Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro

doi:10.11733/j.issn.1007-0435.2000.02.005

›› 2000, Vol. 8 ›› Issue (2): 107-114.DOI: 10.11733/j.issn.1007-0435.2000.02.005

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Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro

Jiang Changshun¹, Zou Dongmei¹, Segenet Kelemu²

1. Tropical Field Crops and Animal Husbandry Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou City, Hainan Province 571737;
2. Topical Forages Pathology Laboratory, International Center for Tropical Agriculture,A. A, 6713,Cali,Colombia

Received:1999-12-09 Revised:2000-04-06 Online:2000-05-15 Published:2000-05-15

几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果

蒋昌顺¹, 邹冬梅¹, Segenet Kelemu²

1. 中国热带农业科学院农牧研究所, 海南省, 儋州市, 571737;
2. Topical Forages Pathology Laboratory, International Centerfor Tropical Agriculture, A.A.6713, Cali, Colombia

基金资助:
国家外国专家局和国际热带农业中心资助

Abstract

Abstract: Chinase gene expressing vectors for plant transformation were constructed,35kD protein was expressed by chitinase gene in Escherichiacoli DH5α. In vitro crude preparation of chinase has efficient inhibition on the growth of Colletotrichum gloeosporioides, which caused Stylosnathes anthracnose disease.

Key words: Chinase gene, Expressing in Escherichia coli, Inhibition on Colletotrichum gloeosporioides growth

摘要： 构建了几丁质酶基因植物表达载体,该基因在大肠杆菌中能表达35kD的蛋白质。几丁质酶粗提物在体外对柱花草胶孢炭疽病原菌有极显著的抑菌效果。

关键词: 几丁质酶, 基因表达, 炭疽病原菌, 抑菌作用

CLC Number:

Jiang Changshun, Zou Dongmei, Segenet Kelemu. Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro[J]. , 2000, 8(2): 107-114.

蒋昌顺, 邹冬梅, Segenet Kelemu. 几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果[J]. , 2000, 8(2): 107-114.

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References

[1] 蒋昌顺, 黄惠德, 邹冬梅.等, 1998.热研二号柱花草组织培养研究[J]. 草地学报, 6(1):33～37.
[2] Cameron D F, Chakraborty S, Manners J M, Edye L. 1996.Protecting stylo from anthracnose[J]. Tropical Grassland, 1:130～131.
[3] Chakrabortys S, Cameron D F, Boland R, Kelemu S, Fernandes C D, Charchar M J D A.1996.Risk of serious anthracnose damage to stylo cultivars[J]. Trop Grassl, 30(1):129～130.
[4] Chakraborty S, Perrott R, Charchar M J D A, Fernandes C D, Kelemu S.1997.Biodiversity, epidemioloy and virulence of C.gloeosporioides, Ⅱ:Genetic and pathogenic diversity in isolates of C.gloeosporioides from eight species of Stylosanthes[J]. Trop Grassl, 31:393～401.
[5] Collinge D B, Kragh K M, Mikekesen J D, Niesen K K, Rasmussen U, Vad K.1993.Plant chitinases [J]. Plant J, 1:31～40.
[6] Edye L A, Cameron D F.1984.Prospects for Stylosanthes improvement and utilization.In:Stace HM, Edye LA, eds.The biology and agronomy of Stylosanthes[C]. Academic Press, North Ryde, NSW, Australia, 571～587.
[7] Huang J K, Wen L, Swegle M, Tran H C, Tin T H, Naylor H M, Muthukrishnan S, Reeck G R.1991.Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase[J]. Plant Mol Biol, 16:479～480.
[8] Inoue W.1990.High efficiency transformation procedure for E.coli.[J]. Gene (Amst), 96:23～28.
[9] Jiang G.1995.Utilization and studies of Stylosanthes in China[J]. Trop Crops Res, 3:64～70(In Chinese.).
[10] Kelemu S, Thomas R J, Moreno C X, Ocampo G I.1995.Strains of Bradyrhizobium from tropical forage legumes inhibit Rhizoctonia solani AG-1 in vitro[J]. Australas Plant Pathol, 24:168～172.
[11] Kelemu S, Badel J L, Moreno C X, Miles J W.1996.Virulence spectrum of South American isolates of Collectortrichum gloeosporioides on selected S.guianensis genotype[J]. Plant Dis, 80(12):1355～1358.
[12] Lenne J M, Sonoda R M.1982.Effect of anthracnose on yied of the tropical forage legume Stylosanthes hamata[J]. Phytopathology, 72:207～209.
[13] Lin W, Anuratha C S, Datta K, Potrykus I, Muthukrishnan S, Datta S K.1995.Genetic engineering of rice for resistance to sheath blight[J]. Biotechnology, 13:686～691.
[14] MAG (Ministério da Agricultura).1937.Informacaos sobro algumas plantas forrageiras.Riode Janeiro.
[15] Marko H.1982.Medium scale alkaline plasmid preparation procedure[J]. Annu Biochem, 121:382～383.
[16] Roberts C.1997.Acomprehensive set of modular vectors for advanced manipulations and efficient transformation of plants by both Agrobacterium and direct DNA uptake methods pCAMBIA vector release manual, version 3.05[M]. CAMBIA.Canberra.Australia, 1～6.
[17] Sambrook J, Fritsch E F, Maniatis T.1989.Molecular cloning:a laboratory manual[M]. 2nd ed.Cold Springs Harbor Laboratory Press, New York.
[18] Shapira R, Ordentlich A, Chet I, Oppenheim A B.1989.Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli[J]. Phytopathology, 11:1246～1249

Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro

几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果

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