysis demonstrated that plant hormone signal transduction， starch biosynthesis， and phenylpropanoid biosynthesis played important roles in seed development， with 160， 19， and 51 DEGs enriched in these pathways， respectively. These genes exhibited stage-specific high expression during seed development； among which， AUX1 and GID1 were highly expressed in early seed development， while ethylene signaling genes （ETR， EIN2， EIN3） increased in expression as seeds matured. Among the DEGs， 859 transcription factors （TFs） were identified， including members of AP2/ERF and bHLH families， primarily involved in seed development-related pathways such as plant hormone signaling and circadian rhythm. This study constructed a gene expression profile for P. glaucum seeds development based on transcriptome data from different stages， providing a reference for further research of its molecular mechanism.

Key words: Pennisetum glaucum, Seed development, Transcriptome sequencing, DEGs

中图分类号:

S543.9

刘亚男, 田浩琦, 梁小玉, 胡远彬, 张靓, 冯光燕, 季杨. 美洲狼尾草种子不同发育时期的转录组分析[J]. 草地学报, 2026, 34(5): 1584-1596.

LIU Ya-nan, TIAN Hao-qi, LIANG Xiao-yu, HU Yuan-bin, ZHANG Jing, FENG Guang-yan, JI Yang. Transcriptome Analysis of Pennisetum glaucum Seeds at Different Developmental Stages[J]. Acta Agrestia Sinica, 2026, 34(5): 1584-1596.

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美洲狼尾草种子不同发育时期的转录组分析

Transcriptome Analysis of Pennisetum glaucum Seeds at Different Developmental Stages

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