Acta Agrestia Sinica

›› 2001, Vol. 9 ›› Issue (3): 171-175,190.DOI: 10.11733/j.issn.1007-0435.2001.03.003

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Genetic Diversity of Astragalus adsurgens Pall.Germplasm by RAPD Analysis

LI Rui-fen1, LI Cong2, SU Jia-kai2   

  1. 1. Beijing Agro-biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100089, China;
    2. Institute of Animal Science, CAAS, Beijing, 100094, China
  • Received:2000-11-16 Revised:2001-01-11 Online:2001-08-15 Published:2001-08-15

沙打旺种质资源遗传多样性RAPD分析

李瑞芬1, 李聪2, 苏加楷2   

  1. 1. 北京市农林科学院生物技术研究中心, 北京, 100089;
    2. 中国农业科学院畜牧研究所, 北京, 100094
  • 作者简介:李瑞芬(1971- ),女,助研,博士,主要从事植物远缘杂交、分子细胞遗传学和分子标记和育种工作
  • 基金资助:
    国家“九五”科技攻关专题(96-016-01-07)

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Abstract: Genetic diversity of Astragalus adsurgens Pall.Germplasm(bred varieties,local materials and wild materials)was firstly analysed by RAPD.The results indicated that DNA polymorphism of wild materials was larger than of bred varie ties and local materials.Among 13 varieties(materials),gene diversity of Shanxi Qinshui was the largest,proportion of polymorphic loci of ShanxiWutaishan was the highest.The total gene diversity and average of intramaterial gene diversity of wild materials were all larger than of bred varieties and local materials.The range of genetic variation within wild materials was the largest,the range of genetic variation within bred varieties was the smallest.Genetic differentiation among wild materials was obvious and gene differentiation coefficient of it was larger than of bred varieties and local materials.

Key words: Astragalus adsurgens Pall, Cultivated type, Wild type, Genetic diversity, RAPD

摘要： 利用RAPD技术对我国沙打旺育成品种、地方材料和野生材料的遗传多样性进行研究。结果表明,沙打旺野生材料的多态性明显高于育成品种和地方材料,在13份品种(材料)中,山西沁水野生沙打旺基因多样性最大,山西五台山野生沙打旺多态位点比例最高。沙打旺野生材料总基因多样性和平均基因多样性均大于育成品种和地方材料,其遗传变异幅度最大, 而育成品种最小。沙打旺野生材料间遗传分化明显,其基因分化系数比育成品种间和地方材料间大。

关键词: 沙打旺, 栽培型, 野生型, 遗传多样性, RAPD

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