Acta Agrestia Sinica

›› 2003, Vol. 11 ›› Issue (1): 63-66.DOI: 10.11733/j.issn.1007-0435.2003.01.013

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A Preliminary Study of Callus for Inducing Cold Tolerance in Alfalfa Breeding

LI Bo1, LI Hong2, WANG Xiu-li1, YU Fei1   

  1. 1. Life Science and Engineering College, Qiqihar University, Qiqihar, Heilongjiang Province, 161006, China;
    2. Heilongjiang Institute of Animal Science, Qiqihar, Heilongjiang Province, 161006, China
  • Received:2002-08-22 Revised:2002-12-03 Online:2003-02-15 Published:2003-02-15

苜蓿抗性愈伤组织诱导的初步研究

李波1, 李红2, 王秀丽1, 于非1   

  1. 1. 齐齐哈尔大学生命科学与工程学院, 黑龙江, 齐齐哈尔, 161006;
    2. 黑龙江省畜牧研究所, 黑龙江, 齐齐哈尔, 161000
  • 作者简介:李波(1962- ),女,辽宁省鞍山市人,副教授,从事牧草抗性研究,植物组织培养和细胞生物学教学与研究
  • 基金资助:
    黑龙江省教育委员会科研基金资助项目(2002)

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Abstract: MS was used as a fundamental medium for alfalfa breeding.DES and UV were successively used for callus mutation and selection.Eventually,some callus showed certain degree of cold tolerance.

Key words: Alfalfa, Callus, DES, UV

摘要： 以硫酸二乙酯(DES)和紫外线(UV)对准格尔苜蓿愈伤组织进行诱变处理,筛选其半致死浓度和剂量。对诱变处理后的材料进行脯氨酸含量测定。结果表明,脯氨酸含量明显高于对照。筛选出具有抗性的苜蓿愈伤组织,为苜蓿抗性育种提供基础材料。

关键词: 苜蓿, 愈伤组织, 硫酸二乙酯, 紫外线

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